U57 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101498

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050238



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CNY 20,330.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101498 represents NCBI reference of NP_050238 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAATTGGCAAGCCACCGAAATCCTCCCAAAAATCGAAGCACCCCTCAATATTTTTAACGATATTA
AGACCTACACTGCTGAGCAGCTGTTCGACAATCTTCGCATATATTTCGGCGACGATCCCAGCCGGTATAA
CATTAGCTTCGAAGCACTTCTTGGCATCTACTGCAACAAGATCGAGTGGATAAACTTTTTCACAACCCCC
ATCGCAGTCGCCGCAAACGTGATCAGATTTAACGACGTCTCACGAATGACCCTGGGAAAGGTTTTGTTCT
TCATTCAGCTCCCGCGCGTGGCCACCGGTAATGATGTAACAGCACCCAAGGAAACCACCATTATGGTAGC
CAAGCACAGTGAGAAGCACCCTATCAATATATCTTTTGATTTGAGCGCCGCGTGTCTTGAACATTTGGAG
AATACTTTTAAAAATACAGTGATCGACCAAATCCTGAACATCAACGCCCTGCATACAGTGCTTAGGTCTC
TGAAAAATTCCGCTGACAGTCTCGAGAGAGGACTCATACACGCATTCATGCAGACACTGCTGCGGAAGAG
CCCCCCGCAATTCATAGTATTGACCATGAACGAGAACAAAGTTCACAATAAGCAAGCCCTCTCCAGGGTT
CAGCGGTCCAATATGTTCCAGAGCCTGAAGAATCGGCTCCTGACTAGTCTGTTTTTCTTGAACAGAAATA
ACAACTCTTCTTACATTTATAGAATTTTGAACGATATGATGGAGTCAGTCACTGAGTCCATTTTGAACGA
TACTAATAACTACACCTCCAAAGAGAATATTCCTCTTGATGGCGTGCTGCTCGGACCAATTGGCAGTATC
CAGAAGCTCACCAACATACTCTCCCAGTATATTTCCACCCAGGTGGTCTCTGCCCCAATTTCTTATGGGC
ACTTTATAATGGGCAAAGAGAACGCAGTCACTGCTATCGCTTACCGCGCTATTATGGCCGATTTCACCCA
GTTCACCGTCAACGCCGGAACAGAGCAGCAGGATACCAACAATAAAAGTGAGATCTTCGATAAAAGCCGG
GCATACGCTGACTTGAAGCTTAATACATTGAAGCTGGGGGACAAACTGGTGGCATTTGATCACCTTCACA
AGGTGTATAAAAATACTGACGTTAATGACCCTCTGGAGCAGTCCCTGCAGCTGACTTTTTTCTTCCCCCT
CGGGATTTATATTCCTACGGAAACTGGCTTTAGTACAATGGAAACTAGAGTGAAGCTCAACGACACAATG
GAAAACAATCTTCCTACCAGCGTGTTCTTCCACAACAAAGATCAGGTGGTGCAGAGAATTGATTTCGCTG
ACATACTTCCGTCCGTATGCCATCCAATTGTTCACGACTCCACCATTGTGGAACGACTGATGAAAAACGA
GCCACTGCCTACTGGACATCGGTTTAGTCAGCTCTGTCAGCTGAAGATCACTAGGGAGAATCCAACGCGG
ATATTGCAGACCCTCTATAACCTGTACGAATCAAGACAAGAGGTCCCGAAGAATACAAATGTCTTGAAGA
ACGAGCTGAACGTGGAGGATTTCTACAAACCCGACAACCCAACTCTCCCCACCGAACGCCATCCGTTTTT
TGATCTCACCTATATCCAGAAGAACCGGGCCACCGAAGTGCTCTGTACCCCGCGCATAATGATCGGTAAC
ATGCCACTTCCTCTGGCTCCGATTTCTTTTCATGAGGCCCGAACCAATCAGATGCTGGAGCATGCTAAAA
CTAACAGCCATAATTATGATTTTACACTTAAAATCGTTACAGAGTCTCTGACTTCCGGGTCATATCCGGA
ATTGGCATACGTGATCGAAATTCTGGTCCATGGCAACAAACACGCCTTCATGATCTTGAAACAGGTGATT
AGCCAGTGCATATCTTATTGGTTCAACATGAAGCATATCCTTCTGTTCTGCAACAGTTTCGAGATGATAA
TGCTTATTTCCAATCACATGGGGGACGAACTGATACCAGGGGCCGCCTTCGCCCACTACAGGAATCTGGT
GAGTCTTATTAGACTGGTGAAACGCACCATCAGTATAAGTAACATCAATGAGCAGCTTTGTGGTGAGCCT
TTGGTAAATTTCGCCAACGCCCTCTTTGACGGCCGCCTCTTCTGTCCCTTCGTCCATACAATGCCAAGAA
ACGACACCAATGCCAAAATTACCGCGGACGATACCCCTCTGACGCAGAATACCGTCAGGGTCCGGAACTA
CGAGATCTCAGACGTGCAGCGGATGAACTTGATAGACAGCTCCGTGGTGTTTACAGACAATGACAGGCCT
TCTAATGAGAACACTATACTCAGCAAAATTTTCTATTTCTGTGTCCTGCCGGCTCTGAGCAATAATAAGG
CCTGCGGGGCCGGCGTGAATGTGAAGGAGCTTGTGCTGGATCTGTTCTACACCGAACCCTTTATATGTCC
TGACGACTGTTTCCAGGAAAATCCTATCAGTAGCGACGTTCTCATGAGCCTCATCCGCGAAGCGATGGGT
CCGGGCTACACGGTCGCGAATACATCCTCCATTGCTAAACAACTGTTCAAAAGCCTGATCTATATCAATG
AAAACACAAAAATTCTCGAAGTCGAAGTCTCTCTCGACCCGGCTCAAAGACACGGCAACAGCGTGCACTT
TCAGTCACTGCAGCACATCCTGTATAACGGGCTGTGTCTCATCTCTCCTATCACCACACTCAGAAGATAC
TATCAGCCCATCCCCTTCCACCGCTTTTTCTCCGATCCAGGAATCTGTGGTACGATGAATGCCGACATTC
AGGTGTTCCTCAACACCTTTCCACACTACCAGAGGAACGACGGTGGCTTTCCACTGCCACCTCCACTGGC
ACTTGAGTTTTATAATTGGCAGAGGACACCCTTTTCTGTGTACAGTGCCTTCTGTCCGAACAGCCTGCTT
TCTATTATGACCTTGGCAGCTATGCACTCAAAACTGAGCCCTGTCGCCATAGCTATTCAGTCTAAATCAA
AAATTCACCCCGGGTTCGCAGCTACCCTCGTGCGGACTGACAATTTTGACGTAGAATGCCTCCTGTACAG
CAGCAGGGCAGCAACGTCTATAATCCTCGACGATCCAACCGTGACCGCAGAAGCCAAGGATATCGTAACA
ACTTATAATTTCACCCAGCATCTGTCATTCGTTGATATGGGGCTCGGGTTCAGTTCAACCACAGCTACTG
CTAACCTTAAACGCATCAAGTCCGATATGGGGAGCAAGATCCAGAACCTCTTCTCCGCATTCCCCATCCA
TGCCTTCACCAATACGGACATCAATACCTGGATACGACACCATGTGGGCATCGAAAAACCAAACCCCAGT
GAAGGGGAAGCACTTAATATCATCACGTTTGGGGGGATTAATAAGAATCCTCCATCTATCCTGCTGCATG
GCCAACAGGCCATTTGCGAAGTAATCCTTACACCTGTTACCACCAATATCAACTTCTTCAAGCTGCCGCA
CAACCCACGCGGCAGAGAGTCTTGTATGATGGGTACCGATCCTCACAACGAGGAGGCCGCCCGCAAGGCC
CTGTATGATCATACCCAGACCGACAGCGACACCTTCGCGGCCACGACCAATCCATGGGCCTCTCTGCCCG
GGTCTCTGGGAGACATTTTGTATAACACGGCTCACCGAGAGCAGCTGTGTTACAACCCGAAAACGTACTC
CCCCAACGCCCAATTCTTTACGGAGTCTGATATTCTGAAGACCAATAAAATGATGTACAAAGTAATAAAC
GAATACTGCATGAAGTCCAACAGTTGTCTGAACTCCGACTCCGAGATCCAGTACTCATGCAGCGAGGGCA
CAGACTCCTTTGTCTCCCGGCCCTGTCAATTTCTGCAAAATGCCCTCCCACTGCACTGCTCAAGCAATCA
AGCCCTGCTCGAATCTAGGTCAAAAACCGGCAACACACAAATTAGCGAGACACACTACTGTAACTACGCC
ATCGGCGAAACGATCCCCCTTCAGCTGATCATAGAATCATCAATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101498 representing NP_050238
Red=Cloning sites Green=Tags

MENWQATEILPKIEAPLNIFNDIKTYTAEQLFDNLRIYFGDDPSRYNISFEALLGIYCNKIEWINFFTTP
IAVAANVIRFNDVSRMTLGKVLFFIQLPRVATGNDVTAPKETTIMVAKHSEKHPINISFDLSAACLEHLE
NTFKNTVIDQILNINALHTVLRSLKNSADSLERGLIHAFMQTLLRKSPPQFIVLTMNENKVHNKQALSRV
QRSNMFQSLKNRLLTSLFFLNRNNNSSYIYRILNDMMESVTESILNDTNNYTSKENIPLDGVLLGPIGSI
QKLTNILSQYISTQVVSAPISYGHFIMGKENAVTAIAYRAIMADFTQFTVNAGTEQQDTNNKSEIFDKSR
AYADLKLNTLKLGDKLVAFDHLHKVYKNTDVNDPLEQSLQLTFFFPLGIYIPTETGFSTMETRVKLNDTM
ENNLPTSVFFHNKDQVVQRIDFADILPSVCHPIVHDSTIVERLMKNEPLPTGHRFSQLCQLKITRENPTR
ILQTLYNLYESRQEVPKNTNVLKNELNVEDFYKPDNPTLPTERHPFFDLTYIQKNRATEVLCTPRIMIGN
MPLPLAPISFHEARTNQMLEHAKTNSHNYDFTLKIVTESLTSGSYPELAYVIEILVHGNKHAFMILKQVI
SQCISYWFNMKHILLFCNSFEMIMLISNHMGDELIPGAAFAHYRNLVSLIRLVKRTISISNINEQLCGEP
LVNFANALFDGRLFCPFVHTMPRNDTNAKITADDTPLTQNTVRVRNYEISDVQRMNLIDSSVVFTDNDRP
SNENTILSKIFYFCVLPALSNNKACGAGVNVKELVLDLFYTEPFICPDDCFQENPISSDVLMSLIREAMG
PGYTVANTSSIAKQLFKSLIYINENTKILEVEVSLDPAQRHGNSVHFQSLQHILYNGLCLISPITTLRRY
YQPIPFHRFFSDPGICGTMNADIQVFLNTFPHYQRNDGGFPLPPPLALEFYNWQRTPFSVYSAFCPNSLL
SIMTLAAMHSKLSPVAIAIQSKSKIHPGFAATLVRTDNFDVECLLYSSRAATSIILDDPTVTAEAKDIVT
TYNFTQHLSFVDMGLGFSSTTATANLKRIKSDMGSKIQNLFSAFPIHAFTNTDINTWIRHHVGIEKPNPS
EGEALNIITFGGINKNPPSILLHGQQAICEVILTPVTTNINFFKLPHNPRGRESCMMGTDPHNEEAARKA
LYDHTQTDSDTFAATTNPWASLPGSLGDILYNTAHREQLCYNPKTYSPNAQFFTESDILKTNKMMYKVIN
EYCMKSNSCLNSDSEIQYSCSEGTDSFVSRPCQFLQNALPLHCSSNQALLESRSKTGNTQISETHYCNYA
IGETIPLQLIIESSI

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 4035 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050238
RefSeq ORF 4035 bp
Locus ID 1497059
MW 152.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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