U76 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101515

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for putative viron protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050255



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CNY 7,890.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101515 represents NCBI reference of NP_050255 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATAGGGCAAGCGCCAACTCTCCACTGAACTCCGTCAGCGGGAGTATGATGTGGCGGAATCAGTCTA
GCGGTCGAAGACCGAGTAAGAGGCTCAGTGATAATGAGGCTACATTGAGCACCATCAATAGCATCCTGGG
GGCCGAGGACATGCTTAGCAAGAACCTTCTTAGTTACCTTCCTCCTAATAACGAGGAAATTGACATGATC
TATCCGAGTGAACAGATTATGACCTTTATCGAGATGCTTCACGGTCACAAGAATTTTTTTAAGGGTCAGA
CCATCCATAACGCATTGAGAGATTCTGCGGTTCTGAAAAAACAGATCGCATATGGAGTCGCCCAGGCACT
GCTGAATTCAGTCTCCATCCAGCAGATCCATGATGAGTGGAAGAGACATGTGCGGTCCTTTCCATTCCAC
AATAAGAAGTTGAGTTTTCAAGATTATTTTTCAGTCTGGGCTCACGCCATCAAACAAGTGATCCTTGGCG
ACATCAGTAACATCATCAACTTTATACTGCAGTCCATCGACAACAGCCATTACAATCGATACGTAGACTG
GATCTGCACAGTGGGGATCGTACCATTCATGCGAACCACCCCCACCGCTCCAAATCTGTACAATCTGCTG
CAGCAGGTGAGTAGTAAACTGATTCACGACATAGTTCGACACAAGCAGAATATCGTGACTCCAATTCTCC
TGGGTCTGAGCAGTGTGATCATTCCTGACTTCCATAATATAAAAATCTTCCGGGACCGGAACAGCGAACA
GATCTCCTGCTTCAAAAATAAAAAGGCAATCGCCTTTTTTACCTACTCTACCCCCTACGTGATCCGCAAT
CGACTCATGCTGACTACCCCCCTGGCTCATCTGTCTCCAGAACTGAAGAAACATAACTCACTGCGAAGGC
ACCAGAAAATGTGCCAGCTGCTGAACACCTTCCCAATTAAGGTGCTGACTGCCGCCAAGACCGACGTCAC
CAACAAAAAGATCATGGACATGATCGAGAAGGAGGAAAAGAACAGTGACGCCAAGAAGTCTCTGATCAAA
TTCCTGCTGAACCTTTCCGACTCCAAATCCAAAATCGGTATCCGCGACTCTGTCGAGGGCTTCATTCAGG
AGATTACTCCCAGCATTATCGACCAGAATAAGCTTATGCTTAATAGGGGGCAATTCCGAAAAAGATCCGC
GATCGACACGGGGGAGCGGGACGTGAGGGACCTGTTCAAGAAACAGATAATCAAATGCATGGAAGAACAG
ATCCAAACTCAGATGGATGAGATCGAGACACTCAAGACCACCAACCAGATGTTTGAGAGGAAGATTAAAG
ACCTGCACAGCCTGTTGGAGACTAATAATGATTGTGATAGATATAATCCGGATCTGGATCATGACCTGGA
AAACTTGAGTCTCTCTCGCGCTCTTAATATCGTACAGAGACTGCCCTTCACGTCAGTGTCTATCGATGAC
ACCCGGTCCGTGGCAAATAGCTTTTTCAGTCAGTATATTCCCGACACCCAATATGCTGACAAGCGGATCG
ATCAGCTCTGGGAAATGGAGTATATGAGGACATTTAGATTGCGCAAGAATGTGAATAATCAAGGGCAGGA
GGAATCTATAACATACTCTAATTATAGTATCGAGCTGCTGATCGTGCCCTTTTTGAGGCGCCTTTTGAAT
ATATATAACCTGGAAAGCATACCCGAAGAGTTTCTCTTCCTTAGTCTTGGAGAGATCTTGTTGGCAATCT
ACGAGAGTTCAAAGATTAAGCACTACCTTCGCTTGGTGTATGTGAGAGAGCTCAATCAAATCTCCGAGGT
CTATAACCTGACTCAGACCCACCCCGAGAATAACGAGCCAATCTTCGACAGTAACATTTTTAGTCCCAAC
CCCGAAAACGAAATTCTCGAGAAAATCAAGAGGATTCGGAACCTGAGGAGGATCCAGCACCTGACAAGGC
CGAATTATCCCAAGGGCGATCAAGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101515 representing NP_050255
Red=Cloning sites Green=Tags

MHRASANSPLNSVSGSMMWRNQSSGRRPSKRLSDNEATLSTINSILGAEDMLSKNLLSYLPPNNEEIDMI
YPSEQIMTFIEMLHGHKNFFKGQTIHNALRDSAVLKKQIAYGVAQALLNSVSIQQIHDEWKRHVRSFPFH
NKKLSFQDYFSVWAHAIKQVILGDISNIINFILQSIDNSHYNRYVDWICTVGIVPFMRTTPTAPNLYNLL
QQVSSKLIHDIVRHKQNIVTPILLGLSSVIIPDFHNIKIFRDRNSEQISCFKNKKAIAFFTYSTPYVIRN
RLMLTTPLAHLSPELKKHNSLRRHQKMCQLLNTFPIKVLTAAKTDVTNKKIMDMIEKEEKNSDAKKSLIK
FLLNLSDSKSKIGIRDSVEGFIQEITPSIIDQNKLMLNRGQFRKRSAIDTGERDVRDLFKKQIIKCMEEQ
IQTQMDEIETLKTTNQMFERKIKDLHSLLETNNDCDRYNPDLDHDLENLSLSRALNIVQRLPFTSVSIDD
TRSVANSFFSQYIPDTQYADKRIDQLWEMEYMRTFRLRKNVNNQGQEESITYSNYSIELLIVPFLRRLLN
IYNLESIPEEFLFLSLGEILLAIYESSKIKHYLRLVYVRELNQISEVYNLTQTHPENNEPIFDSNIFSPN
PENEILEKIKRIRNLRRIQHLTRPNYPKGDQD

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 1986 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050255
RefSeq ORF 1986 bp
Locus ID 1497076
MW 77.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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