U11 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101551

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp150 [Human herpesvirus 7], codon optimized for human cell expression, YP_073752



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CNY 11,310.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101551 represents NCBI reference of YP_073752 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATGTCACACCTGCCCTTCGCATGGATCTCCGACGAGGCCAAGTGCTTTCTCTCCCGGTTTTTTG
AAAATATCAGCAGCCTGCCAGTGGTCGACATTAGAGAGAATCCCTGGATATTGTCTCAGTGTATCGTAAA
GACAGGGAATAGCATAAACAACGTCAAAACATTGTACAACAATCTCATCCTGTGGATCTACTTCCATCAG
ACGCTGTGCAAAAAGAAACCTGATTACGAGGAGGTATGGCAGGAAATTTTGAAGGTCCAAAAGATCTTGA
AGGACTATCTGGAGCAGAGACAGATGATCACGGACTACAGCTCCCTGACAAGCTTCAACAAAGTCGGATT
CGAAACCGAGTTCAAGAACGTGGCAAAGGACCTGCTGAAACTGGGGAGCTTCCTCAGGTGGGGGACTGTG
ACCCATGCAGCAGACTATGTGAATCTGACTACGGAAGAGCGCGCCGAGATCGGGGAGAATTTGCAGAAAG
CCAAAAATAATATGCTCAGCTTTACCATATATCAAATTGTAGACCCTTGGAATGAGAATGGATATTATGT
CACAAACATTAATCGCTTGCTGTACCTGGGAAACCTTCTCATCACTTTGCACGGAAGTTGGATGAACATG
GAAAAACTTGCTCTGAACACCATCAATGAGAAGAAAAACGCAATTCTGAAAGCAATTGAGAATAACAAAA
ACTTCGTCAGTATCTACTCCTACCAGATTCTTTCACTGCCACTGACCTCCCACAGAGTTACGAGTTTTTT
CAAAATACTGACCGAGGACTTCGACGTCATTACCAAATCACTTGAGCTGCATGCATTGCCCGTTAAATCC
ACCTGGGATGATCGAGTGAAGTTTACTCCCGAACCCATCCAAACCTTTAAAGTCCTGACTGACCTCTCCA
AAAGTTCCCTGTCAAATCAGTTTGAATCTTCCTCCAAGAAAACTTCATACGGCAGTAGCTTCAACCCCGA
ACCTTTCATTAAGACTGAACAAAGGTCTAATAACACATTGTCTAAGGATCTCTTCGTGGGTTCAGAGGAC
GGGCTGCTCTCTAGCGTGAAGAAAGATAGCATGATACTGGACGAGCCTAGAAACTCCACCTCAATAAACA
ATAGCAAAAAGATGCATCGGATTCTGCAGACCGAGATTCTGGACCTGACGGACCAAACAATGCATCGCCC
GGAAGACAAAGTGAACCAATTTAATGAAATAGCCGTGGCCCCAGACGGGATTAACCAGGTGATTGATACT
CTGAGCAAGCTGGACCTTCACAACTCCAATAAGGTTATCGACATCGTTTCTTCCCCAAAAGTGAACGTGG
TGCAATTGCCTAAAAATAAAATAGATTACCACAGTACATTCTTCCTTCCTGAGAACGAGGTCAATCGGCA
GAACGGTGTGCAGAGCAGGGACCAGCTGAGCAAGAACTCTACCAATGACCTTCAGAAGATTCTCGAATTG
CGGGAACGGATCAAGACAATCAAACAGAACAACGAGGACATCTTCAAGTTGCCAAGCGAAAAGCGCCGGA
AGGAGATAGTTCACGAAAACCTGCAGTCCTTTGACGATGAACACAACGAGATGTCCCTCCCTCCCCAGGA
TCAGAAGAGCATCAAGCAGAAAAACGGCAATAAGGCCAATTCTTCTACAAAAACCCTCAACATGATCGGG
ACTAACGACGTGAACGCCAGCATGAAGGAGAAGGAGAGCGCCAGCTCAGCCAAGAAGAACCAATTGGTCA
AAGATGTTAAGTGGACGCCATCTTCAAGCCTGCTCGATCTGAGCAGAAGGAATGATCTCCTGCAAAAAGA
GCTTTTCGAGTCAGGCCTGGGGGAGAAGGTGAAGAAGTTGCTGACTGATTTTACAGATACAATCAGCCTG
GAAGAGAGGTCCCTCAAAGACGTGTTGGAGCCACCCAAGAAGACCGACGTTTCCAATATCGCTACTTTTA
ATGACAATAATCTGAAAAACTTGCTGAACAGTCGCAAGCGCGACCCGCTCTTCCAGAACTTCTCCTTTAC
GGAGAAGATGCAGCCCGTCCGATCCCCCTTCTTTCTCCCAAACGCTGAGATTCAGGATTTCGACAGTGGA
AGCCTCCTTACCGGTAAAGAAACGCAGAATACAATCTTCGGGGCTTCAAAGGCACAGGAAGATGGAGACA
AGGATCTGATAGACCTTGAGAACTCAGTTCAAAAGGACGATGACATCGTTAACAAGTTGGTGAGTCACCT
GACTCACAGCGAAGAGGACGTCGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101551 representing YP_073752
Red=Cloning sites Green=Tags

MKMSHLPFAWISDEAKCFLSRFFENISSLPVVDIRENPWILSQCIVKTGNSINNVKTLYNNLILWIYFHQ
TLCKKKPDYEEVWQEILKVQKILKDYLEQRQMITDYSSLTSFNKVGFETEFKNVAKDLLKLGSFLRWGTV
THAADYVNLTTEERAEIGENLQKAKNNMLSFTIYQIVDPWNENGYYVTNINRLLYLGNLLITLHGSWMNM
EKLALNTINEKKNAILKAIENNKNFVSIYSYQILSLPLTSHRVTSFFKILTEDFDVITKSLELHALPVKS
TWDDRVKFTPEPIQTFKVLTDLSKSSLSNQFESSSKKTSYGSSFNPEPFIKTEQRSNNTLSKDLFVGSED
GLLSSVKKDSMILDEPRNSTSINNSKKMHRILQTEILDLTDQTMHRPEDKVNQFNEIAVAPDGINQVIDT
LSKLDLHNSNKVIDIVSSPKVNVVQLPKNKIDYHSTFFLPENEVNRQNGVQSRDQLSKNSTNDLQKILEL
RERIKTIKQNNEDIFKLPSEKRRKEIVHENLQSFDDEHNEMSLPPQDQKSIKQKNGNKANSSTKTLNMIG
TNDVNASMKEKESASSAKKNQLVKDVKWTPSSSLLDLSRRNDLLQKELFESGLGEKVKKLLTDFTDTISL
EERSLKDVLEPPKKTDVSNIATFNDNNLKNLLNSRKRDPLFQNFSFTEKMQPVRSPFFLPNAEIQDFDSG
SLLTGKETQNTIFGASKAQEDGDKDLIDLENSVQKDDDIVNKLVSHLTHSEEDVV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 2265 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073752
RefSeq ORF 2265 bp
Locus ID 3289470
MW 86.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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