shRNA慢病毒质粒

慢病毒元件盒包含三个主要功能元件, shRNA，嘌呤霉素筛选标记和tGFP报告基因

Lenti shRNA

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shRNA 慢病毒载体信息

pGFP-C-shLenti vector

  • 第三代慢病毒载体系统
  • 原核表达系统氯霉素抗性标记
  • 5’LTR 和 3’LTR之间有三个主要功能元件：可包装为慢病毒颗粒并转导到多种细胞系
    1. shRNA 表达原件位于U6启动子之后
    2. 哺乳动物系统嘌呤霉素筛选标记
    3. tGFP作为报告基因

The shRNA expression cassette

  • 29mer hairpin with a 7bp loop.
  • U6 driven expression
  • A Pol III termination sequence (TTTTTT) immediately downstream of the hairpin.
  • The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.

OriGene also has another Lentiviral shRNA vector, pRFP-CB-shLenti

pGFP-C-shLenti vector map

慢病毒相关产品

慢病毒质粒 慢病毒包装试剂盒 转染试剂 对照病毒颗粒 病毒浓缩试剂 慢病毒载体 滴度测试试剂盒 稳定剂

科研使用者对我们慢病毒产品是如何评价呢？:

"Lenti Vector with GFP and selection marker makes easy to generate single cell clones."

- A scientist from NIBMG

"Aml12 cells are very difficult to transfect using siRNAs. Hence, using OriGene's lenti-shRNA product helped tackle this issue."

- A scientist from The University of Queensland

"We as research labs always look for an efficient and reliable lentiviral products and OriGene has really done a great job for past several years for us. We are extremely satisfied with the product's efficacy of knockdown, literature provided and technical support when needed. On top of this the service was fast enough. I shall highly recommend researchers to go for OriGene. Thank you."

- A scientist from Moffitt Cancer Center

"Our lab tried to package our lentivirus using 293T cells but it was not that easy. OriGene's lentivirus provided us consistent results which made us have clear idea about the further research goals. The purchases from OriGene have been worth the price."

- A scientist from University of Arkansas for Medical Sciences

"I have tried lentivirus expressing shRNA and it was working to my expectation. I am very happy with the product."

- A scientist from Centre for stem cell research

"The ready-to-use lentivirus particles worked well in our hard-to-transduce cell lines."

- A scientist from University Hospital of Tuebingen, Children's Hospital

"Great quality, good knock-down. Super satisfied."

- A scientist from Cambridge University

"We used lentiviruses to silence Pmch gene and it worked beautifully."

- A scientist from USP
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