U38 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101577

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 7], codon optimized for human cell expression, YP_073778



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CNY 15,390.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101577 represents NCBI reference of YP_073778 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTTGTTTCTTTCTTTAATCCTTATTTGGAAAACGTGAGAACCAAGAAGAAGACCAAGAGCACTT
TTCTGAGGATCTTCCCCAGGGGAATCATGCATGACGGAGCCCCTGGCCTGATGAAGACCCTTTGCGATTC
TGAGCCCAGAATGTTCTACCAGGACAAACAATACATACTGAAAAACGACATGACCTGGCCTTCCTTGTCC
CAGGTCGCAGAAAAGGAACTCCGGGCACCCTTGAAATTCCACATCTATGACGCATCTGAAAGTCTCTTGT
TTACCGATAGCATAGAGAATATTCCCTTCCAGTATAGGCATTTCGTCATCCCGAGCGGCAACGTCATAAA
GTTGTTTGGGAAAACCGAGTGCGGGAAGAAGGTATGCATCAATGTGTTCGGGCAGAACAGCTACTTCTAT
TGCGAATATCAGTGCAAAAAAGAGCTGAACAGCAGAATCTGCTCACTGCTTAATAGTTCCGAAATCAAAA
TGAGCTGTTCCTTTAGTATCGAGAGCGTAACTAAATACAACTTCTACGGCTATAATACCGAGCCAATCAA
GAATTTGTTTAAGCTCAGCTTTTCCAATTTCTACATCTCCAACCGCATTGGCAAAATCCTTCTCAACGAA
GGCGTGAGCGTGTACGAGGCAGAGGTGGAGATCCTGAACAGATTTTTTATTGATAACAACCTCAAGTCTT
TCGGCTGGTACCAGATAAACTATCTTTCAATCCAGGAGTTTGCCAAGTCTTCCAACGTAGAGATTGAGCT
CAATTGCCACGTATCCGATCTGTTCCTGCTGAAGGAGGATTCATGGCCTCTGTACGACTGTTGTAGCTTT
GATATTGAATGTCTTTCACAGAATGGCAACTTCCCCGACGCAGAGCAAATGGGAGATATAATCATCCAGA
TTTCTGTAATCGATTTCGATTCCGAGGGTAACTACCAGTCAAAACACCTCTTTACACTGGGCACATGTGA
ACAAATTGAGGGCGTGTTTATATATGAATTCGCCTCCGAGTTTGAACTCTTGTACGCTTTTTTCCTGTTT
TTGAAGTGCAAATCACCGGAAATCCTGACAGGGTATAATATCATCAATTTTGATCTCAAATACCTTTGCA
CTAGAATGGAAAAAATCTATAACTTCGAGATCGGAGGCTTTAGCAAGCTCAAGCGGGGCAAATTTTCCGT
CATCGTGCCATATGAACAGCACAAAAAATTTCTGAACTCCCTGACCAAGGTAAACATGTCAGGAATCCTG
TGCTTTGACATGTACAACGTTTACTCTTCTAAAATCAGCGCGCAGAACTATAAGCTGGATACGATTGCAA
AGCTGTGCCTCAATCAGGAGAAAAAAAATCTCAGCTACAAGGAGATCCCCAAAAAATTTATCGACGGCAG
TAAAGGCAGAGCAGTAGTCGGTCGGTACTGTATTCAGGACTCCTTGCTTGTGGTGCAGCTCTTCACCAAG
ATCAATTACCACTATGAAATGGCTGAAGTGGCTTCACTCGCATATATTACCATCAGGTGCGCAGTGTTTG
AGGGCCAGCAGAAAAAGATCTTTCCATGTATTCTTCATGAAGCCAAAAACCTGAATATGATATTGCCCTC
CATGAATACCAACTTCAATAAAGGGAAGGAGAACGTGGGCTATAAAGGCGCTACCGTACTGGAGCCCAAG
ATAGGCTACTATGCCACGCCTACAGTAGTCTTTGACTTTCAAAGCCTCTATCCAAGCATCATGATGGCTC
ACAATCTGTGTTATTCTACCCTGGTGGTGGATGAAAATGCCGTGATCGGGCTCCACGCAGATGACATTCT
CACAGTACACGTTGGACCGGTGACCCACCGATTTGTGAAAAAAACAGTGCGGGAAAGCATCCTCGCCTCA
CTGCTTAAGAAGTGGCTGGACAAGCGAAAGGAAGTGAAGATGCAGATGAAGATGTGTAATAACCCCGTCA
TGGAAATGCTTCTCGATAAAAAACAGCTGGCCCTGAAAACGACTTGTAACTCCGTGTATGGCGTGACAGG
CGCCACCCACTCTCTGCTTCCTTGCGTTGCCATCGCCGCTAGTGTTACCTGTCTCGGCAGAGAGATGTTG
TGCAAGACGGTCGACTACGTGGATTCCGCCATGTATAGCGACACATTTTTTATCGAGAAGTTCGGTCTGA
CCCGGGGTGACTTTTCAGGCACATTCGGAATCGAAGTCATCTACGGGGACACCGACTCCCTGTTTGTGAC
ATTCAAGAATGTATGCCCTAAAGCCCTGAAGAGGATCGCTCCTTCTATTGCAAGTCATATTACCAATACT
CTGTTTAAACACCCCATAAAATTGGAATTTGAAAAAATCCTGTTCCCACTGATTCTGATCTGCAAAAAAA
GATACATTGGGAAGCTTGACGACGCGACTCTGGTTTTTAAAGGTGTGGAACTCGTGCGGAAAACTAGTTG
CGATTTTGTCAAGGTTGTGGTCAAGGATATTATCGACTTGCTGTTTTGGGATGTTGATGTTCAGAAGAGT
GCCGAGAAGCTGTCCAACATGACAATACAGGAGATCTATGAAAATGGGGTGCCCGATGGAATACAGAAAA
TCATTAAAAAACTGTGTGATGCTAGGGATGCCCTGTTTCTGAATAGGGTGAACATCAAGAGCCTTGTGTT
GAGTAGCGTTCTCAGCAAGGATATTAGCGCTTACAAACAAGCCAACCTTCCCCACCTGGCTGTTGTTAAA
AGACTCGCCCAGAGAAAAGAAGAACTGCCCAATATCGGTGATCGCGTAATGTACATCCTTATCGCGCCAA
CCGAGACTGTGAAGAAAACGTTTCATAATTATGAGCTGGCCGAAGACCCTGCCTATGCTATAGAGAACAA
TCTGAAGATCAATGCTGACAAGTATTTCGAGCAGATAATGAAAGCCGTGACCAATGCCATCTCCCCTATC
TTCCCGAAAACCGGGATCAAGAAGGAAAAGTTCCTTCTTAGCATTCTGCCTCTGAAAGTGTATGTCGATC
AGAGCTTTTGCGACCTTACTGACGTGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101577 representing YP_073778
Red=Cloning sites Green=Tags

MDLVSFFNPYLENVRTKKKTKSTFLRIFPRGIMHDGAPGLMKTLCDSEPRMFYQDKQYILKNDMTWPSLS
QVAEKELRAPLKFHIYDASESLLFTDSIENIPFQYRHFVIPSGNVIKLFGKTECGKKVCINVFGQNSYFY
CEYQCKKELNSRICSLLNSSEIKMSCSFSIESVTKYNFYGYNTEPIKNLFKLSFSNFYISNRIGKILLNE
GVSVYEAEVEILNRFFIDNNLKSFGWYQINYLSIQEFAKSSNVEIELNCHVSDLFLLKEDSWPLYDCCSF
DIECLSQNGNFPDAEQMGDIIIQISVIDFDSEGNYQSKHLFTLGTCEQIEGVFIYEFASEFELLYAFFLF
LKCKSPEILTGYNIINFDLKYLCTRMEKIYNFEIGGFSKLKRGKFSVIVPYEQHKKFLNSLTKVNMSGIL
CFDMYNVYSSKISAQNYKLDTIAKLCLNQEKKNLSYKEIPKKFIDGSKGRAVVGRYCIQDSLLVVQLFTK
INYHYEMAEVASLAYITIRCAVFEGQQKKIFPCILHEAKNLNMILPSMNTNFNKGKENVGYKGATVLEPK
IGYYATPTVVFDFQSLYPSIMMAHNLCYSTLVVDENAVIGLHADDILTVHVGPVTHRFVKKTVRESILAS
LLKKWLDKRKEVKMQMKMCNNPVMEMLLDKKQLALKTTCNSVYGVTGATHSLLPCVAIAASVTCLGREML
CKTVDYVDSAMYSDTFFIEKFGLTRGDFSGTFGIEVIYGDTDSLFVTFKNVCPKALKRIAPSIASHITNT
LFKHPIKLEFEKILFPLILICKKRYIGKLDDATLVFKGVELVRKTSCDFVKVVVKDIIDLLFWDVDVQKS
AEKLSNMTIQEIYENGVPDGIQKIIKKLCDARDALFLNRVNIKSLVLSSVLSKDISAYKQANLPHLAVVK
RLAQRKEELPNIGDRVMYILIAPTETVKKTFHNYELAEDPAYAIENNLKINADKYFEQIMKAVTNAISPI
FPKTGIKKEKFLLSILPLKVYVDQSFCDLTDVL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 3039 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073778
RefSeq ORF 3039 bp
Locus ID 3289496
MW 115.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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