U43 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101582

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 7], codon optimized for human cell expression, YP_073783



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CNY 13,020.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101582 represents NCBI reference of YP_073783 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATCACTGTGTTCGCCACAGAATATGACCCCGCACACGTTATAGTCAATATTTTGTGTAAAAACC
CTTCAGAGCACCTGATCTTTCCTATAATCGTAAAGTACAAACCTAGCAAAAACGTTTATTTTTGTATGCA
GACACAAAAGTGCAAGTTCTCCAAGCGGATTGAGACCGTATTCGTGTGCGACGCGGAGTCTCTCAACTTC
TCATATTATGTCAAGAATGCTCTGCCTATCAAGAGCGAAGACATTATCCATTGCCTGAATACCGAAGAGA
CGGAGAATCTCTACAAAGACTTCCTTCTCTCACACGTCCCTGAGGGTAGTGAGAATATCGAGTTCAAGTC
TCTGGTGTTTTTTTGCAAAACTATCATTATCAAGCACCTGACTAATAAATACCTTCTTCCCACAAGCCCC
TTCTGGTTTTTGTCCACTTACGGCCAGACAGAGGGAATGCTCCTGCTGACCATGTACTATTACCTCTTTG
AAGAACAAAAAAGCACCATTGCTACAACTAAAAATTACGTGCAATGTTTTACGGACAAGCTGGGTGACAT
GGTGTTTACCTACTCCTCCATGAGTGAATTCATTAACATCACGCTTAAGAGCAACTACAGAAAGAAATTC
GTTTCATTTTCAGAGTATGCAAAGCAAAAGAACATACGAGACCGAAAGGAATTCCTGTACCTGGACAAGC
AGATCGACATCTTTCGCAATTCCGTGCACCTCACTAATTCCTTCCGAGTACATTACATCTATATTGCCTA
CAGTACCGCACTGGAAAAGAATAAGTTCATCAAGTATAGCCAGCTTACATCTTATGACCCTACACGATCT
GACACTTCCCAGTGCCAGGAGAACATGTATATCTTGGGGAATAGCTTGCACAGTGACCTGATATCCATCA
TGAAACAGTACTTTAATGAAGATTCCTATTTTCAGAACTACGTGGAGATTAAGCGGATGCTTAACAACAA
ATTTCAGATGCAGCAGTACGTGTACGATATCAACTCAAATCGGAACATCATGTTGGTCATCAATTCAGAT
CAGATTTCCAAGATGGTCAACAAATGTAATAAACACGGCGAGGGGTATTTCACACCTATCAAACTCGGAC
TGCAGGGGTTCTTGAAAATCCTGGCCTCCAACAAATCCATTCTTATCGATGGCAAACCAGTGACCCGGAG
GCAGTACCTCCATGACCAATTTTCTAATCCTATCCCTATGTTCCGGGTCCAGATGTCTTACAAGAATCTT
TATTGTTTCGGCTCCGCCGAGTCATGGTACAAGAACATGGGTTTTGATCAGGTGATGCAGTTTCTGCCTA
ATGAATACATTAGTGATGAATCTCTGACGAGCACCTTCTGGCTGCAGGATACAACATTCTTGTCTGACGA
GATAGAGAAACAGTTCTACGTCACGAGGCACGAAATCTTTAATGAGTACCTGCCCGTCACAAACTACATC
GGAGATCTGGATCTCCCGCTCCAGGACAGCGCCATCATCACGGAAAGTCTTTTTTTTTCCATGTGCAAGC
TGATGAGGAACGTGCTGATTAATGCTTGGCAAAAGATATTTCCCTTCATTGACAAAGATGCCTACCCCAT
ATTCTTCTTTAAAACAACATGTTCTAACCCAGAGAACCCACTGAACCACAACGTGTGTTACAACGAGGTG
GAAACCGCCTTTTGCGTATGCAAAAAGAAGATTGGCCTCCGGATTGCGATCCCCATTCCCCAAGGCACAG
CAATCATTGGAAGCGAACCGCTTAAGCAGTTGTCCAAAATCTTTAATCACCTGATGTGTCTGAACCATGA
CCTGATGCAGATACTTAATTCAGTGATTTTCCCTGGTGAGTGCTTCGACACCGGGATTTATAATACTGGA
AGGTGTTTGAGACTGGGCTTCATGTACAAAGTTGATGAAGAGAATAATCGATTTCTTTACGGGAGGCTGA
AGCCCATCTTCATCGTTCCAGAAAAGATGAAAAAAAACTTCCAAGATTTTGTTAGCATGCAGCTTGACCT
CAACAATATCCTGCATCATGGCACAAAAGACCAGACAATTACCGAGATTATTTACTCCATATTTGATAAA
GCTTGCCCAACAGAGTTTTCTTTTATCGATTCTCGCACCAAGCAACTTTATCACAGAAAGCAGAGCAGCC
TCGAAACCTTGTGCCTGAAGTACCTCCACGTGAATGGGTTCTCCGAGACATCTTGCCTGTCCCGCGATGA
TCTTCTCATGACATTTACACGGAGCATTGCCTGGCCTCAGATGCTGAAAAAAAACATCCAGCATTGTGAA
GCCAGGACCGCCACACAGTTCCAACACGTTACTTTTCTGAAAATCGATCATAAAAATATCCAGCTTAAAA
AACTCCAGAACGGCAAACTGAGTGACTTCTCCTGTCTTACACGAAACCACAAAGGCAACCGGGAAAACGT
CCTGGTGTACCTCGAATTCAAGGTGGACAACAACAGAATCCTGATCATACTGTGGTCCAAATGTTTTACA
ACAAAGTGCAAGAGCAATAGCAAGCAGGTTCACTCCAGTGTGGTGCTCGATTCCCTCAACATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101582 representing YP_073783
Red=Cloning sites Green=Tags

MTITVFATEYDPAHVIVNILCKNPSEHLIFPIIVKYKPSKNVYFCMQTQKCKFSKRIETVFVCDAESLNF
SYYVKNALPIKSEDIIHCLNTEETENLYKDFLLSHVPEGSENIEFKSLVFFCKTIIIKHLTNKYLLPTSP
FWFLSTYGQTEGMLLLTMYYYLFEEQKSTIATTKNYVQCFTDKLGDMVFTYSSMSEFINITLKSNYRKKF
VSFSEYAKQKNIRDRKEFLYLDKQIDIFRNSVHLTNSFRVHYIYIAYSTALEKNKFIKYSQLTSYDPTRS
DTSQCQENMYILGNSLHSDLISIMKQYFNEDSYFQNYVEIKRMLNNKFQMQQYVYDINSNRNIMLVINSD
QISKMVNKCNKHGEGYFTPIKLGLQGFLKILASNKSILIDGKPVTRRQYLHDQFSNPIPMFRVQMSYKNL
YCFGSAESWYKNMGFDQVMQFLPNEYISDESLTSTFWLQDTTFLSDEIEKQFYVTRHEIFNEYLPVTNYI
GDLDLPLQDSAIITESLFFSMCKLMRNVLINAWQKIFPFIDKDAYPIFFFKTTCSNPENPLNHNVCYNEV
ETAFCVCKKKIGLRIAIPIPQGTAIIGSEPLKQLSKIFNHLMCLNHDLMQILNSVIFPGECFDTGIYNTG
RCLRLGFMYKVDEENNRFLYGRLKPIFIVPEKMKKNFQDFVSMQLDLNNILHHGTKDQTITEIIYSIFDK
ACPTEFSFIDSRTKQLYHRKQSSLETLCLKYLHVNGFSETSCLSRDDLLMTFTRSIAWPQMLKKNIQHCE
ARTATQFQHVTFLKIDHKNIQLKKLQNGKLSDFSCLTRNHKGNRENVLVYLEFKVDNNRILIILWSKCFT
TKCKSNSKQVHSSVVLDSLNM

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 2583 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073783
RefSeq ORF 2583 bp
Locus ID 3289501
MW 101.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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