U95 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101530

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for immediate-early protein IE2 [Human herpesvirus 6], codon optimized for human cell expression, NP_050270



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CNY 18,340.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101530 represents NCBI reference of NP_050270 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCAAACCTGGAAGACCTCCTGTGGCAGCAGATCCTGAGTATGGATCCCGCGGAACTGCTGAGCG
ACAATGCCATATCCAGCACGTGTGACGAAAATATCGCCGCGGGGCACCACTTTACCCAATCACCACACGT
TGAAATGAGCGTGCAGTCTACAACGTCAGCCGGACACACAGGCGTAATGACGACACAGTCACAATTTAGT
AATGGAGTACGCGACCAGAATCGCGAGTCCTTGAGCACACTGACCGGGTTGAGCTTGGAATCCATTAATA
ATCAGATCAATGTCCAGCCTACTCAGATGACCTTCCAGCCGATTTCCCCGCCTATGCAGGGCCAGAACTA
TGTGTATTCAAATAATATGATCAACCCCATTAAGCCCCGGTCTATCATTAAGTCCCATGGCCATTCCATG
GGGGAAATGTCCTTTGCAGACCACTCTCTGTACGTTAACGCACAACCCCCCGTGCAACAACCACAGCTCA
AATCACTGGTGGGCATGCATCCTTGCATGACTGCTACATCCCAGGGCAAATATCAAACGAATAAAACAGT
GGGACCCCCAAGCATTTCCGCCAGCCAGATTACCACTGGCAATGCTGGGATTAGACCAGGGGAGTATCAG
AGTGTCCATAACCAGAGCAGCGTCAACGGCAGTAAGAGTTACGAAATAACTACCGCTAGTGGCGACGAAT
GGATCCTGACCACACCCGGCGGCCAGAGCTGGACACTTAAGAGAAATCCTCCTAATCCCCCAAATAATAG
AACTAACTCCGTCGTGAATAAGGCTCAGCAGGTGTCCCACGCACAACCTTACGTTAGCGGATCTAGTGAC
GGCTTCTACCAGGGAGCCGCGCTGCAGTCATGCGCCTATGTGAATACCCCAGGATTCACGCCCGTGTGCG
AGACGCAGAACATGAACAACAGTCAGGCCACCCAGCTGAGCGCATCAATGAACTGCATAAACGCCCTGCA
GACAACCATGGACGCGATCGTTACGAGCACCAGCAAACCCGTGGGCGCCGTCAGCAACAATCGCGGAGCC
AATTTTGGAATGGGCGGCATGGAGAATTACATGGATAACAACTCCCCTTGGAACCAGTATTGTAAGGTAC
AAGACATAGTTTCACAAAATTGTAGCCAGGGTAAGGTGGTGAGCAGTACCCCTGGGATTGCGCCTAACTT
GATGAAAGGGAATGGATTGAACGTGTACGGGCACGTTGGCTGCGTGGACGCCGCCATTTCCGACAAGCAG
GGGGGAACAGCTAACGTGGCCAGCTCTCTGCTCAACCCGGAGCATCAGGACTGGATGAGAGTCACGGGAA
CCTCTACCAACCTCCTGAACAACATCAATGCCGAGACTAAAATGGAAAACTACGGCTTCCCAGAAAACGG
AAACGTCCATGGCGCCGTAAATACCGCCCTCCCACTGACTCTCTCTTCAGGTCAGCCATATACCTCCGTA
CCCCAGCACGGGGCTTGTGAAGGCAACGGAACTATTCCAGTGGTGCAGATCTGCTCTCCTAATACGGCTT
TTAAGGCTCACTACTCCTTGCTCGGTACGGTCGATGAGAACAACCCTCTCTCTGTGCGGGAATCAATTCA
GGACACGTCTTTTAGCAACGGCTGTGCTCCCCAGTTGAGCTCTCCTGGGGGGAACCCAACAATCATAGCC
CACAGTATGATAGGCAATAACGGCACGCCCAACAAGGACGTCTGTAAGCCTACTCCCTCACTCAGGGCAA
TTAAAAAACTGAATTTTGATTACGATGATCGAGGTGAAAACATTGGCTTCCCAAGCAAGCTGGCCGCCCT
TTTGTCCATGGGAGAAAATATGTCTAAAATGGACAACCCGTGTTACGGAACCTCCCTCGCTCAGTTTGAA
GAATCACATCAACAGAACGCAAGTGAGGGTAAAATATCTATCGCTGATCTGGAATTCTCTGAAGAAGACG
ATGTCCTGAGTTCCGCCGCGTCCGTGTCCTGCAACGACAACTGCGTCATGAAAATTGGTGCTAGTCAACA
AGGGACGACCGTGGCCGACCTCCAACAGGGCTTTAAACAACAGATGAATGGGGAATTCAGCATGTTCGCT
GTGGATGGAAACATTAAAACTCAAGAGATGAGTAACGACTGCGCTTCAAACGTTACTGACAACGCCTGTG
CAATAAGGCAGAACAAACGGATGCACTGCGAGATTGGGATCTCTGAAGATGGCCGCGTCAGGGAGGAAGA
GAAGTGCTCCGATGTAGCTATCCACGTTCCAAGAAAAAGCGCAAGGATTCACAACATGAAGAGCGAGGGC
GTCACTTGCGGAATGTGCGTCACGGCTGCCGACTCTACAAGGCAGGATGCCAGCGGTGGGAGCAGCTCAG
GAACCAAAAAGGGCGAGAAGCTGCAGGGCCTGTGGAAAGGATACCAAGACGACGATGACTCCGAGTTGAC
CGAACTTTCTGACACTGACAGCGATAATGATGTGCAGAATTGTCATGGGGTACGCAAGACTGGAAGCAAA
ACGTACTCCAGTGTCTTCTTCAATCCTGATTACCGGCAGGCTAAGAGGCTCCTCGCAGACATCCCCTACA
GAAGGTGGATCCCCGATACTTTTAACATGGAGGAACATGAGGGACCGTTTCTGCCGATCGTAACGAGGCC
CCCCACCGTGTTCATGGGTGGACGGAGAAGACGGACCTATCTCAGAAGAAGTGTGACGAGTATCGGCCCA
CTGAGTAAGTTGACATACTTCAAAGAACTTCTTCAGTCCTATGTGCTTAGAAACAGTAATTGCTACCTGA
GCATCGGCTGGCCCGCTAAGCACCGGGTGTACATCATGTCTGAGGAAAAACTTGGTTATAATCACATCCC
TACTCTCAGAGAAATGTTTCCTCTTCCACCGGGATGGATGATAGTTCTCGGAATTGTGGGCAGTGAAACT
CCTGCCGCGCTGTATAAACATATGGTGGTGTTGCTGTGCGAGAACAAATGGGTCCTGCTGCACAACTACC
GAGATAGCAAGCACGAGCTGTACTTTGCCGCCTCTGATCTGAAGCAGTTCATGGAAGAGGGACTTAGCAG
ATGCGACTGCATTTACTACGAAAAGTCAGTCCCATACGGTGTGGCCATGGAAGATTCCGTCAGGGAGTTC
CTGAGAAACTCTAAGACGTTTCAGTCTCTGATGGAGTACCGCAAAAACATGCACGGAAGCACTTGGACAT
TCAACGGCATGCCAGGCCGGCTGGGGGACCGCGTGATTCATATCTGCAACCCTGAACTGGTTAACTCCAT
CCCGGCGGATGAGGCCATACGGTACGAGGGAAAGCCGCTGTACTTCTTTGCATTTGTTACCACGTTTAAG
TCACATCCCGGTTCCAAGGCGAACGTGCTTATCGCGGCCGACAAAAACCTGGGTATCTATGGCTATCATA
AAGGGCGCCCACGAATTAGATATCTCTGTAAGAACGTACAGGCTTTCTTTAGGGCAGGAGTGCGCAAGAT
GTATCTGGATTATGAGATCCCGAGCAAGACCCTCCTGGCGGTGTCCGAAGACGACTACCTTTGTATCCTG
CAAAAGGCCCCATGTCTGCTTCTTAAGCCAGCGGTGTTCCGCAAGACCTTTAGTCAAGAAGGCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101530 representing NP_050270
Red=Cloning sites Green=Tags

MSSNLEDLLWQQILSMDPAELLSDNAISSTCDENIAAGHHFTQSPHVEMSVQSTTSAGHTGVMTTQSQFS
NGVRDQNRESLSTLTGLSLESINNQINVQPTQMTFQPISPPMQGQNYVYSNNMINPIKPRSIIKSHGHSM
GEMSFADHSLYVNAQPPVQQPQLKSLVGMHPCMTATSQGKYQTNKTVGPPSISASQITTGNAGIRPGEYQ
SVHNQSSVNGSKSYEITTASGDEWILTTPGGQSWTLKRNPPNPPNNRTNSVVNKAQQVSHAQPYVSGSSD
GFYQGAALQSCAYVNTPGFTPVCETQNMNNSQATQLSASMNCINALQTTMDAIVTSTSKPVGAVSNNRGA
NFGMGGMENYMDNNSPWNQYCKVQDIVSQNCSQGKVVSSTPGIAPNLMKGNGLNVYGHVGCVDAAISDKQ
GGTANVASSLLNPEHQDWMRVTGTSTNLLNNINAETKMENYGFPENGNVHGAVNTALPLTLSSGQPYTSV
PQHGACEGNGTIPVVQICSPNTAFKAHYSLLGTVDENNPLSVRESIQDTSFSNGCAPQLSSPGGNPTIIA
HSMIGNNGTPNKDVCKPTPSLRAIKKLNFDYDDRGENIGFPSKLAALLSMGENMSKMDNPCYGTSLAQFE
ESHQQNASEGKISIADLEFSEEDDVLSSAASVSCNDNCVMKIGASQQGTTVADLQQGFKQQMNGEFSMFA
VDGNIKTQEMSNDCASNVTDNACAIRQNKRMHCEIGISEDGRVREEEKCSDVAIHVPRKSARIHNMKSEG
VTCGMCVTAADSTRQDASGGSSSGTKKGEKLQGLWKGYQDDDDSELTELSDTDSDNDVQNCHGVRKTGSK
TYSSVFFNPDYRQAKRLLADIPYRRWIPDTFNMEEHEGPFLPIVTRPPTVFMGGRRRRTYLRRSVTSIGP
LSKLTYFKELLQSYVLRNSNCYLSIGWPAKHRVYIMSEEKLGYNHIPTLREMFPLPPGWMIVLGIVGSET
PAALYKHMVVLLCENKWVLLHNYRDSKHELYFAASDLKQFMEEGLSRCDCIYYEKSVPYGVAMEDSVREF
LRNSKTFQSLMEYRKNMHGSTWTFNGMPGRLGDRVIHICNPELVNSIPADEAIRYEGKPLYFFAFVTTFK
SHPGSKANVLIAADKNLGIYGYHKGRPRIRYLCKNVQAFFRAGVRKMYLDYEIPSKTLLAVSEDDYLCIL
QKAPCLLLKPAVFRKTFSQEGK

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 3636 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050270
RefSeq ORF 3636 bp
Locus ID 1497091
MW 133.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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