U95 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101626

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for protein U95 [Human herpesvirus 7], codon optimized for human cell expression, YP_073826



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CNY 14,160.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101626 represents NCBI reference of YP_073826 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATCCGGCGATTCCTTTGGCAACAATCACCAGGTGAGCAGCAATAGTGACGCATTCCAGTTCCGAC
AGTACTCAACCACCGTGGACACCTTCGCTTACTCTTCCATGGACCCAAGTAACTGTATGCTGAACGAACA
GACCCATCTTGAGACAGTGCTGGTCTACCCAGTATACAACATGTCTTCTCAGCAGACAGTGGAGCGGAAT
CTGTATGCGAATAATATCGGCAATGGGAATGTGCTGAGTTATTGCAGGCAGGATAGCCTGCAGGGTAATC
AGGGAACATATTTTCCAACGTATCAAGGGTTTCACTCCAACACACCACATAATTTTTCCATCGAGAATTT
CAACTTTGGGACTAACGTCATTAGACCCATTCCTTTTAGAAACGAAGGAGTCGATGCCTCCACATCAAAG
GATTACGATTTTAAGCCTTGCATCACCACTATGCAAATCCAATTCTCTCAGCCCTCTGCCTTCCAGACAT
ACTCTCTGATGAATGGAAACTTTAGCGAGAACGGCTACGGATACAATACCAAAATTGGGGAAAACTGCGC
AATGAGCTACGTGGAACAAGAGAGTGTCGACTCTAAATACCCTAATAAGGAAGTGTCAATCTCCTATCAA
CGCAACGAGCATTACGATGAAAATCTCCCTACCCGCGAATTGAATACCGACTTCCCCCAGTATAACCAGT
CCAGTGTTCTCCAGCACACGTTTGCTAATGTTCAGAGTATCATAAGCCAGCAAAGCCCTTATCAGCTCAT
CGGAAAAGAAAATAGCTTCAACAACTACCTTGAGACCGCTAATGTGGACAGCGACTGCCAGAGTAACGGC
GGGCCAAAGATTGGACTGGATACAGACCAGTCTGTTTTCTCCGATGAGGTGACGGGTGCCTGCGTTGAGA
ACGTGCATTTCCCCATGAACCCAAAGAACGAGTATGTCAATAATATTTCCCTCGATGTTCAGACAGAGTT
TCCCGTCACAGAGAATCCTCTGGTTGGCGAAAGTCAAGCAACTAAGAATAAGGATGTGGACAGCAATGCC
GAAGTGAATAATCACTCCAAGTACAGGCTGCTGAAACGAAATATAACTCCCACCATGGGCAATATCAAGC
ACCTTAACTTTAGTTGCAAAATCAGCACTGAGGAGGAGCGAAAAACCTTTTTCAACCGATTGAGCGAGTT
GCTGAAAATCAGAGATGACATTAAAAATACAAAAATCAGTAGTAAAGTGGACTTCATACAGAGTGAGGCC
TCAACCTCTTTGGACATATGTAAGAATACCTTTAACAACAACTCTGATAGCTCCGATGTGGACACAGATA
TTCTGGCAGATAACCCTCTGGTGATCTGTGAGAACGAGCTCATTTGCGATAACAACGAGGAGAATATTAA
GTTCCCCCCGAACGTGGAGAAAGAAGCCGTTCCTATGCAAACTGTGAAGAGGTCTTTCCCTGAAATCTGC
CCTGAGCATTTTAAGAAGCGCCGATTTATTAACGGCGATGTGATATACGAAGATTTGAACTCTGTGTATA
AGGTCATGCCCGCTAGCGCCACCTATGATGATGTCAGATTCGGGGAAGTTGACTATCAAACCTCATCCGC
TCAGACAAAAATCTCCAACCATCAGCTTGCTCTGCTGCCCACAAACTACCAGCACATGATCGGCCAGGAG
ACAGACATAAGTTCTAGGGATTACCACAACGACAGCGCCCAGATCATATACAACTGTTCCTTTCAAAGGC
AGGGTAAGAGACTTCTCGCTGACATCCCTTACAGGCCGTGGCTGAAAGAGAATGTCCCCACAGGCGAGTA
CGGAAAGAGGTTTGTGAAGACCGTGCCCCGCCCAGCCTCCGTATTTATTTTCGGTCGGTGCAGAAAAAAA
ATGCTGAGGGAGAGTGTTAAGTCTTTTATGCACTTGAGTAAGTTGCAGTATACCAAGGAAAGCTTGGAGG
CCTATGTCCTGAGAAACTGTAACAAGTTCCTGGATTTGTCATGGCCAATACGCCACAAGATCTATATCAT
GCCTGATGTCAGTCGAAATTATAACATTGAGGAAGTAAAGAACCTGTTCCCTGTCCCTGAAGGCTGGATG
GTGACTCTCGGGTTTGTGGGAACGGAGGAACCCGTGACAAAGATCTATAATATCGCCGTGCTTCTTTGCG
AGAACGGCTGGGTGATGATTCATAAAAACGATAAAGTGGAACCTGAGCTTTACCTCGCGGCATCAAACCT
GAACGAATTGATTGAAGATGGTCTGGCGCGCTGTGACTGCATTTATGAAAAGAGATCCGTCCCTTACGGT
ATCGTTATGGAGGGAAAACTGAGGCAGTTCATGGACAACTTTGGCTCCCTGCAGTCTGTTCTGGCATATC
GGAAGTACCTTCACGGGTTTCTGTGGGCCTTCAACGGGACCCCTGGGCGCCTGGCTGATAGGGTGTTTCA
CACGTGCGTGCCCGGCGTGCACAATGCCTTGCCCCTTGATGCAGTCATTAAGCATGAAAACAACCCGCTT
TACTTCATTGGCTACGTCACAACTTTTAAGCAGCAGAATGACTTTAATGCGAACGTGTTCATTGCCGTTG
ATGGGAACCTCTCAATATACGGCTATCACCTTATCTCCCAGAAGACATGGTTCCTGGCTAAAACTTTTTC
TACTTTTCTCAAGATGGGTACACGAAAGATGTATTATGACTACGAGATACCCCTTAAGATCCACCTCGGC
GATTCAGCGGATAGCTTCCTGAGCTGTTTCAAGAACGCACCCTGCCTGCTGCTGAAGCCCGAAGTCCTGC
GCAAACAATTCCCCAAGCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101626 representing YP_073826
Red=Cloning sites Green=Tags

MESGDSFGNNHQVSSNSDAFQFRQYSTTVDTFAYSSMDPSNCMLNEQTHLETVLVYPVYNMSSQQTVERN
LYANNIGNGNVLSYCRQDSLQGNQGTYFPTYQGFHSNTPHNFSIENFNFGTNVIRPIPFRNEGVDASTSK
DYDFKPCITTMQIQFSQPSAFQTYSLMNGNFSENGYGYNTKIGENCAMSYVEQESVDSKYPNKEVSISYQ
RNEHYDENLPTRELNTDFPQYNQSSVLQHTFANVQSIISQQSPYQLIGKENSFNNYLETANVDSDCQSNG
GPKIGLDTDQSVFSDEVTGACVENVHFPMNPKNEYVNNISLDVQTEFPVTENPLVGESQATKNKDVDSNA
EVNNHSKYRLLKRNITPTMGNIKHLNFSCKISTEEERKTFFNRLSELLKIRDDIKNTKISSKVDFIQSEA
STSLDICKNTFNNNSDSSDVDTDILADNPLVICENELICDNNEENIKFPPNVEKEAVPMQTVKRSFPEIC
PEHFKKRRFINGDVIYEDLNSVYKVMPASATYDDVRFGEVDYQTSSAQTKISNHQLALLPTNYQHMIGQE
TDISSRDYHNDSAQIIYNCSFQRQGKRLLADIPYRPWLKENVPTGEYGKRFVKTVPRPASVFIFGRCRKK
MLRESVKSFMHLSKLQYTKESLEAYVLRNCNKFLDLSWPIRHKIYIMPDVSRNYNIEEVKNLFPVPEGWM
VTLGFVGTEEPVTKIYNIAVLLCENGWVMIHKNDKVEPELYLAASNLNELIEDGLARCDCIYEKRSVPYG
IVMEGKLRQFMDNFGSLQSVLAYRKYLHGFLWAFNGTPGRLADRVFHTCVPGVHNALPLDAVIKHENNPL
YFIGYVTTFKQQNDFNANVFIAVDGNLSIYGYHLISQKTWFLAKTFSTFLKMGTRKMYYDYEIPLKIHLG
DSADSFLSCFKNAPCLLLKPEVLRKQFPKP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 2820 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073826
RefSeq ORF 2820 bp
Locus ID 3289544
MW 107.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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