U57 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101599

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 7], codon optimized for human cell expression, YP_073799



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CNY 20,330.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101599 represents NCBI reference of YP_073799 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAATTGGAGAACTGCCGAAATTTTCCCAAAGCTGGACGTCTCCCCTAACGTGTTCGATGATATCA
GAACCCAGACCGCCGAGCAGCTCTTTGAGAATCTTCGCTTGTACTATGGTGACGATTCAGATAGATACAA
CATCAGCTTTGAGGCGCTCTTGGGAATTTATTGCAACAGGACCGAATGGATCGATTTTTTTCACACCAGT
ATTGCAGTTGCCGCCAACGTGATTAGATTCAATGACCTCGACAAGATGAGTCTCGGCAAAATCCTCTTCT
ACATTCAGCTCCCCCGGGTTGCAACCGGAAATGACGTCACTGCCCCCAAAGAAACCACTGTTCTGGTAAC
CAAATACAGTGAGAAGCACCCAATCAATATTTCCTTCGAACTGTCTGCAGCTTGCCTCGCTCATCTGGAG
AATACGTTCAAGAATACAATTCTTGACCAGATGCTGAATATCAACGCAATCCACACAGTGCTTAGGTCCC
TTAAAAATAGCGCCGACAGCCTTCAGAGAGGGTTGATCTACGCTTTTATAAAAACTATTTTGAAAAAGGC
GCCACCCCAGTTCATTCTTAAAACTATGCTGGAAAATAAAGTGAATAGTAAGCAAATTCTGTCAAAGGTG
CAAAGATCTAACATGTTTCAGAATTTCAAGAATAAGCTGATTAACAGCCTGTTTTTCCTGAACCGCACCT
CCAACGTGTCATTCATATACCGCTATCTGTGCGAGATGGTCGACAGTACAACAGAATCAATTTTGAACAA
CACCAATAGTTACGTCCTGAAGGATGGTACCCCCATCAATGGGGTCCTTCTGGGCACCCCGAATACAATT
CAAATTCTGTCCAACGCTTTGTCTCAGCACATCTCTCAGATGACCATGTCTGTGCCCGTTAGCTACGGTA
CATTCGTAATGGGGAAAGAAAACGCCGTTACAGCCATCGCCTATCAGGCTATTATGGCTGACTTCTCTAA
CTATACTAAAAACGTGGCCACAGAGACCCAGGACCAGAACAAAAAGAGTGAGATTTTTGAGAATCAGACA
CAGCATGCTGACCTGAAGACGAATATTATTCAGCTCTCCGACAAAACTGTCGTGCTGGATCATCTGAAGA
AAGTGTACAAAAATACTAACATAGAAGATCCCTTGGAGCAGAAACTGGAGCTGACTTTCTTCTTTCCCAT
GGGACTTTACATTTCCAAGGACTCTGGCTTCTCCACCATGGATAGCCGACTGAAACTGAACGACACCATG
GAGAATAACCTGCCCACTAGTATATACTTTTACAACAAGGATAAGCTGCTGCAGCGCATCGACTATTCAG
ACCTCCTGCCTTCTCTTTGTCATCCGATTATCTTTGACTGTAGCGTTTCCGAGAGAATATTCAAGAACGC
CGCGAAGCCTACAGGCGAATCATTCAATCAACTCTGTCAGGTGGAGTTCGTCCGCGAGCCACCCAGTACC
TTTCTTTCCAACTTGTACAACCTCTACGAGATGAAAAAGGAGATCCCTAAGACTACAAACATGCTGAAAA
ACGAACTGACCACCGAAGATTTCTATAAATCTGAAAATTTCACGCTGAAGACTGAACTGCACCCCTTCTT
CGACTTCACCTACATCCAGAAGAACAGGTCTACAGACGTCCTCTGCAGCCCTCGGATCCTGCTTGGTAAC
ATCCCGCTTCCCCTCGCTCCCTCTTCCTTTCACGAGGCCAGAACTAATCAGATGATAGAGCAAGCTAAGA
CAAACAACCTGAACTACGACTACACCCTCAAACTGGTCGTCGAGTCATTGACCAATACAGCCTACCCAGA
GCTCGCATACATCATTGAGCTGCTTATACATGGTAACAAGACCGCATTCCAGATCCTTAAAGATGTGGTG
AGCCAGTGTATCACATACTGGTATAACATCAAGCATATACTTCTTTTTTGTAACAACTTTGAAATGATAT
GGCTTATCACCACGTACCTCGGGGATGAGTCAATTCCCGGCATCGCGTACACTCACTACAAGAACATCAT
TAGCATACTCAAACTGGTCAAGCGGACAATAAGCATCTCCAACTTCAATGAGCAGTTGTGCGGCGAACCT
CTGGTTGGATTTGTCAATGCCCTCTTTGATAACCGCCTGTTCCCGCCATTTCTCAATTCTCTTCCCAAGA
ACGAGGCAAACGCAATAATTACGGCCGGGAACACACCCCTCACTCAGAATACCGTTAAGCTGAGAAACTA
TGAAGTCTCAGACCTCAATCGGATGAACCTCTTGGATTCTACCGAGATCTTTACTGATGTAGACCGCCCA
TCCTTCGAAACTATCGTGCTCTCCAAAATCTTCTACTTTTGCTTCCTCCCAGCTCTGACAAACAATAAAA
TGTGTGGGGCTGGATTCGACGTTAAGTCATTTATCCTGGACTTCTTCTACACGGAGCCGTTTATTCTGCC
CGATGATAACTTTTGTGAGCTTCCGATTACAAATAACGTGCTCATCGAACTGATCACAGAGGCAGTGGGA
CCTTCCCACGCCCTCACCGACCTGAGTTGTATTGGGAAGCAACTGTTCAAGAGCATCCTGTATCTGACGG
AGAACACGAAAATCCTCGAGATAGAGAGCAGTCTGGATCCTTCACAGAGACATGGGTCTTCCTCAAACTT
TAAGTCCCTGCAACACGTCCTGTATAATGGGCTGTGCCTTGTGTCTCCCATCAACGTCCTGAAACGCTAC
TTTAAACCTATACCCTTCAATCGGTTTTTTTCCGACCCGATCATTTGTGGCTTGATGAATATTGAAGTTC
AGACATACCTGAACATTTTTCCCCACTATCAGCGCAATGATGGCGGTTTTCCACTTCCCCAGGCGCTCTC
ACACGAATTCCATAATTGGCAGAGGACGCCCTTCTTCGTGTACGCATCATGCTGTAGCAACAGCCTCCTG
AGCATTATGACCCTGGCGACCATGCATTGTAAACTGTCCCCTATAGCCATCATCCTCCAAAGCCGCCAGA
AGATACACCCAGGGTTCGCCGCCACACTGGTTAGGACCGATTGCTTCGACATCAATTGCCTGCTTTACTC
ATCTAAGTCTGCCACCTCCATCATGATCGATGACCCAACAGTTAGCACTGAAGTGAAAGACATTTCAACA
ACATACAACCTCACTCAACACATTTCTTTCCTGGATATGGGACTGGGATTCTCTTCTAGCACCGCTATTG
CCAACCTGAAAAGGGTGAAGACAGACATGGGTAGCAAGGTTCAGGATCTGTTCAGCGTATTTCCTATGCA
TGCCTACACCAATCCCACGGTAAATTCCTGGGTTAGACATCATGTCGGAATCGAAAAACCCAACCCTTCA
GAAACCGATGCACTGAATATTTTGAGCTTTGGAAAAATCAACAAGCAGAGTCAATCTATTCTGCTCCACG
GGCAGCAGGCTATATGCGAGGTCGTCATAACTCCCGTAACCAGCGACATCAACTTTTACAAAACACCCAA
GAACCCGCGCGGCAGAGCTTCTTGCATGATGGGGGTGGACCCGCATAACGAGAGCGAGGCCCGGAAGTCC
CTTTACGATCATTCCCGGGTGGATTCCGACGCTTTCGTCGCAACTACTAACCCATGGGCCAGCCAGGAGG
GTTCCCTGTCTGATGTGCTCTACAACATAAATCACCGAGATCAACTCGGCTATAATCCCAAGTCCTACTC
TCCAAATGCTGTGTTCTTTACGGACACAGAGATCTTTAAGACCAACAAATTCATGTTCAAACTGATCAGT
GATTACAGTATCAAGACTAAGACCTGTCTCGACTCTGACACCGACATACAGTACTCTTGTAGCGAAGGTA
CAGACGATGTGACGCATCGGCCTTGTCAGTTTTTGCAAATCGCCTTCCCCATTCACTGTTCATCTAACCA
AGCCCTGCTGGAGTCTCGAAGCAAGAACGGAATGACACAGCTTTCCGAGACCCATTTCGCTAATTTTGCC
ATCGGTGAGTGTATACCCCTTCAGAATATAATCGAAAGCCTGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101599 representing YP_073799
Red=Cloning sites Green=Tags

MENWRTAEIFPKLDVSPNVFDDIRTQTAEQLFENLRLYYGDDSDRYNISFEALLGIYCNRTEWIDFFHTS
IAVAANVIRFNDLDKMSLGKILFYIQLPRVATGNDVTAPKETTVLVTKYSEKHPINISFELSAACLAHLE
NTFKNTILDQMLNINAIHTVLRSLKNSADSLQRGLIYAFIKTILKKAPPQFILKTMLENKVNSKQILSKV
QRSNMFQNFKNKLINSLFFLNRTSNVSFIYRYLCEMVDSTTESILNNTNSYVLKDGTPINGVLLGTPNTI
QILSNALSQHISQMTMSVPVSYGTFVMGKENAVTAIAYQAIMADFSNYTKNVATETQDQNKKSEIFENQT
QHADLKTNIIQLSDKTVVLDHLKKVYKNTNIEDPLEQKLELTFFFPMGLYISKDSGFSTMDSRLKLNDTM
ENNLPTSIYFYNKDKLLQRIDYSDLLPSLCHPIIFDCSVSERIFKNAAKPTGESFNQLCQVEFVREPPST
FLSNLYNLYEMKKEIPKTTNMLKNELTTEDFYKSENFTLKTELHPFFDFTYIQKNRSTDVLCSPRILLGN
IPLPLAPSSFHEARTNQMIEQAKTNNLNYDYTLKLVVESLTNTAYPELAYIIELLIHGNKTAFQILKDVV
SQCITYWYNIKHILLFCNNFEMIWLITTYLGDESIPGIAYTHYKNIISILKLVKRTISISNFNEQLCGEP
LVGFVNALFDNRLFPPFLNSLPKNEANAIITAGNTPLTQNTVKLRNYEVSDLNRMNLLDSTEIFTDVDRP
SFETIVLSKIFYFCFLPALTNNKMCGAGFDVKSFILDFFYTEPFILPDDNFCELPITNNVLIELITEAVG
PSHALTDLSCIGKQLFKSILYLTENTKILEIESSLDPSQRHGSSSNFKSLQHVLYNGLCLVSPINVLKRY
FKPIPFNRFFSDPIICGLMNIEVQTYLNIFPHYQRNDGGFPLPQALSHEFHNWQRTPFFVYASCCSNSLL
SIMTLATMHCKLSPIAIILQSRQKIHPGFAATLVRTDCFDINCLLYSSKSATSIMIDDPTVSTEVKDIST
TYNLTQHISFLDMGLGFSSSTAIANLKRVKTDMGSKVQDLFSVFPMHAYTNPTVNSWVRHHVGIEKPNPS
ETDALNILSFGKINKQSQSILLHGQQAICEVVITPVTSDINFYKTPKNPRGRASCMMGVDPHNESEARKS
LYDHSRVDSDAFVATTNPWASQEGSLSDVLYNINHRDQLGYNPKSYSPNAVFFTDTEIFKTNKFMFKLIS
DYSIKTKTCLDSDTDIQYSCSEGTDDVTHRPCQFLQIAFPIHCSSNQALLESRSKNGMTQLSETHFANFA
IGECIPLQNIIESLL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 4035 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073799
RefSeq ORF 4035 bp
Locus ID 3289517
MW 152.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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