U86 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101623

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE2 [Human herpesvirus 7], codon optimized for human cell expression, YP_073823



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CNY 18,240.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101623 represents NCBI reference of YP_073823 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCACAACAGCCGACGTGGTACGGGAAGCAATGACTCTCTCTGGTCTCTACAATGAGATTCACGACC
ACACTAAGCCTGAGAACGAAATTACGTACAGCAACTTTAACAACCAGGAAATAGTTACCAGCAAAACGTT
GCAAGACCCCAGGCGCGCGATACCGCTGAAAAGCCGGTCAACCTTTACATCCGAAAGTTCACCCAACACT
ACCGAAACTGGCCAGTTCAGATTTGCTTCCGAACTGCCTCATCAGGGACTCCAGAGTCTGTACTACAATT
ACCCTCAAGCATCTGGGGAGAACTTCATTTCACTGCCTTATCAGCCCCAGAGCTCAGTTGATATTTTTAA
CAACTTGGACTATAAAAATAGAGAGCTGTGCCACAACTCTTTCGATACTACATACCGCCAGCACCACTAC
CGCGGCCGCAATAACCGGGGCAAGTACGATAGCCGGAATTATTGCAACCCCAACTCAAAATACCAGAACC
ACTACGGCAAGCATTTTCACAGACCATACAACCGAAGGGGCCGCGGGTATGCGAAGTTCCACGATTTCAG
CAACAGAAATGAGTTCACATTTCCTGAGTATATGCAGCGCGACCAGAGCTATGACAGCTACAATTCCGAC
TTTAGACACAACCCATATAGGGACAATTACCAGCAACTCTGTCCCAACTACGGATTCCAGTCTAGGGACT
ACAGCATGGCTTTTTCTCAGATTCCCTCCAGTTCAATGATCACAGACTCACAAAACCTGCAGAAAGAGTG
TATTTCTAGCGGGCTGACACTGAGTAAAAAGTCCGAATACAGCCAAAATACTCCCATACAGACCAACCAT
AAAGAGACTAAACTTATATCTAATGTCAATAATCTCGAAAAACCAGCTAGGAAAAAAACTGACAAAGACA
AGACCCCACAAAATGAAGATGTGGTGTCACTCTCATATATCCCCAGCAGTCATAAAATGGATCTGATCAA
AAGGATCTATAATGCTGACATTGTGCCTCTCGCAAAGGACTCTCTGAGCTCAACCTCCGATGTCGACAAG
TGTAAGTACAATAAAAGTTCTCAAGAGAAAACTTTTATGAAGGCGCGGACTCGCCCCTCAACCTCTAAGG
AGAAGCAAATTACGTCCACAACAGATTCAAAACATTGTCCCAATCTGGACAAAGGAAGTAGCAACCTGAA
GAAAAAGGATAAGTCACCTGACGAGGCCATTGAGAACCTTAGAAAGCTCTCCAAGCTTGAGCCCATGCTG
AGCCCTTTGCCACTTACACCCAAGCCAGACGTCCAAGAGAGTAACCAGAGAGCCCCAACAAAGCTCATTA
ACTTTAAGCGGCTGCTCGAGAAAAACGACAAGGACATATCTGCCAGCGATATCGAGTTTATAAAACAGAG
CAGCCGGTGTACTGTGAGTACAGCACTCAACTGTAATTCTTCAACCGTGTTTTCCGACACAAAGTGCACT
GGAAAGAACTCTTATCGCGTCGGCCGACCACGCCAAGCCAAGACTACCGTAAATTCTAAGATCGATGCTA
AAGATAACGATTTTGAGGGAAAGGTAACAGCCCCCTGCAACATCCCCTCAATCGACAATCCTCCCGTGGA
GTCAGAGAAAAGTAATACGACAGATTCTATGTGCAACGATAATTTGACGCAAAGGACGCGGTCAGACAGC
TCCTCCACTGAAGAGGGACAATATAAGAGCCCTGTGAATGATTACTCTCAGAGTGAGGTGGAGCATGTAC
ACAGCATCGAAAACCAGCATATGGAACAAATCATTTCCCAGACTACCGATGAAATTGCTCAAAACTTCGT
CAGCCCAGAGCAGGAATTTTTGGCCAAGTCAGACACTGAGAATGGCCTGTTCGAGTTTTTCGACAACGAG
CTGCAATGCACCCAGAATTCCTCTGAAACAAAGAAGTTCCCAATCAGCGACAGAAGCATGAGTCCCAAGA
GTACACGGAGCTCAAGTTCTGACAGCGATACCGACACCAGTTCAAGCTCTTCTTCCTCCAGCTCAAAATG
TTCCTGCAAATCTAGTTCCTCAGCAAGCTCCAAGAACTCCGTGCTTAGTAGGAACTCTTGCTCCAGCACA
GAGCTGAAACCCTCATACACCGAATCTCCGAACTCCAATGGAAATAGGCAGTGCGATTTCGTGTCAAAAG
TGGACATGTCTGAGTTTGAATTTAGGTGTGGGAAAGAGCTTAACTTGTACAAACACGTGGAGAGCCGGCT
GCAGCTTGATCAAAACAACGAACACCTGTACAATCCTCGATTTCGCCCCCACATCCGCAAGAAGAGGCCA
CCAAGCACCGAGAGCGAAAGCGACACCAATTCCGATCTCCAGAGCCAGAGTTTCTCATCCAGCAAAAAGC
GCCGCCATGAACACAGGAATACAGAATCAGAGAGTTGTTCCGAGTCCACCAAGACAAGACACCGCCAAAA
GCATAGATCCAGAAGTAGTAGCGACACTTCCAGGAGTCGAAGCGCTTCAAAGTCACGAACCTGCAAAGTG
TGTAGTAATAACAAACAGGATAGCCAGTCCGAAACACAGTGTAAGCATCTCATCTCCATATCACGCAGCA
ACTCAGAACATAGTATAAGTGAAAGCACGTACCAGTCTTGTAAGAACAAAAACAGTGAAACACTCCGGTC
TCGGAGTAGGAGCTCCGAGTCTGATTGCAAGAAACGGTCTGAGCGGAGCTGTAGCAATCACTCCTGGTCC
AGCGGCAGTACCCAGAAGTCCAAACGGGTTGATATCGACTTCACCCATGGAGCCAAGTCCATTCCAAACG
AGTATGAGCAGAAAAAAAGCACAAACAAGTTTTTCAAAAATAAGAGAAGGAACGCTAGGAAGCGGTTCTC
TGATGATAGCGATTCTAGTATGCAATTGTTTTACAAGAAGCGGATTTCCGGCACACAGAAGAGTGATTCA
GAGCTGAGCGACAACGACCTGTCCTATAGAGAATACGTCAAACTGCAAGAACAGAAGCAGTCCGCCAAGT
TCAAGGTCCAGCGGGGGCGGGTTCCTACTAAGGACTTTGAGAAACTGTTTCGGAAAACCATAAGGGCATT
CGAATACAAACAGATCCCAAAGAAGCCGTTCCCCGAGGCCAAGCTGAAAGAGTCAGTGTATGCACTGTGC
TGCAATGGATCCTCTAAATCTGGCGCACTGATCATCTACTTCACGAGAAGCAAAACTGTGGCTGAGGATA
TCAAGGCCATGCAGAAAGAGTTGATGGTCCGGCCAAATATCACTATGTCTGAACCATTTAAGATGAACCA
CGCTCCACCTAGATATTACGATAAGGACAGTATAAAAAATTTTATTGAGCTTCAGAAGAAAGGACCTCAC
GAATTGTGGGAGAATATCTCTAACTCTGCTCATACCCTCTACACCAGACATTCCGACCTGAAAACAATAA
TAATCTACGCAGCCACTCCCATTGACTTCTTTATGGCCGCAAAAATCTGCAATAACTACGCTAAAAAACG
CCCGAAGGAGATCGTGCTGCGGCTGTCTAGCATTACCGACGGAGACAACCCAATCTCTATCTATAACCCC
GTCAGCCGCGATTTTCTCTCTAAATATACCGCACTTTGCAAGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101623 representing YP_073823
Red=Cloning sites Green=Tags

MSTTADVVREAMTLSGLYNEIHDHTKPENEITYSNFNNQEIVTSKTLQDPRRAIPLKSRSTFTSESSPNT
TETGQFRFASELPHQGLQSLYYNYPQASGENFISLPYQPQSSVDIFNNLDYKNRELCHNSFDTTYRQHHY
RGRNNRGKYDSRNYCNPNSKYQNHYGKHFHRPYNRRGRGYAKFHDFSNRNEFTFPEYMQRDQSYDSYNSD
FRHNPYRDNYQQLCPNYGFQSRDYSMAFSQIPSSSMITDSQNLQKECISSGLTLSKKSEYSQNTPIQTNH
KETKLISNVNNLEKPARKKTDKDKTPQNEDVVSLSYIPSSHKMDLIKRIYNADIVPLAKDSLSSTSDVDK
CKYNKSSQEKTFMKARTRPSTSKEKQITSTTDSKHCPNLDKGSSNLKKKDKSPDEAIENLRKLSKLEPML
SPLPLTPKPDVQESNQRAPTKLINFKRLLEKNDKDISASDIEFIKQSSRCTVSTALNCNSSTVFSDTKCT
GKNSYRVGRPRQAKTTVNSKIDAKDNDFEGKVTAPCNIPSIDNPPVESEKSNTTDSMCNDNLTQRTRSDS
SSTEEGQYKSPVNDYSQSEVEHVHSIENQHMEQIISQTTDEIAQNFVSPEQEFLAKSDTENGLFEFFDNE
LQCTQNSSETKKFPISDRSMSPKSTRSSSSDSDTDTSSSSSSSSSKCSCKSSSSASSKNSVLSRNSCSST
ELKPSYTESPNSNGNRQCDFVSKVDMSEFEFRCGKELNLYKHVESRLQLDQNNEHLYNPRFRPHIRKKRP
PSTESESDTNSDLQSQSFSSSKKRRHEHRNTESESCSESTKTRHRQKHRSRSSSDTSRSRSASKSRTCKV
CSNNKQDSQSETQCKHLISISRSNSEHSISESTYQSCKNKNSETLRSRSRSSESDCKKRSERSCSNHSWS
SGSTQKSKRVDIDFTHGAKSIPNEYEQKKSTNKFFKNKRRNARKRFSDDSDSSMQLFYKKRISGTQKSDS
ELSDNDLSYREYVKLQEQKQSAKFKVQRGRVPTKDFEKLFRKTIRAFEYKQIPKKPFPEAKLKESVYALC
CNGSSKSGALIIYFTRSKTVAEDIKAMQKELMVRPNITMSEPFKMNHAPPRYYDKDSIKNFIELQKKGPH
ELWENISNSAHTLYTRHSDLKTIIIYAATPIDFFMAAKICNNYAKKRPKEIVLRLSSITDGDNPISIYNP
VSRDFLSKYTALCKF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 3615 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073823
RefSeq ORF 3615 bp
Locus ID 3289541
MW 138.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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