U48 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101588

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein H [Human herpesvirus 7], codon optimized for human cell expression, YP_073788



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CNY 10,450.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101588 represents NCBI reference of YP_073788 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACTTTTACATTAACTCTCTGTTGCTCATCGTATCCATCAATGGTTGGAAGCACTGGAATATTCTTA
ATAGCAGCATCTGCGTTAACGAAAAAACTAACCAAACTATTATACAGCCAGGACTGATTACCTTTAATTT
TCACGACTATAATGAAACTAGAGTCTACCAGATCCCCAAGTGTCTGTTCGGTTATACATTTGTGTCCAAT
CTCTTCGACTCCGTGAATTTTGATGAAAGTTTTGATCAATACAAGCACCGCATCACAAGATTTTTTAACC
CCTCTACCGAGAAGGCCGTCAAGATTTACGCCCAAAAGTTCCAGACAAATATCAAGCCGGTCAGCCATAC
TAAAACTATAACGGTGAGTCTCCTCCCACTCTTTTATGAAAAGGATGTGTATTTTGCCAACGTGAGTGAG
ATCCGGAAACTCTACTATAACCAATACATCTGTACTCTGTCCAACGGCCTGACAGACTACCTCTTTCCCA
TTACTGAGCGCTGCGTTATGCGGCACTACAACTATCTGAACACCGTGTTTATGCTTGCCCTGACACCCTC
CTTCTTCATTATCAGTGTTGAGACAGGAATGGACGACGTAGTTTTTATCTTTGGCAACGTCTCACGGATC
TTTTTTAAAGCCCCCTTCCGGAAGTCAAGTTTCATTTATAGACAGACAGTGAGCGACGACCTCCTCCTCA
TAACAAAAAAGACCACCATTGAGCGCTTTTATCCATTTTTGAAGATTGATTTTCTCGATGACATCTGGAA
ACAAAACTACGATATCAGCTTCCTGATAGCTAAATTCAACAAGCTTGCTACAGTATATATCATGGAGGGC
TTTTGTGGAAAGCCCGTTAATAAGGATACATTCCACCTTATGTTCTTGTTTGGCCTGACCCACTTCCTGT
ACAGTACCCGCGGAGACGGCCTGCTGCCCCTGCTCGAGATCCTTAATACTCACCAGAGTATAATTACTAT
GGGAAGGTTTCTGGAGAAGTGCTTTAAGATGACCAAATCTCATCTTCTCTATCCTGAGATGGAAAAATTG
CAGAATTTCCAATTGGTCGACTACTCATACATTACTAGCGACCTCACAATCCCCATCTCCGCCAAACTCG
CGTTTCTGTCTCTTGCAGACGGTCGGATCGTGACCGTGCCACAGAATAAATGGAAAGAGATTGAAAATAA
CATAGAAACCCTGTACGAAAAACACAAACTGTTTACTAACCTCACCCAGCCCGAGAGAGCCAATCTGTTT
TTGCTCTCAGAGATTGGGAACTCTCTGGTATTCCAGGAAAAAATCAAAAGGAAAATTCACGTGTTGTTGG
CGTCTTTGTGCAACCCACTGGAGATGTATTTCTGGACCCACATGTTGGATAACGTGATGGACATCGAAAC
AATGTTCTCTCCATGTGCAACAGCGACAAGAAAGGACCTGACGCAGCGGGTCGTGAACAACATTCTCTCA
TACAAAAACCTCGACGCATACACTAATAAAGTGATGAACACTCTGAGCGTGTACCGAAAAAAACGACTGG
ACATGTTCAAATCAATCAGCTGCGTTTCAAATGAACAGGCTGCCTTTCTGACTCTGCCCAATATTACATA
TACAATCAGTTCTAAATACATCCTGGCAGGAACCTCTTTCTCTGTAACATCAACTGTGATTAGCACAACA
ATCATCATCACGGTCGTTCCCCTTAACAGTACTTGCACACCAACAAACTACAAGTATTCTGTGAAAAACA
TTAAGCCCATTTACAACATTTCCTCACACGATTGTGTCTTCTGCGAATCCTTGGTGGTGGAGTACGACGA
TATCGACGGCATTATTCAATTTGTTTACATTATGGACGACAAGCAGCTGTTGAAGCTCATCGATCCCGAC
ACAAACTTCATAGATGTCAACCCTAGAACACATTATTTGCTTTTCCTCCGCAACGGGTCCGTGTTTGAAA
TCACAGCTCTGGATCTCAAGTCTTCTCAAGTGTCCATTATGTTGGTCCTGCTTTATTTGATCATTATTAT
CATTGTGCTGTTCGGTATATACCATGTATTTAGGTTGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101588 representing YP_073788
Red=Cloning sites Green=Tags

MYFYINSLLLIVSINGWKHWNILNSSICVNEKTNQTIIQPGLITFNFHDYNETRVYQIPKCLFGYTFVSN
LFDSVNFDESFDQYKHRITRFFNPSTEKAVKIYAQKFQTNIKPVSHTKTITVSLLPLFYEKDVYFANVSE
IRKLYYNQYICTLSNGLTDYLFPITERCVMRHYNYLNTVFMLALTPSFFIISVETGMDDVVFIFGNVSRI
FFKAPFRKSSFIYRQTVSDDLLLITKKTTIERFYPFLKIDFLDDIWKQNYDISFLIAKFNKLATVYIMEG
FCGKPVNKDTFHLMFLFGLTHFLYSTRGDGLLPLLEILNTHQSIITMGRFLEKCFKMTKSHLLYPEMEKL
QNFQLVDYSYITSDLTIPISAKLAFLSLADGRIVTVPQNKWKEIENNIETLYEKHKLFTNLTQPERANLF
LLSEIGNSLVFQEKIKRKIHVLLASLCNPLEMYFWTHMLDNVMDIETMFSPCATATRKDLTQRVVNNILS
YKNLDAYTNKVMNTLSVYRKKRLDMFKSISCVSNEQAAFLTLPNITYTISSKYILAGTSFSVTSTVISTT
IIITVVPLNSTCTPTNYKYSVKNIKPIYNISSHDCVFCESLVVEYDDIDGIIQFVYIMDDKQLLKLIDPD
TNFIDVNPRTHYLLFLRNGSVFEITALDLKSSQVSIMLVLLYLIIIIIVLFGIYHVFRLF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 2070 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073788
RefSeq ORF 2070 bp
Locus ID 3289506
MW 80.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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