U41 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101580

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human herpesvirus 7], codon optimized for human cell expression, YP_073781



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CNY 17,100.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101580 represents NCBI reference of YP_073781 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATGACAACGAAACTGTGGTTAGTGCGCCAATATGCACTGCCGCTTGGCTCTACATCCTGCCCA
AGGAGCAGAAGCTCATTGAAATTCTGACTACGCTGTCCCTTATGGAAAAGCGCAAATCTGTGGTTATCTC
CCCATTGCTGTTGAACCTCACAGTGGAAAACGATTTCTTCCCCACCGTCAAGACCCCTATCATAAATTAT
GGCGGCACTGTGATCACCAAAATTACATCTTTCATGCCTGTCTGCTTTTTCTTTCATGGGACCGACGTGT
TCCTCAAAGAGGCGGAAGATCACGGAAATCTGGATAAACTCTGTAAACAGACCCGCGAAAAATTTAACCT
CCAAGAATTTGTTGTCAATGGCAATCGCAAACCCGTGGACATTGGTAAGATTTGCGAAAGCGTTGGCAGG
AATGCCGACGACGTCCTGTGCCATATTGTGGTGGGGAATGGTTTTAAGGAGCTGTTGTTCGCCGGTCTGC
TTATTCCCTGTGTAGAAGAGCAGATACAGGTGCAGGTAGGTGAGTGTCTGGCCATAAAGATACCACTTTA
TTCTGCAACGCTCTTCGAGTCCGAGGAGACGTTGTGCATTGACACATGCACAGAATTTATCCAGGAAAAT
GGGTTTTATGCCCCACAAATTAGCGAGGTTCTCTTCTATCTGATTTTCACCTCCTGGGGAATGACACTGC
GCTTCAATAATACTCTCGAGCTCATCAAGGCCGGGCTGAAGCAGTTCATTCAAGATACCGAGCAAACTGT
GAAACTTGCCCCCAACAAAACGTATCATGGTATACCAGGACAAAAGCTGTCCCCAATCGAGAAGGATCAC
CTCATGCTTGTTGATGCCGTTATCACAGAACTGACCTTTTCTTATACCGCCGAGTACCTGGATTCCATTT
ACGAAAATAACCAGATCATGAATTTTTCAGAGTGGCCCATCATTAAAAGTGCAGAAACCCACGAGGAGAA
AATCGTTGAGCTGAAAAAGCTTCGGCTTCACCTGTCATCCCATGTCGCCGCGCTCGTATTCGCAGCGAAT
AGCATTCTCTACTCAAATAAGCTGGCTTATATTAGTAACACTAAGCAGGCCTTCAATAGCGCCATTACCC
AGGAGACCCTGCTGCGATCAATTCAATTCTGCAATTCTCTGTCTAGCTTGAATGAAGATTTTTATAATGA
CGCCAGAAAGTTGATCAAATGCAACTCTAGTCCTTGCAAGGAGGATAAGTTCTCTGCATTCCACCTGGCC
TATGCCTGTGCAACTTGCCCCCAGATTCTGTCTCATATAATCTGGAATCTCAACAGAATGTCCATATACA
ACACAAATTGTGGGAACTCTGAAATTTACAACCATATAGTTAACTGCAGTAGCAATCTTTGCGAGTTCTG
TGAGGGCAAGTGCTGCCACTCTTGTATTGGCACGGCCCTGATCCGCATCAACAGTCGGCTCCCACAGATT
TCCAAGACCACGAAGAAGGAGCCAATCGTCATGACAATGTTTTCCCGGTTCTACGCTGATGTGGATGTGC
TGGGAAGTTTTGGGAAAAAGGGCGTTAATGAGTCAAAGGATCCCATGAAAGAAGCCCAAACCACACCGTC
ACTGGACCGATTTAAATTCCTCGGTATGATCCACGATTACTGCAAGAAAAATAACTTGATTGACGCGATT
ACTGGAGAGGATAACCTTAACTTTAAATCACAGAACGACTTCGTCAACATGATTAACGATCTTATCCAGT
GCATTGAAGAAGCGGTGTCAAAGTGTATTAGTGAGATGAGAAAGACCCAGACAAGTCGAGAGCAGATCGA
GAATTGCCTGCAGTCCTTCAATATCGACACAACCCCATTGTCCTTGGCTTTCTCCCCATTTTTCGTGTTT
ACGTATTATAAGGTGATTCTGATTGTTCTCCAAAACCTCGCTCTGATTATCGGCACTGGCTATGTGGTGG
ATCGACCCTGCACTGGCAACCTCATCAGCAAATGGCTTATGCAACAGTATCAGAGCCTCTACGGGGCATT
CTACAACAGTCACTTTAAAAAGGGCTTCCTCAATATGAAGACCGTCAAGATCGCCAGCAACGTGGACATG
GAACAGTACATCGACTTCAACCTGTTCAAGAGCGGGAAGTACGCAAAAACATCCATTCAGGCAAAACTGT
GCCGGCTGTCAATGCAATGCCTGAAAGACTTCCGCGTGAAGAATCGACCCTTTAACAAACCCAATAAGAA
TACCCAGAACAACCCCTTTTTCAAGAAAGTTAAGCAGAAGAAGAACCCCCTGAGTGGCTGCCTGTCTTTT
CTGCTTTTTAAATACCACGAGCGGCTTTTCCCTAACTTGAAGATCTCTTGTCTTGAATTTTGGCAGCGCA
TCCTGCTCAACAATATGCCGAAAACAATTGACATTGGGAATGTTGAGGACATGAGAAGTTTCATCAAGTT
CACGTTCAGAGTGACAAACAGCTACGATGAAATAGATCTGCTGGACATCCAGCCAGAGTGTTTGCTGAGT
TTTATTGAATACTATTTCCACAACAAGTTGCTTAGCGTTCTGGGGTATCGGGATTATCTCACGTCTCTGC
ACGCTTTGACATCAAAGCTCGTGCCCCAGAATCCCATGTTGTTCCCTGTGTTCCTGAAGGAACACCCCAC
CTTCTCTAGCGTGCAGGAATACGTTATGCACGTGAAAAAACTGGTTGGGAATGGACTGAAAGAGCCGATG
ACTGCGAGCCTTACGAAAGAGCCGAATTTCGGTTCCATTTTCACAGGCCGCTCCATAATAACGTTCGGCC
TGATGATTGAGAAATTTGTGTCTGTGGCATCAAGGGACTACTTCCACTTTGGACAGCTGGGGTGGATCGC
AGGGAGCGGTGTGGACAGGAACCTGAACCCCCCAAGCTCCGGTCTCCAAGACTTTCGCTTCATGCGGCAG
AAGTTTGTGATCGCCACAAAGCTTTGTGATATAATCGTGAAAAAAGTGAAACGGGAAGCAATCGTGTATG
ATGTGGAAGTCATCCGCGGGAAGGTGCTCAATATTATAGAGAGTCTCAGTAACTCCGTGAATCCGGAACT
GCTGATTCTCGCCGAAGTGATGAAGGATAGAGATTCAAAGCCAACTATGGACGATATGCTCTTCTATGTC
GACGGGCGCGAACCACTTGCGAAGTCTGTGATGAACAAGATCCAACACTTGACAGACTTGAATGTTCACG
ATTTCTCCCTTAGCACCTTGCTGTCAGTGTTCGAGGAGCAAGTCGAAGATAGCGCAGCCATTTACGACTT
CTCTGAGCTCCTGGTCGAAGGCAATGAACAGGGCTTTGGCATTCTGAAGTGCGAGGAGACTGAACACGAG
AACGAAGAGCCTAGTCTGAAGAAGGCACGGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101580 representing YP_073781
Red=Cloning sites Green=Tags

MADDNETVVSAPICTAAWLYILPKEQKLIEILTTLSLMEKRKSVVISPLLLNLTVENDFFPTVKTPIINY
GGTVITKITSFMPVCFFFHGTDVFLKEAEDHGNLDKLCKQTREKFNLQEFVVNGNRKPVDIGKICESVGR
NADDVLCHIVVGNGFKELLFAGLLIPCVEEQIQVQVGECLAIKIPLYSATLFESEETLCIDTCTEFIQEN
GFYAPQISEVLFYLIFTSWGMTLRFNNTLELIKAGLKQFIQDTEQTVKLAPNKTYHGIPGQKLSPIEKDH
LMLVDAVITELTFSYTAEYLDSIYENNQIMNFSEWPIIKSAETHEEKIVELKKLRLHLSSHVAALVFAAN
SILYSNKLAYISNTKQAFNSAITQETLLRSIQFCNSLSSLNEDFYNDARKLIKCNSSPCKEDKFSAFHLA
YACATCPQILSHIIWNLNRMSIYNTNCGNSEIYNHIVNCSSNLCEFCEGKCCHSCIGTALIRINSRLPQI
SKTTKKEPIVMTMFSRFYADVDVLGSFGKKGVNESKDPMKEAQTTPSLDRFKFLGMIHDYCKKNNLIDAI
TGEDNLNFKSQNDFVNMINDLIQCIEEAVSKCISEMRKTQTSREQIENCLQSFNIDTTPLSLAFSPFFVF
TYYKVILIVLQNLALIIGTGYVVDRPCTGNLISKWLMQQYQSLYGAFYNSHFKKGFLNMKTVKIASNVDM
EQYIDFNLFKSGKYAKTSIQAKLCRLSMQCLKDFRVKNRPFNKPNKNTQNNPFFKKVKQKKNPLSGCLSF
LLFKYHERLFPNLKISCLEFWQRILLNNMPKTIDIGNVEDMRSFIKFTFRVTNSYDEIDLLDIQPECLLS
FIEYYFHNKLLSVLGYRDYLTSLHALTSKLVPQNPMLFPVFLKEHPTFSSVQEYVMHVKKLVGNGLKEPM
TASLTKEPNFGSIFTGRSIITFGLMIEKFVSVASRDYFHFGQLGWIAGSGVDRNLNPPSSGLQDFRFMRQ
KFVIATKLCDIIVKKVKREAIVYDVEVIRGKVLNIIESLSNSVNPELLILAEVMKDRDSKPTMDDMLFYV
DGREPLAKSVMNKIQHLTDLNVHDFSLSTLLSVFEEQVEDSAAIYDFSELLVEGNEQGFGILKCEETEHE
NEEPSLKKARL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001716
ORF Size 3393 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001716.2, YP_073781
RefSeq ORF 3393 bp
Locus ID 3289499
MW 129.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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