U100 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101531

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for spliced envelope glycoprotein [Human herpesvirus 6], codon optimized for human cell expression, NP_050271



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CNY 7,410.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101531 represents NCBI reference of NP_050271 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCCGCCGAGACGGAGCGCTCCAATTCTTGTGTGCGCGATCTCTATGGCCACAGCCCTGAGCAATG
CGACCGTTCACCGAGACGCTGGTACTGTAGAGAGCACCCCTCCGCCTGATGACGAGGATAACTATACAGC
TAAATACTACGATGACTCTATTTACTTTAACATTTACGATGGAACAAACCCCACCCCAAGGCGGCGGACG
TTGCCGGAGATCATAAGTAAGTTTAGTACTTCCGAGATGTCCCGACTGGGAGGCCTCAAAGCTTTTGTGC
CCGTAGATTATACCCCCACTACAACACTGGAGGATATAGAGGACCTGCTCAATTATGCTATCTGTGACGA
TAATAGTTGTGGGTGTCTTATTGAAACAGAGGCAAGGATCATGTTCGGGGACATCATTATTTGTGTACCA
CTTTCAGCAGAATCCCGGGGGGTGCGCAACCTTAAATCACGCATTATGCCTATGGGTCTCTCTCAAATCC
TGTCCAGCGGGCTCGGACTGCATTTTAGCCTCCTCTATGGAGCCTTCGGCAGCAACTACAACAGCTTGGC
TTACATGGAGCGCCTGAAACCTCTTACCGCAATGACCGCAATTGCATTCTGCCCAATGACCTCAAAACTG
GAGCTCAGGCAGAATTACCGCCTCGAGAAGGCACGATGTGAACTGATCGTGAATATCGAGCTGCTGAAAA
TCCAAAACCACGGGGGACAGACCATAAAGACACTCACCTCCTTTGCAATCGTTCGAAAGGACTCCGATGG
CCAGGACTGGGAGACATGTACACGCTTCGCATCCGTTAGTATCGAGGATATTCTGAGAAGCAAACCGGCC
GCGAACGGAACCTGCTGCCCACCTCGCGATGTGCATCATGACAGACCTACCTTGCAGTCCTCAAATTCCT
GGACCAGGACCGAGTATTTTGAACCCTGGCAGGATGTGGTGGACGCCTACGTACCCATCAATGATAATCA
TTGCCCTAATGATAGCTACGTAGTGTTTCAGACACTTCAGGGCCATGAGTGGTGTTCAAGGCTTAACAAG
AACGATACTAAGAACTACCTCTCCAGTGTGCTTGCTTTCAAAAATGCTCTGTACGAGACAGAGGAGCTGA
TGGAAACCATCGGGATGCGATTGGCTTCCCAGATACTTTCATTGGTGGGTCAGCGGGGGACCTCTATACG
GAATATTGACCCTGCCATTGTGTCCGCCCTGTGGCATAGTTTGCCGGAGAAGTTGACCACTACTAACATT
AAGTACGACATTGCTAGTCCGACTCACATGAGCCCTGCACTGTGCACAATTTTCATCCAGACAGGCACCT
CAAAGCAGAGATTCAGAAACGCCGGTCTTCTGATGGTCAATAATATATTTACCGTCCAAGCCAGATACAG
CAAGCAGAACATGTTCGAAAAAAAGATCTATGGATACGAGCACCTGGGACAGGCACTTTGTGAAGATGGA
GAGATTCTGTTCCAGAATGCAGGACAAAAATTCTGTAGACCATTTACAGATAACAGAACAATCGTCTATA
CAATGCAGGATCAGGTTCAGCGGCCCTGGTCCGTAACATGGATGGATTTTAATCTGGTGATATCCGATTA
CGGCCGAGCCGTGATAGAAAATCTGACAGAATCAGCTATGAGCGCACATAAAAATGGACCAAGGTATCTG
CAGATGGAGACCTTCATCAGCGACCTCTTTCGATACGAGTGCCATCGCGACAACAGGTACGTACTCGAGA
AGAAACTTCAGATGTTCTACCCTACCACGCACATGAATGAGCTGCTGTTTTACCCTAGTGACCCCACTCT
GCCCTCACCATACGGTAATGGACACTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101531 representing NP_050271
Red=Cloning sites Green=Tags

MRPPRRSAPILVCAISMATALSNATVHRDAGTVESTPPPDDEDNYTAKYYDDSIYFNIYDGTNPTPRRRT
LPEIISKFSTSEMSRLGGLKAFVPVDYTPTTTLEDIEDLLNYAICDDNSCGCLIETEARIMFGDIIICVP
LSAESRGVRNLKSRIMPMGLSQILSSGLGLHFSLLYGAFGSNYNSLAYMERLKPLTAMTAIAFCPMTSKL
ELRQNYRLEKARCELIVNIELLKIQNHGGQTIKTLTSFAIVRKDSDGQDWETCTRFASVSIEDILRSKPA
ANGTCCPPRDVHHDRPTLQSSNSWTRTEYFEPWQDVVDAYVPINDNHCPNDSYVVFQTLQGHEWCSRLNK
NDTKNYLSSVLAFKNALYETEELMETIGMRLASQILSLVGQRGTSIRNIDPAIVSALWHSLPEKLTTTNI
KYDIASPTHMSPALCTIFIQTGTSKQRFRNAGLLMVNNIFTVQARYSKQNMFEKKIYGYEHLGQALCEDG
EILFQNAGQKFCRPFTDNRTIVYTMQDQVQRPWSVTWMDFNLVISDYGRAVIENLTESAMSAHKNGPRYL
QMETFISDLFRYECHRDNRYVLEKKLQMFYPTTHMNELLFYPSDPTLPSPYGNGHY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 1848 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050271
RefSeq ORF 1848 bp
Locus ID 1497092
MW 70.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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