U26 (NC_001716) Virus Tagged ORF Clone
CAT#: VC101565
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for envelope protein UL43 [Human herpesvirus 7], codon optimized for human cell expression, YP_073766
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC101565 represents NCBI reference of YP_073766 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAACGTCATGATAAGCTTTAGCATGGGCCTTGTCCTCGGCAACGTTATTCCTGGCGAGAGCTGTT TTCATCTCATGATCAAGAAAACCCTGTTTGAACAGGTGGTCCTGATCTTTCTTTTCTCTGGTGGCCTCCT CCTTACCTACAGGCGAGTGAATCTGTTTAATTACTGCCCCTTCAACGATCTTAAGATCATCACCTTGTCT CTGCTGGTCCTCCAAATGACGGATGAGTTTTTTTTGGTGATATGTTCTTTCCTGTTTTTCTCTGAAATTC GGTTGCGGGCGATTGTTTGTAGGTGCATGCTCATATTTCCAACCCATTCTATGGCAAGTTACTTCGGCAC CTTGATCGCCATTGTGCTTAAAATCTCTGACCTTGTCCATTATCGGCTGCTGGTGACAGTGTTTCTTATA ACCCCGTATGTGACCAGCTACAAATTTCTTGTCAATTCCCACCAATTCGCTATGTGTCTCCAAAGGTACA AGCCTATCTATAAGCCGAACGGACCTTATCAAATGGCGATCAAGGACGTGATTGTGTATCTGCTTCAGTT TTTGACATTGACGCTGTTGGTCTGGTTTGGTAAACTTTATCTCCCGCGCAAGTCTTGTCAGCACCTTTTC TTTCTGGCCGTCCTCCATTCCAACCTCTTCATAACCAAGCTGTATCAGCTGGTCCTGTGCTCCATATTGT GTCTCCTGGCTGGGGTTATTACAGAACATTGTTTCTTTTCACTGCTCTTCGAGTTCTTCCTGGGACTGGG TTATTCCGCGCTCTTTGCTCAGATTTCTAAAACAGTTGGGAGGAAAGACATCTTCACAGGCGATCTGCTC AATCTCTTTTACTGTAGCGCGTGCTTCGTAATCTTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101565 representing YP_073766
Red=Cloning sites Green=Tags MWNVMISFSMGLVLGNVIPGESCFHLMIKKTLFEQVVLIFLFSGGLLLTYRRVNLFNYCPFNDLKIITLS LLVLQMTDEFFLVICSFLFFSEIRLRAIVCRCMLIFPTHSMASYFGTLIAIVLKISDLVHYRLLVTVFLI TPYVTSYKFLVNSHQFAMCLQRYKPIYKPNGPYQMAIKDVIVYLLQFLTLTLLVWFGKLYLPRKSCQHLF FLAVLHSNLFITKLYQLVLCSILCLLAGVITEHCFFSLLFEFFLGLGYSALFAQISKTVGRKDIFTGDLL NLFYCSACFVIFF TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001716 |
ORF Size | 879 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001716.2, YP_073766 |
RefSeq ORF | 879 bp |
Locus ID | 3289484 |
MW | 33.9 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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VC101562 | Myc-DDK-tagged ORF clone of viral ORF for protein U24 [Human herpesvirus 7], codon optimized for human cell expression, YP_073763 |
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VC101563 | Myc-DDK-tagged ORF clone of viral ORF for protein U24A [Human herpesvirus 7], codon optimized for human cell expression, YP_073764 |
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VC101568 | Myc-DDK-tagged ORF clone of viral ORF for capsid triplex subunit 1 [Human herpesvirus 7], codon optimized for human cell expression, YP_073769 |
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VC101569 | Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 7], codon optimized for human cell expression, YP_073770 |
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VC101622 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein UL119 [Human herpesvirus 7], codon optimized for human cell expression, YP_073822 |
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VC101623 | Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE2 [Human herpesvirus 7], codon optimized for human cell expression, YP_073823 |
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VC101624 | Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE1 [Human herpesvirus 7], codon optimized for human cell expression, YP_073824 |
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VC101625 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein UL124 [Human herpesvirus 7], codon optimized for human cell expression, YP_073825 |
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VC101626 | Myc-DDK-tagged ORF clone of viral ORF for protein U95 [Human herpesvirus 7], codon optimized for human cell expression, YP_073826 |
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VC101627 | Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein Q [Human herpesvirus 7], codon optimized for human cell expression, YP_073827 |
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VC101628 | Myc-DDK-tagged ORF clone of viral ORF for protein DR1 [Human herpesvirus 7], codon optimized for human cell expression, YP_073828 |
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VC101629 | Myc-DDK-tagged ORF clone of viral ORF for protein DR6 [Human herpesvirus 7], codon optimized for human cell expression, YP_073829 |
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