MicroRNA过表达质粒

MicroRNAs (miRNAs)是一种天然约21—23个碱基的单链非编码小分子RNA，在很多生物学过程及疾病发展过程中具有重要作用。miRNA形成过程如下：

DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA

OriGene提供您所需要的过表达的miRNA克隆，长度大约600bp，上游带有CMV启动子。在转染过程中，细胞内将以CMV启动子驱动miRNA前体表达为成熟miRNA，同时可以进行细胞内功能性分析。

  • 覆盖全基因组miRNA
    - 包含1829条人源、1164条小鼠和436大鼠的miRNA
  • GFP标记，便于观察转染效果
  • 新霉素作为稳定表达的筛选标记
  • 提供10微克转染级质粒，由PowerPrep® HP 试剂盒纯化，可即时转染
  • 可提供3'-UTR报告克隆 ，进行miRNA靶标验证
  • 载体: pCMV-MIR
MicroRNA Processing Diagram

 
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可订购套装产品

Catalog No. Description List
SC410001 Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes)
SC420001 Human miRNA expression plasmid set (646 vectors, 10ug each in 2-D bar coded tubes)
SC410002 Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates)
SC420002 Human miRNA expression plasmid set (646 vectors, 2ug each in 96-well plates)

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最新引用文献

A New Polymorphism Biomarker rs629367 Associated with Increased Risk and Poor Survival of Gastric Cancer in Chinese by Up-Regulated miRNA-let-7a Expression, Xu, Q;Dong, Q;He, C;Liu, W;Sun, L;Liu, J;Xing, C;Li, X;Wang, B;Yuan, Y;PLoS ONE e95249 9 4. April 2014 [MIR-let-7a SKU# SC400707]

Association of a peptoid ligand with the apical loop of pri-miR-21 inhibits cleavage by Drosha, Diaz, JP;Chirayil, R;Chirayil, S;Tom, M;Head, KJ;Luebke, KJ; RNA, Feb 2014. [miR-16 SKU# SC400796]

Hepatitis C Virus Induced Changes in miRNA-107 and miRNA-449a Modulate CCL2 by Targeting IL6 Receptor Complex in Hepatitis, Sarma, NJ;Tiriveedhi, V;Crippin, JS;Chapman, WC;Mohanakumar, T; J. Virol., Jan 2014. [MIR-107 SKU# SC400023]

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