U77 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101516

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for Helicase/primase complex [Human herpesvirus 6], codon optimized for human cell expression, NP_050256



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CNY 12,540.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101516 represents NCBI reference of NP_050256 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTATTAGTAGCCTTTTCGGAGGTCGGTACGACAATAAGTTCCTCTTGAACATGTCATCTGCCCCAA
AGATAGAACTGATCGTCGACAAGGTGGCATCCCTGTCCGAAAGACGGCTCGAAGGCCGGCTCCCAGAGGA
CTGGTTTCGCCACATCATGGACCCAGAGACAGAGTTCAATGGTGAATTCGCCGACGCCCTGTGCATTGGA
ATTGACGAATTCGCGCAACCCCTGCCCTTCCTGCCTTTCAAAGCTCTGCTGGTCACTGGGACCGCAGGCG
CGGGGAAAACCAACTCTATACAGACTCTGGCCGCAAATTTGGACTGCATAGTGACAGCTACCACCTCAAT
TGCGGCCCAGAACTTGTCTGTGGTGCTGAATAGATCCAAGTCCGCCCAGGTGAAAACCATCTTCAAGACG
TTCGGGTTCAACTCAAGCCACGTGTCTATGTCTGAAAGGCAATCCTACATAGCCAATGACGAGAGGTCCA
TCCAAATTCAACAGAAGCAGGACCTGAGTATATATTGGAATGTTATAAGTGACATCGCCGAGCGAGCCCT
TGGTGCCGTGGCATGCAAGACCAAAGAACTGCCAGATTTGTGCGAGTCAAGCGTGATAGTTATAGATGAA
GCAGGCGTGATACTCAGACACATTCTTCATACCGTCGTATTCTTTTATTGGTTTTACAATGCATTGTACA
AGACTCCTTTGTACGAGAACGGCATTGTTCCTTGCATTGTGTGTGTAGGGAGTCCGACTCAGTCCAATGC
ACTCGTGACTTCTTTCAACCCACTTACACAGAATAAGGATGTCAAGCGGGGCATTGACGTCCTGAGTGCA
CTGATCTGTGATGACGTGCTGTCTAAGTATTGCGAAGTTGATAATAACTGGATAATCTTTGTCAATAACA
AGAGATGCGCAGATCACGCCTTCGGGGACTTTCTGAAGCACATAGAATTCGGACTGCCACTTAAGCCCGA
ACTGATCGAATATGTTGATCAATTTGTGAAGCCCGCATCATACATTCGCAACCCAATGAACGAGATTGAA
ACAACCCGGCTGTTTCTGTCCCACAACGAAGTTAAAAACTACTTTAGGAGCCTCCACGAACAGGTCGAAG
TGACTAATCGGAATAACCTGTTTGTCTTTCCAGTATACTTTCTGATCAAGAATAAGACATTCGAAGACTA
TAAATCAGAAATCGGTAACTTTAGCCTGGAAATTGAGCCATGGTTCAAGAGCAACATCCACCGGCTTAAC
ACTTACTCTCAATTCGCAGACCAGGATCTGTCTAAGACAGTTCAACTTGAAGAGATCGTGCTTGAGGATG
GGTCCGTGGAAGAAACCCTTATCACATGTCATCTCAAGCACATTCGGAATAGTTCCATTGGAGTGACATC
TAAGATAAAAGCCTCAACCGTCGGGTTTAGCGGAACTTATGAAAAGTTCGTGGAGCTGCTGCAAAGCGAT
CTCTTTATTGAGAAGACCTCATGCGAGCAAACCATTCACGCCTACAGTTTTCTGAGCGGCCTCATGTTTG
GCGGCATGTACAGCTTTTGTTGTAGCGAGTTCACTACGCCGGAAGTGCTGATGGAAATAAAAAATATCAA
AATGCCATCCATCGAATTTCTCGAGTCCGAGATGTCCAGGATGAGTAGGGACGTACAAACAGTGGAGACA
GATGAGCGGTATGACTTTGGCCTTGTGGATGATGGCCTGTCTGATATGGATCTCCTGGAGATCGATCCAT
GTGGCGATCCGTTTTTTACTCGGTACTCTAAGTTGCCGCTCACTAACTCCCTTAGCTTCGAGGAAATTAG
CCTGCTGTACACCACCTTCAAAGATATCTTCATCAGTCGCTTTGCTATCCTGCAAAAACATACTAAGGGG
AAATTTGGAAAAACCCTTCTGGTGACCTACAATCGCAATAACGTTTCCCGCAAACAGTGCGGCGAAATCT
ATTCACATCTGAAGAGTTTTTACGGCATGTTGACATATGCCATCCCCGCGAATAACTATACCCTTGAGGG
CTATACAAACGATAACGTGGTTCACCTGGGAACGGACAAACAGCTCCCGCAGATTCTGTACAAGAAAGGT
CTGCCTCGCTTGGTTATCAAGGACGAAATGGGTTTTATTTCAGTTCTGGACAACAACGTGTCCAAATTTG
TAGACGTGGTGAATGGGCAGTCTTTCCACCTGTGCACGACAGTGGACTACGCCACCGTGTCTAAAGTGAG
TATGACCATCACTAAAAGCCAGGGCCTGAGTATCCAGAAAGTAGCTATTGACTTTGGTTCTGATCCAAAG
AATCTGAAGCTCTCCTCTATCTATGTCGGTATGTCAAGAGTCACAGACCCAAATAATCTGATTATGAACG
TGAACCCTCTCAGATTGAACTACGAGAACGACAATTTTATCGCCCCACATATTGTGAAAGCTCTCAAAAA
CGAAAATACTATGCTCATCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101516 representing NP_050256
Red=Cloning sites Green=Tags

MSISSLFGGRYDNKFLLNMSSAPKIELIVDKVASLSERRLEGRLPEDWFRHIMDPETEFNGEFADALCIG
IDEFAQPLPFLPFKALLVTGTAGAGKTNSIQTLAANLDCIVTATTSIAAQNLSVVLNRSKSAQVKTIFKT
FGFNSSHVSMSERQSYIANDERSIQIQQKQDLSIYWNVISDIAERALGAVACKTKELPDLCESSVIVIDE
AGVILRHILHTVVFFYWFYNALYKTPLYENGIVPCIVCVGSPTQSNALVTSFNPLTQNKDVKRGIDVLSA
LICDDVLSKYCEVDNNWIIFVNNKRCADHAFGDFLKHIEFGLPLKPELIEYVDQFVKPASYIRNPMNEIE
TTRLFLSHNEVKNYFRSLHEQVEVTNRNNLFVFPVYFLIKNKTFEDYKSEIGNFSLEIEPWFKSNIHRLN
TYSQFADQDLSKTVQLEEIVLEDGSVEETLITCHLKHIRNSSIGVTSKIKASTVGFSGTYEKFVELLQSD
LFIEKTSCEQTIHAYSFLSGLMFGGMYSFCCSEFTTPEVLMEIKNIKMPSIEFLESEMSRMSRDVQTVET
DERYDFGLVDDGLSDMDLLEIDPCGDPFFTRYSKLPLTNSLSFEEISLLYTTFKDIFISRFAILQKHTKG
KFGKTLLVTYNRNNVSRKQCGEIYSHLKSFYGMLTYAIPANNYTLEGYTNDNVVHLGTDKQLPQILYKKG
LPRLVIKDEMGFISVLDNNVSKFVDVVNGQSFHLCTTVDYATVSKVSMTITKSQGLSIQKVAIDFGSDPK
NLKLSSIYVGMSRVTDPNNLIMNVNPLRLNYENDNFIAPHIVKALKNENTMLIF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 2472 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050256
RefSeq ORF 2472 bp
Locus ID 1497077
MW 93.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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