DR1 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101440

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for putative DNA-directed RNA polymerase II [Human herpesvirus 6], codon optimized for human cell expression, NP_050176



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CNY 11,500.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101440 represents NCBI reference of NP_050176 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCTGACTGCTCGGGCCGGGCACACCTTGCACCGGCTGCCACTGTCCCACTATTGGTGGCTGCTGC
TTGGGCGCCACAGCTTGCGCCACGTCCACTCTTACTTGCGGTTGCGAAAAGGCCTGCGCCTCCCCCTGCC
TTGGCCAGAACAGGAGTGCCTCCACCTCCATCCAAAGCCTTACAAGTTTTTGCTGCGATATCCATGCCTC
ACACGGCAACCTCATCTGCTGCAGGGGTGGCCCGCTGACTCAAGCCTCTGGTTTGATCCTAAGCCCTACC
ACCCAAGCGCTGACTCTAAGTTGCTTCCCTTGGGACTCATCACGCTGTCAGCCTTCAGCATGAGGGTGAG
CGAGCCGACGCACTGCAGTGGATTTCATGCTGCAGATCCGTCTCTGAGCTGGCTGACCGGCTCTTCACCT
TGGCTGGTGCTCCTCCAGGCCCCTGGCGGTAGTCTGTTTTGTCACGATGTGTTCCAGGGAAGGTTGTACC
TGCTGTCTCACTCCGTCTCACTGTTCCTGAAGACGGGCCTGAGACAGTGCGAGGCGATCTACAGAGCTCC
CCTGTGGCGGGTGAGACCACTGCCATCCCTGTGGACCTGTCGCGACCCTGATACCGCGTTTCTCCCCAAA
CTTCTTGCTAGAACTGCGAGAAGGGGCCTTGCAGCCTTCTACGCTCTCTGGCGCCTGCATCTGGGCTCTC
GGAGCGAGTTGAGTCACCCAGTTCTCGAATGGGAGCGAACGGAACTGGTGCTCACAGACCGCAGGCGCGG
ATGGCCCTGTACTCATCTTCTGTCCGGCTCCGAGAGTCAAAGAGTGTCTAGCTCCGATGCTGGGGACACC
TGGAATGCTGCGACCGAAAAAGCTGCCGGCGGGAAAGAAGAGGCTGAGCGGGGAGGTCGGCAGCAAGCAA
CAGACCGCTTGGCTTCTCCCCATCTTACACGCGGCCGACGGGACTGTGGGAGATCACTGCAGGGTGAGGA
ACCCTCCGCGGCCGAGGATTTCGCCCGATGTAGGCCACTCCTGGATGAATTGTGTGGGGAGGGGGGTTGG
CTCCCTTTTGCCTTCTTGACTGCGTCCCCCCATGTGTGCCTGATCCTGACGGAGGGAGGGCCTGTCCTGG
CCCTGGACCTTAATGATACATCTCTTTGGAGAATCGCCGACGACCTGGAACTTCTCTTGAGGCTGGGCTC
ACTGTTGCTTTTGTCAGGACTTCGGCTGCCCCTGCGCCCACCATCTGGCTCCGGGGAAGCCGCCAGGAAG
CCTGGCTACGAAAAGGAAGAGGGAAGAGGTCGCGCTACCACTGCATCAGCCACAGCGGCAACCTCTCCCC
GGAGACCGACCCGGCCACGGGGCGTGACAGAAAAGGGCCGGGTGACCACAGGGGATGTGCCTTTTAGCGC
CCACCCTGAATCTGAGGAGCAGACCGACGGTCACCATGGAAGGCAGGAATCTGGCCATGGAGACCAGAGA
GGTGGGGATGGCCGCGGCCACCGCGATGATGGAGCTCGCAGGCACGCAAATGATGAGACTGAGCCACAGC
AGAGAGGGGAGCACGAAGACGGTGAACAGACAGACAGTGGGAGGGAGGAGGATGCCCAGGAATCAGAAGT
GGCACGGCGCGACGAGAAAGGCACAGAGCAGGGAGGGAGTGGACGGAGCTGTGGAAGGGCAACCCAGACA
TACGGTGGACGAGGCGAGCACGGGGCTTGGTCCAGTATCCCTTTGAGCGTTCCGAGACCTGACCCTCGGG
TGTGGGTTCCACCACCCCACCTGCTGTTCCCTAGTCCTCTCCCTAGCATTACACCAGTGGAAGATGAACC
CAGCGCACGACCCCGCTGTCCACCAGGACCGGCTGAAGAACCATCCAAGTGCAGTCCCTGCCCACCTTGC
CCCTCACCTGATGCCCCACAATCAGCTGTGCCGCGCCTTAGCGCATTGTCTGTGCCTTCCCCCAGCACAG
CACGGGTTCGCTTCAGTCTCTCTTCTCTGTCTAGCTCATCATCAAGTTCAAGCAGCAGCAGCCCCTCTTA
CTCTCCCAGTCCACTTAGCCCCCCTTCCCCCGTGTCACCTTCTTCTCCGAGATCCCCCTTTATCTCCCCT
ATAAGATCACCCGGCCTGCGCGCCAAACCATGGGTTAGTTCCGGTCATCCTGTGGCTTTCCCTCCCGCTC
CCAGCAGTGCTCCACCCTTCTCTAAGCGGGTCCCCAGCGTGCCAAGCTCTGCCTCTCCCAGCGCTCCGTG
CATCGGACGGTCCAGACCCCCTAGCGCCCAGACCGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101440 representing NP_050176
Red=Cloning sites Green=Tags

MPLTARAGHTLHRLPLSHYWWLLLGRHSLRHVHSYLRLRKGLRLPLPWPEQECLHLHPKPYKFLLRYPCL
TRQPHLLQGWPADSSLWFDPKPYHPSADSKLLPLGLITLSAFSMRVSEPTHCSGFHAADPSLSWLTGSSP
WLVLLQAPGGSLFCHDVFQGRLYLLSHSVSLFLKTGLRQCEAIYRAPLWRVRPLPSLWTCRDPDTAFLPK
LLARTARRGLAAFYALWRLHLGSRSELSHPVLEWERTELVLTDRRRGWPCTHLLSGSESQRVSSSDAGDT
WNAATEKAAGGKEEAERGGRQQATDRLASPHLTRGRRDCGRSLQGEEPSAAEDFARCRPLLDELCGEGGW
LPFAFLTASPHVCLILTEGGPVLALDLNDTSLWRIADDLELLLRLGSLLLLSGLRLPLRPPSGSGEAARK
PGYEKEEGRGRATTASATAATSPRRPTRPRGVTEKGRVTTGDVPFSAHPESEEQTDGHHGRQESGHGDQR
GGDGRGHRDDGARRHANDETEPQQRGEHEDGEQTDSGREEDAQESEVARRDEKGTEQGGSGRSCGRATQT
YGGRGEHGAWSSIPLSVPRPDPRVWVPPPHLLFPSPLPSITPVEDEPSARPRCPPGPAEEPSKCSPCPPC
PSPDAPQSAVPRLSALSVPSPSTARVRFSLSSLSSSSSSSSSSSPSYSPSPLSPPSPVSPSSPRSPFISP
IRSPGLRAKPWVSSGHPVAFPPAPSSAPPFSKRVPSVPSSASPSAPCIGRSRPPSAQTA

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 2277 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050176
RefSeq ORF 2277 bp
Locus ID 1497018
MW 82.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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