U43 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101484

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase/primase complex [Human herpesvirus 6], codon optimized for human cell expression, NP_050224



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CNY 13,020.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101484 represents NCBI reference of NP_050224 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTATAGTGGTTTTTGCGACCGAGTACGACGCTGCCAATGTGATCTTCTCCATTTTGTGCAGATCCC
CATCCGAGCACTTGATTTTCCCAATTATCGTCAAGTACAAGCCTAGCAACAATGTGAGCTTCTGCCTGCA
GACCCAGAAGTGCAAAAATTCCAAGCGGATTGATACAGTTTTCGTCTGCCACGCAGAGAAACTGAACCTC
TCACACTACATCCAGACCGCATCCCCCATAAAGGCCGAGGACGTGGCCAATTCACTGAATGACAAGGAGA
CAGAACTTCTGTATGTCGACATGATTTTGTCACAGACCGGGAAAGAGAAAGAAGATGTGGAGTTCAAGTA
CATGGCCTACTTTCACAAAAGCCTGATCATTAAATATCTCACAGGTAAGTTCCTTCTGCCTACTTCTCCA
TTTTGGTTCCTGAGCACTTATGGACAAACGGAAGGCCTGCTGTTGTTGACTATGTATTACTACCTGTTCG
AAGAGCAAAAATCCACGATAACCACCACTAAAAATTACGTCCAGTGTTTTACAGAGAACACCGGCAGCAT
GGTGTTCACCTACTCCTCAATGTCAGAATTTATTAATATAACCCTCAAGTCAAAGTTTCGCAAGCTGTTT
GCGGACTTCGCCACTTATGCCAGGCAAAAGAATCTGAGGGATAAAGAAGAATTCAAGTACCTTGATACTC
AGATTAACCTTTTCAGAAAGTCCTCCCATCTGACAAATACCTTTCGAGTGCACTACATTTACATAGCATA
TAACACCGCCCTGGAGACTACAAAGTTCGTGAATTATTGCAACATCACGAGCTATGATTCTAACCTGCCC
ATTGGCCAGCAGTGTCAGAGGAATGTGCATATTCTCGGAAATTCCCTGCATGAAAATCTGTTGTGTATCA
TGAAGCAGTACTTTAACGCCGATTGCTATTTTAAAACCTATATTGATATTAAACGGCTCAAGAACCCGGA
TCTTAACGTGACCGAGTATGAGTACGCACTGGCCTCCAAGAAAAAAACTATTCAGGCTCTCACATCAGAG
CAAATCACGCGCGCAATCGCTAAATGCAACAAAAACGGCGAGGGTTTGTTTTCCCCAGTTAAACCAGGGC
TCCAGGGCTTGCTTGAAATATCTGCCAGCGATAAATATGTCCAAATTCAGGACAAGAGAATTTACCGCAG
ACAGCATCTCCACAAAGATTACCACCGCCCATTCCCAGTGTTCAGAGTCCAACTTCTTCACAAGAACATT
TTTTGTTTTGGGAATTCCGAGGACTGGTATGAGAACATGGGGTTCAATAGGATCTTGCAGTACTTGCCGG
ACGAGTACATAAGCGATGAAGCCCTGACGCGAGCGATATGGCTGCAGGACACTCATTTCCTGTGTGACGA
CTTCGAGAAGCAGTTTTACACCACCAGACATGAGATCTTCAACGAGAGAATACCAGTCACAAACTATATT
GGAGATCTCGACCTCCCCCTCCAGGACACAGCCACTATAACTGAGGAGACATTCTTTTCTATGTGTCGGC
TTATCCGATTGACCTTGATTAACGCCTGGAAGAAAATTTTCCCCAGTATCGATACCCATACCCATCCCAT
CTTCTTCTTCAAGACCCAGTGTGACACTACAAACGACGCACTGGATTACACTGAGGACCCCACTGAGATT
AAGCAGTTCTGTATTTGCAGAAAGAAGATCGGGCTGCGAATTTCTATTCCTCTGCCCAACGGCACTGCCA
TTGCCGGGGGCGAACCCCTGAAACAGCTCTCTAAAATTCTGAACCACGTTATGTGCTTGGACCAGGAGCT
GAGCCAGATCCTTAATTCATTGACATTTCCTGGAGAATGCTTTGACATTGGAATCTACCATACCGGTCAC
TGCATAAGAATCGGCTACATGTATAAAACCGACATGGATAAAGGCAAGATGCTGCACGGCCGGCTGACCC
CTATCTTTATCGTGCCCGAGGGCTACAGAAATTCCTGCAAGACGTTCATTCAGATGCAGATGGACCTTAA
CAATCTGTTGCATCATGGCACCAAGAAAGCTCCTATCGAAGAACTGATATATTCTATTACAGACAAGGGC
TGTCCCAAAGAGAACCTGTCCTTCATGGATCTGAAGAGTAGACAGCTCTGGAATAAAGTGAACATTGCTA
CAGATACTTTGATCACCAAGTACCTCAACACTCACGGATTTAACAATAATGCGACATCAGCTGATGATTC
TCTCCTGAGCTTCATTCGCCTGATTGGATGGCCCATAATTAAAACTCAACTGATCACCCATTACGAGACC
AGAATCGCCCAGCAATTCTCCCAGGTTACCTTCCTGAAGATCGACTCCAAAAACCTGCAGATTAAAAAGA
CACAGTTTGGCCGAGTGAGCGATTTTAGTTGTCTGAATAGACAGCACAGGGGGAATAGAGACAATGTACT
GGTGTATATACAGTTGAAAGCCGATGGAAACCGACTGATCCTCATCCTGTGGAGCACTTGCTTCGCTACT
AAATGTCAGAGCAACTCCAAACAGGTCCACTGCAGTATCGCACTGGAACAACTGAAGAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101484 representing NP_050224
Red=Cloning sites Green=Tags

MTIVVFATEYDAANVIFSILCRSPSEHLIFPIIVKYKPSNNVSFCLQTQKCKNSKRIDTVFVCHAEKLNL
SHYIQTASPIKAEDVANSLNDKETELLYVDMILSQTGKEKEDVEFKYMAYFHKSLIIKYLTGKFLLPTSP
FWFLSTYGQTEGLLLLTMYYYLFEEQKSTITTTKNYVQCFTENTGSMVFTYSSMSEFINITLKSKFRKLF
ADFATYARQKNLRDKEEFKYLDTQINLFRKSSHLTNTFRVHYIYIAYNTALETTKFVNYCNITSYDSNLP
IGQQCQRNVHILGNSLHENLLCIMKQYFNADCYFKTYIDIKRLKNPDLNVTEYEYALASKKKTIQALTSE
QITRAIAKCNKNGEGLFSPVKPGLQGLLEISASDKYVQIQDKRIYRRQHLHKDYHRPFPVFRVQLLHKNI
FCFGNSEDWYENMGFNRILQYLPDEYISDEALTRAIWLQDTHFLCDDFEKQFYTTRHEIFNERIPVTNYI
GDLDLPLQDTATITEETFFSMCRLIRLTLINAWKKIFPSIDTHTHPIFFFKTQCDTTNDALDYTEDPTEI
KQFCICRKKIGLRISIPLPNGTAIAGGEPLKQLSKILNHVMCLDQELSQILNSLTFPGECFDIGIYHTGH
CIRIGYMYKTDMDKGKMLHGRLTPIFIVPEGYRNSCKTFIQMQMDLNNLLHHGTKKAPIEELIYSITDKG
CPKENLSFMDLKSRQLWNKVNIATDTLITKYLNTHGFNNNATSADDSLLSFIRLIGWPIIKTQLITHYET
RIAQQFSQVTFLKIDSKNLQIKKTQFGRVSDFSCLNRQHRGNRDNVLVYIQLKADGNRLILILWSTCFAT
KCQSNSKQVHCSIALEQLKN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 2580 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050224
RefSeq ORF 2580 bp
Locus ID 1497045
MW 100.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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