U30 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101471

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for capsid assembly protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050211



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CNY 16,340.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101471 represents NCBI reference of NP_050211 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCACGACAACAGGCAGCTGTTGCCCCAGGAGACCAAGCTGGTCACACCCAACAAAGATACTCTGT
CTAGTCGGTACCTGCACGTTCTGATAATTGATAACACCCTGAGCACTATAGAATTCGTGTATATGGTGGT
GAAAGCCGTGCTGACGCAGGCTGACACCCTTAAAGCCTTCGAGCAGAGGAAGAACAGGCCAACAAATAGG
ATCCTGGGGCTCAGCACCAGCGTTTCAAAGAGATACATCAATATTCCGAAATTTAGCAGCTCAGGCGCAA
ATAACTCACAGGGCGTTAAAACGCTCGCCGCCATACGCAAGTTTTCCTTGCCCGACAAGAATTGTTTTCG
AAACTTCCTGAGCTTCTCAACCACTCTTTTTAAGGTTCCGGCCTCCATCGACATTTATTTTCTTTTCTTC
ACAGCCAGTACCGTTTCTACTATGGCCGCACGGGCTCTGGACCTTGAGTTCATCCTCTCTAGCTTGAAAA
ATTCTACAAGTCCCGAGTCCCTGCTGGCAGCCACAGCTAAAATAGAGATTCTGTCTCTGGCTGCCGATTC
AGTAACCCATAATCGAGTGATCGCATTTATTAACAGACTCCCACCTAAGAAATATCACTTTGATCTCATC
CGAGAATATACAGTTTTCTACTTCCTGAATAGTACAACCCTCACCTCCGAGAATAAGCTGCTCAGCGCCG
AGCTCCTCCATGAGGAACTCTCCCAGATCCGGTCTTCTTCCTCACCTGGGACCGAGCTCGAGAACCTGAA
TAACGCTGAGGTGTTGTATGTTTTCGATCGGATACTGAATAGTATAAAAATGCTGAAGAACGAACTCTCT
AGCCCTATAGGTAAGCTGCGGGTGCAGCCCGCGGCAAACGATCCCGGGACTGATAAGACTATCAAGTATT
CCAAAATCCAAAGTATTATCCAAAAAGTGCATAGTTTTACAAGGGAGAACAGCTTGCTCGCGAACTGCAG
GGCCGTGGTGGAACTGATTGATGATCTGTACCGGAAACTTTATAGCTGGTTTCTGCACATTCTGACATTT
GAAGATATTCAATTTCCGGGGGATACCTTTCTCGACCGCCTGCTGAAGATGGACTACTGTTTCACATATT
ATCCTAGCTCTAATAGGCACCTGATAGACCTCTTTGAAAAAACTTTGGACAATCAGACTTCCACCGACAT
CGATAAGTTCTTCGACACTAGCGGAAATAGCCCGGAGCTGCTCTACCAGAAAACCTTTAGCCTGAAGATT
TTTAGCAAAAATCTCACGGCACAGGACAATGGCCTGTACATCTATCCGCTGTTGAAAACCGATCTGAGCA
TTCTGGATTTTCTCGGGACCGAAAACATCCTTTTCCATAGAGGACTGATATATCACATCCTGCATCAGAA
AACGATCCCGCAAGAACGGGAGAACGATCTGAATAAGATCAATCAATTCTTCGCAACGGTAATCCAACAA
GTCATTGAGACTCGCAGTTCTTGTCTGCCTGCAAGCCTGTCCAGGCTGCTGGACACTATCTTTCACTTCA
ATCGGATTGGACTCAACATCGAAACATGCAGGATTTACGTGGAGATACTGTCAAACCATATGGCCACCCC
CGACACACAGCCAATCATTAACACCTTCACTATAAATCTTATCCACATCGTATTTACCGCGCATGTGTTC
TTCATTTGTATGGAGAACTTCTCTCCCACCTTCCTGTTTTACAACAGGAAGAAACTGATTCTGGAACAGC
AGCGCGCCATCTTGATCATTGAACGAAACGAGTATTCAACTCTCTGGAAGCAGATTTCTGATCACATCGA
CTGCTTGTTCAATATCTCTCTCAGCGAAAGTTTCTTCAAGGAATACACTAAAGGAGGCAACGAAGAACAC
AAACAGTTCCTGTATAAGAATCTGTTTGAGAAATGGGGTGATGTGTTTTTCCCTTTTACGTATAGTGTGA
CCACCTCAAAAAATTCTACAGCACATCATATCACGACCCTGGAGCTGCGGGCTATATGCAAGGAGGTTTA
TCAGTCTGACTCACCTGAAGCGTATGAATCTCTTTTGCCATACTCTACCCATCCCGCATTCAAGACTCTG
TTTATTAAGATTTATGTCATACCCATGGTTACCTATATTACTAATCTGACATTCGACAAACTCCAATCTG
ATTATAGGCTCATTACTCTGATCCATGCTTGTAAGCTGCTGTTGCCCTCTCAGCACCTGCTCCTTCACTA
TATGGTGTGGTTGTACGCATTTAGCATTAACGTCGACCACATAGATCTCGGAACGTTCACAGTCATCAAA
TCTGTGATCTTTAAGATCGCGGACCATATAAACGTGATGACTCACACAATTTATTCTCCCGAGACTAATC
TCCTCGTCTCTATTCTTCTGAATGCCTACACTGACTACCTCCAGAAATACGTAAATCCGTGGATCAAACA
GACGATCACAGCTAACTTCTCACTCCTTCAGACTTACATTACCTTCACCAAGCAGTGCGCCTCTATCTTG
GCAACCAAGTGCAATATCAATCTGGACAATTTGTTTATCTCAATGACAATCGGAACTGACAAGATTGTTA
CAACATCTTTCTGCAGCTTCATAGCAACATGTCGAAACCTGGTGAGACAGCACGAGGAATTCAAGAAGAG
TCTTAAGACCATTGAAACCAGTAAGACCACTCTCACCAACATGCTGCTTAATATTATAACAAGCGTGTCA
TCATCCAAGGAGCTGTTGACAAACGAGGCGCTTCAAAAGTTCATTGACACCGTCCAGAGGATCTCACAGC
ACGTTAACGAGACTTACCAGCTGATTAGTGTGAACCTGGAGAAATGTAAGATTTCAAACGATATCTTGAT
TGAAAGTCTGAAGAAAACTATTTCAATTGTCGACGTGCTGTCCTCCGATGCCATCCTTAATACCAGTCTG
ACTTCACGCTGCTTGGAAGCCGCTACCCTTGCTGTGTCAAACAACTCATTCACGATTCTCGAGATTAAGA
AGGACGCAGTCGCCGTCTTCAAACCATTTATCACCCAGCTTTTCGAAAGCATGAAACCAACAACAAGCTT
GTACAAAAAGCTGATGGCCACCCAGAAATTGACAACCGACCGCATCCCTTTCTTGGACATTTTCGACGAT
AGGTACAACCTGGTCAGGCACGTGGAGAGGCAGCTGAACTGGTATGCTGCTTATGCTGAGGCAGCCCAAC
AGGATCTGATTGCCCCACTTACTTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101471 representing NP_050211
Red=Cloning sites Green=Tags

MTHDNRQLLPQETKLVTPNKDTLSSRYLHVLIIDNTLSTIEFVYMVVKAVLTQADTLKAFEQRKNRPTNR
ILGLSTSVSKRYINIPKFSSSGANNSQGVKTLAAIRKFSLPDKNCFRNFLSFSTTLFKVPASIDIYFLFF
TASTVSTMAARALDLEFILSSLKNSTSPESLLAATAKIEILSLAADSVTHNRVIAFINRLPPKKYHFDLI
REYTVFYFLNSTTLTSENKLLSAELLHEELSQIRSSSSPGTELENLNNAEVLYVFDRILNSIKMLKNELS
SPIGKLRVQPAANDPGTDKTIKYSKIQSIIQKVHSFTRENSLLANCRAVVELIDDLYRKLYSWFLHILTF
EDIQFPGDTFLDRLLKMDYCFTYYPSSNRHLIDLFEKTLDNQTSTDIDKFFDTSGNSPELLYQKTFSLKI
FSKNLTAQDNGLYIYPLLKTDLSILDFLGTENILFHRGLIYHILHQKTIPQERENDLNKINQFFATVIQQ
VIETRSSCLPASLSRLLDTIFHFNRIGLNIETCRIYVEILSNHMATPDTQPIINTFTINLIHIVFTAHVF
FICMENFSPTFLFYNRKKLILEQQRAILIIERNEYSTLWKQISDHIDCLFNISLSESFFKEYTKGGNEEH
KQFLYKNLFEKWGDVFFPFTYSVTTSKNSTAHHITTLELRAICKEVYQSDSPEAYESLLPYSTHPAFKTL
FIKIYVIPMVTYITNLTFDKLQSDYRLITLIHACKLLLPSQHLLLHYMVWLYAFSINVDHIDLGTFTVIK
SVIFKIADHINVMTHTIYSPETNLLVSILLNAYTDYLQKYVNPWIKQTITANFSLLQTYITFTKQCASIL
ATKCNINLDNLFISMTIGTDKIVTTSFCSFIATCRNLVRQHEEFKKSLKTIETSKTTLTNMLLNIITSVS
SSKELLTNEALQKFIDTVQRISQHVNETYQLISVNLEKCKISNDILIESLKKTISIVDVLSSDAILNTSL
TSRCLEAATLAVSNNSFTILEIKKDAVAVFKPFITQLFESMKPTTSLYKKLMATQKLTTDRIPFLDIFDD
RYNLVRHVERQLNWYAAYAEAAQQDLIAPLTF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 3246 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050211
RefSeq ORF 3246 bp
Locus ID 1497032
MW 124.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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