U30 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101569

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 7], codon optimized for human cell expression, YP_073770



  View other clones from "Virus" (83)

Need custom modification / cloning service?
Get a free quote

CNY 14,160.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101569 represents NCBI reference of YP_073770 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTGTACCAATCTCCAGTTTGAAAGTATCCTGAATTTGCTTAAGACAGATGTATCTGACAATACAT
TTCTGACCATCACCGCCAAGATAGAAATCTTCAGTCTGGCCGCTGAATCCATTACTGCCGACAAGATACT
TAGTTTTGCCAAACTGCTGCCCATTCACAATTATCACTTTAATTTCATCAAGAATCACATGGTCTTCTAC
ATTCTGAATTACTCCACTCTCGGGTTTGCTAAGAGATTTTCAATAGCGGAAACCCTCTGTCGGGAGCTCA
AGATCTTTCATAGGACATTTCAAAGTACCTCCAAGCTGCAAAATCTGAACAACGCAGAAGTACTTTACCA
GTTCGAGAAGCTCATAGAGAGTATCCAGGTTTTCAAAGCTGAGCTTGCGAGTCCAATCGGCAAGTTGCTC
AGACAGGAGACCATGATTTATGATAAGCCCACAGAGAAGAGCGTGAAGTACGTAATCATCCAGCAGAATT
TGATTAAGATCAATAACCTGATTCAGGATTGCGAGTCTATAAAGAATTGTCGCCTGATAGCCGAGTTGAT
TGATGAGCTGTATCAAAAGGTCTACCGGTGGTTTATGCAGATCTTCACCTACGATGACATCATATTCCCA
AACGACAATTTTCTGGACCGGCTCCTGAAGATGGATTTCTGTTATACCTACTACACCGCCTCCAATCAGC
ATCTTCTCAGCCTCTTCGAACAGACAATAGATAACCAGATCTTCATTGACCCCTCTCCCTACTTTGAGAT
AAATCCTGTGTACAGCCCAGAGCTCCAGTTTATGTCCACCTTCTCATTGAAAATTTTCTCCAAAAATATC
TCTGCCAAGAATGAAGATCTTTACATCTACCCACTGCTGAAAACCAACTTCTCCATACTGACCTTTCTCT
CCCTCGAGAACATCTTTTTCCACCACGGGTTTATTTATCACATTCTGCATCGCACAAACATAACACCCAC
TGAGGAAAAAAAGCTCGGAAAAATCAACGGATTTCTCAATACGGTCATTCAACAGGTTCTGATTAAAAAA
AATAACCTTCAGATCACTTTCCCTGAGCTGCTCGACAAGATTTACCATCTCCACCGGATAGGGCTCAATA
TCGAGACTGCTCAGATCTTTCTGCAGATGCTGACAACGTACAAGCCTGCTACTACAAACAACAAGCTGCA
GACCTTTTTCACGAATTTCAGCATCATTATCTTCTCAGCCTACATCTTTTTTCTTTGTATTGAGCTGTTC
AGTCCTACCTTCATTTTCCATAACAAAAAGAAACTTATCCTCGAGAAACAGAAGTCTATCATCCTCATAC
TCGGTGAACAGTTCAGCTTCATTTGGAAAGAAGTGAACGAGATTGTAGACCTCCTTTTCTCCAGCACCGT
GACTGAAACCTATTTCAAGTTTTACAGCAAGGGGGCAGATGACTACGAGAAAGATTTTCTCTACAAGGAT
CTGATGGAGAAATGGGGCGAACTCTTTTTCCCTCTCACATATTCCATGACAACCCCTCAGAAGTACACCG
ATAAGCACGTATCTTCCACCGTCTTGAAGAATTTGTGTGACACAGCATACCAGTCTAAGATGGAGACCGC
TTATGAGAGTCTCCTGCCGTATATTACCCATCCTGAGTTCAAGTTCATTTTTATTACTCACTACGTGAGG
CCATCTCTGTCACTGATAACTAACCTCACCTTTGAGGAGATTAAAGACAACAGGAGACTGCTGATCCTGA
TTTTCGCTTGTAAACTGCTGATGCCCAGTAACTACCTCTTGTCCCACTATCTCCTTCTGTTGCATGCCTT
TACCCTCCAAATCTTCAAGGTTGATCTGGGGCACTTTTCAATAATTCACGCCATTACGCAGAAGATATTC
GACAATATCAATTCACTCACCCAGACTATCTTCATCCCGAAAACTAACTTCCTGGTGAGTCTGTTGCTCA
CAGCCTACACAGTGCATATGCAGACATATGTGAATCCCTGGATCCAAAAAACAATTTCTGAGAACATTGC
ACTCCTCAAAGAGTATATCGACTTCACAAAAAAATGCAGTAGTACTTTGGCTACCACCTGTTACTTGAAT
CTGGAAAATTTCGCCGTTAATATGTACTTTGGGAAAAACAAGGTGGGCTCAACCAGCTTGTCCGCCTTCT
ATAGAACATGTTCAAAGCTGATAGAGGAAAGTAAGCTGTTCAAGGATAGACTGCAAGAGATCAAAGTAAG
CAAAACTCTCTTCATAGAGATGCTGCAGAATGTGGTGAAGAACATCACCAAATTTAAGGACCTGGTGTCT
AATCAGACCCTCCAAAATTTCATTATCATCGTGGAGCGAATAAGCAGCCACGCAAACACAACATATCAGG
ATGTACTGAACAGCATTGACGAGTGCCACTTCTCCAACATGCAATTGATTCAATCCTTCAAGAACATAGT
GTACGTTATTGACGTCCTGAACACCAAAAACATTTTTAACTTCTCCCTGGCTAGTCAGCTGATTGAGGCA
AAAAAACTGGTGAAAAAGCAGGATACCTACAATCAACTGAACGTGCAGGATGATTTCGTAACTGTGCTGA
AGTCACACCTGAACAACCTGTTCGAGAAACAGAAACCAACTATTAACATTGAGAGACGGTTTATGCTGGA
GGGGATTCCCGATATCAAGCAGATCCCTTTCCTGGATGTGTTTGACGAGCGCTACCGGTTGATTCCACAG
ATCGAAAAATACCTGCACTGGTACATCGCCTACTCAGAGGCCGCACAGGCAGACCTGGTGGAGCCCTTGC
TCCTGAAACTGGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101569 representing YP_073770
Red=Cloning sites Green=Tags

MDCTNLQFESILNLLKTDVSDNTFLTITAKIEIFSLAAESITADKILSFAKLLPIHNYHFNFIKNHMVFY
ILNYSTLGFAKRFSIAETLCRELKIFHRTFQSTSKLQNLNNAEVLYQFEKLIESIQVFKAELASPIGKLL
RQETMIYDKPTEKSVKYVIIQQNLIKINNLIQDCESIKNCRLIAELIDELYQKVYRWFMQIFTYDDIIFP
NDNFLDRLLKMDFCYTYYTASNQHLLSLFEQTIDNQIFIDPSPYFEINPVYSPELQFMSTFSLKIFSKNI
SAKNEDLYIYPLLKTNFSILTFLSLENIFFHHGFIYHILHRTNITPTEEKKLGKINGFLNTVIQQVLIKK
NNLQITFPELLDKIYHLHRIGLNIETAQIFLQMLTTYKPATTNNKLQTFFTNFSIIIFSAYIFFLCIELF
SPTFIFHNKKKLILEKQKSIILILGEQFSFIWKEVNEIVDLLFSSTVTETYFKFYSKGADDYEKDFLYKD
LMEKWGELFFPLTYSMTTPQKYTDKHVSSTVLKNLCDTAYQSKMETAYESLLPYITHPEFKFIFITHYVR
PSLSLITNLTFEEIKDNRRLLILIFACKLLMPSNYLLSHYLLLLHAFTLQIFKVDLGHFSIIHAITQKIF
DNINSLTQTIFIPKTNFLVSLLLTAYTVHMQTYVNPWIQKTISENIALLKEYIDFTKKCSSTLATTCYLN
LENFAVNMYFGKNKVGSTSLSAFYRTCSKLIEESKLFKDRLQEIKVSKTLFIEMLQNVVKNITKFKDLVS
NQTLQNFIIIVERISSHANTTYQDVLNSIDECHFSNMQLIQSFKNIVYVIDVLNTKNIFNFSLASQLIEA
KKLVKKQDTYNQLNVQDDFVTVLKSHLNNLFEKQKPTINIERRFMLEGIPDIKQIPFLDVFDERYRLIPQ
IEKYLHWYIAYSEAAQADLVEPLLLKLG

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 2814 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073770
RefSeq ORF 2814 bp
Locus ID 3289488
MW 110.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...