U77 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101617

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase helicase subunit [Human herpesvirus 7], codon optimized for human cell expression, YP_073817



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CNY 12,450.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101617 represents NCBI reference of YP_073817 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCTGTCTTCATTGTTCAACGGAAAATACGATACCAAATTTTTGCTGAACATGAGCAGTGCGGCTA
AGGTTGAGCTGATTGTGGAAAAGGTTGCGGCCCTGGCAGATGCTTGTCTTGAAACCCCTCTTCCAACTGA
CTGGTTCCGCAATATTCTGGACCCCGAGTTGGAATTCAATTCCAACTTTGAGGAGATTCACAGCATCGGG
GACGAGGAATTCGCACAGCCCCTCCCCTTCCTGCCATTTCGCGTGCTGTTGATAACAGGCACGGCCGGCG
CCGGAAAAACTTCTTCTATCCAGACACTGGCCGCTAACAGTGACTGTCTGATTACTGCTACAACCAGCAT
CGCAGCCCAAAACCTGTCAGGGCTCCTGAACAGAACCAAATCTGCCCAGGTGAAGACGATATTTAAAACT
TTCGGCTTCAACTCCAGTCACGTGAGTATGAATGAACGGATCAGCTGCAGCGTTACAACACTGGACAGTA
TTGCAGATCAGCAGAAGCATGATCTGAGTACTTATTGGAACGTGATAGCTGATATCGCCGAAAGGGCTCT
CAACGCTGCCAACGGGAAAGCAAAGGTGATCCCAGACCTCTGTGAGTCCAGTGTGATAGTCATCGATGAG
GCCGGAGTTATTCTCCGGCATATTCTGCATACTGTGGTCTTCTTCTACTGGTTCTACAACGGCCTCCACA
AGACCCAGCTGTACAAGAACAGAGTCATTCCTTGTATTGTGTGTGTTGGCAGCCCTACGCAGTCAGGTGC
ACTGATTTCTAGCTTTAATCCCTTGACACAAAATAAGGACGTAAAAAAAGGCTTTGATATTTTGAGTGCT
CTTATATGCGATGATATCCTCAGTAACTACTGCAAAATCAGTGAGAATTGGGTGATCTTCGTAAACAACA
AACGGTGCACCGACGTGGAATTCGGGGAGTTCCTGAAACATATAGAATTTGGTCTCCCCTTGAAACCTGA
GCTTATCGAATATGTGGATCGATTTGTGCGCCCCGCCACCTATATACGCAACCCCACCAATGAGATAGGG
ATGACTCGACTCTTCCTGTCACACTACGAGGTGAAGAGTTATTTCAAAGTGCTCCATGAGCAAGTTGAGC
TGACAAACAAAGACAATCTGTTCACCTTTCCTGTATACTTCATTATTCAGAATAAGGCTTTCGAAGATTA
TAAGAACGAGATTTCTAACTTCACCCTGGAAATTGAACCATGGTTTAAAACTAATTTGCATAGGTTGAAT
ACCTATTCCCAATTTGCTGATCAGGACCTGAGCAAGACTATTCAGATCGAGGAAATCGTGCTGGACGACG
GCTCCGTGGAGGAGACACTTATCACCTGTCATCTGAAGCATATTAAGCACAGTTCAATTGGCGTTACCTC
CCGCACAAAGTCTTCTACAGTTGGCTTTTCCGGGACTTACGAAAAATTCGTGGAGCTTCTTCAAAGCGAC
CTTTTCATCGAGAAAACAGCCTGTGAATACAGCGTGCATGCTTATTCTTTTCTGACTGGACTGATGTACG
GTGGAATGTATTCTTTTTGTCTGTCCGAATTCACAACAAGTGAAGTGATGACAGAAATCCGCAAGATCAA
ACTGCCTAACATAGATTTCCTGCAGACAATGACCGCAGAAGTCTCTTTGCAGACCTTTGACGAATCAGAT
GAGTACTACGACCTTCACATTGCACCTACTGACGAAGAAATGCTGGCTAGCGATCCGTGCCCAGATCCCT
TTTTCCTGAAGTACAAGCAGCTTCCTCTCACCAACGTGCTGACTTTCGAAGAAATTTCTTACTTGTACAC
CGTGTTCAAAGAGATCTTTATCTCAAGATTTGCGATTTTGCAACGACACTCAAAAGAAATGTTTGGAAAA
AGTAATCTGATTACTTATAACCGCAACAACGTTTCTAGCAAGAGGTGTGGTGAAATCTGCTCCCACGTGA
AGAGTTTCTATGGCATGCTGACTTATGCGGTGCCCGCAAACAACTACACCCTGGAGGGATATACCTACGA
CAACGTCATTTTTCTCGGTACGGATAAGATGCTGCCCCCTATCATTTATAAGCGCGGATTGCCTAAAATC
GTCATCAAGGATGAGATGGGATTCATCTCCATTCTGGACAACAATGTGTCCAAGTTGACTGATACTGTCA
ATGGGAATTCTTTTCACATCTGTACAACGATCGATTATGCTATTGTATCCAAAGTTGCCATGACCGTTAC
TAAAAGTCAGGGTCTGTCTATACAGCGGGTGGCCTTGGATTTTGGGAACGATCCGAAGAACCTGAAACTG
AGTTCCATTTATGTGGGGATGAGCCGGGTGGTCGACCCCAACAACCTGATCATGAATCTGAATCCCTTGA
GACTGAATTACGAAAACGATAACATTATTGCTAGTCATATCGTTAAGGCGCTGAAGAACAAGGACACCAT
GCTGATCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101617 representing YP_073817
Red=Cloning sites Green=Tags

MSLSSLFNGKYDTKFLLNMSSAAKVELIVEKVAALADACLETPLPTDWFRNILDPELEFNSNFEEIHSIG
DEEFAQPLPFLPFRVLLITGTAGAGKTSSIQTLAANSDCLITATTSIAAQNLSGLLNRTKSAQVKTIFKT
FGFNSSHVSMNERISCSVTTLDSIADQQKHDLSTYWNVIADIAERALNAANGKAKVIPDLCESSVIVIDE
AGVILRHILHTVVFFYWFYNGLHKTQLYKNRVIPCIVCVGSPTQSGALISSFNPLTQNKDVKKGFDILSA
LICDDILSNYCKISENWVIFVNNKRCTDVEFGEFLKHIEFGLPLKPELIEYVDRFVRPATYIRNPTNEIG
MTRLFLSHYEVKSYFKVLHEQVELTNKDNLFTFPVYFIIQNKAFEDYKNEISNFTLEIEPWFKTNLHRLN
TYSQFADQDLSKTIQIEEIVLDDGSVEETLITCHLKHIKHSSIGVTSRTKSSTVGFSGTYEKFVELLQSD
LFIEKTACEYSVHAYSFLTGLMYGGMYSFCLSEFTTSEVMTEIRKIKLPNIDFLQTMTAEVSLQTFDESD
EYYDLHIAPTDEEMLASDPCPDPFFLKYKQLPLTNVLTFEEISYLYTVFKEIFISRFAILQRHSKEMFGK
SNLITYNRNNVSSKRCGEICSHVKSFYGMLTYAVPANNYTLEGYTYDNVIFLGTDKMLPPIIYKRGLPKI
VIKDEMGFISILDNNVSKLTDTVNGNSFHICTTIDYAIVSKVAMTVTKSQGLSIQRVALDFGNDPKNLKL
SSIYVGMSRVVDPNNLIMNLNPLRLNYENDNIIASHIVKALKNKDTMLIF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 2460 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073817
RefSeq ORF 2460 bp
Locus ID 3289535
MW 92.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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