U41 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101482

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major DNA binding protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050222



  View other clones from "Virus" (98)

Need custom modification / cloning service?
Get a free quote

CNY 17,100.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101482 represents NCBI reference of NP_050222 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACGAAAATGAAACTGTCGTTTCCGCTCCCGTCTCCACTGCCGCCTGGATCTACGTCTTTCCAA
AGGAAAAGGAACTGCTCGACGTCCTCTCCGTGCTGAGCCTTATGGAACGGAACAGCCCAGTCGTAATATC
ACCCCTGTTGATGAATCTGACTGTAGAGAATGACTTCAGCACCACCGTAAAGACACCTATCACCAATTTC
GGGGGAACCATCCTCACCAAGATTACCTCCTTCATGCCTGTGTGCTTCTTCTTTCACGGGACAGAGCAAT
TGGTTGGGATGGCCGAGGATCATGGCGATCTTATCCGGTTGTGTGAGCAGACCCGACAGAAATTCCATTT
GCAGTCTTTTGAGGTTCCCACTGCTAGAAAGGTTATCGACATCAAAGCGTTGTGCTCCGCCGTAGGCAAA
GACGCCGATTCTGTGATCTGTCACGTAGCTTGCGGCAACGGATTCAAGGAGCTGCTGTTTGCCGGCTTGC
TCATTCCTTGCGTGGAAGAGCAAATTCAGGTTCAAGTGGGCGAGTATTCTTGCGTAAAGATCCCGTTGTA
CAGTGCTACACTGTTTGAAACCGAGGAAACCATCTCCCTGTCCAGTTGTACTGAATTCATACAGGAACGG
GGGTTTTTTCTTCCCGCCTTGTCAGAGACTCTGTTCTATTATGTGTTTACCTCTTGGGGTACAACCCTGC
GATTCAGTAATACCAAAGAGTTGATCGACGCAGGGCTGAAGCAGTTTACCCAGGATGGCGAGCAGACAGT
CAAACTGGCCCCTCACAAGACCTACCTCGGAATCAGCGGACAGAAAATTAGCGCAGTTGAGAAGGACTTT
TTGATGTTGGTCGACAGTGTCGTGACCGAGCTTTCTTTCAGTCACGTTGCGGAGTACCTGGACAGCGTCT
ACGACCCATCCCAAATTATGAATTTTAACGATTGGCCTATTATTCGCAACAGCGAGACGCATGCAGAAAG
GATGGCCCAGCTGACCAACCTGAAGCTGCATCTGAGCTCCCATCTTGCTGTACTGATTTTCGCACCCAAT
AGCATATTGTATTGCTCAAAATTGGCTTTCATCCCCAACGTCAAACAGGCCTTCAATTCAGTGATGACTC
AAGAGCTCCTGCTGCGCTCTCTCTCTTTCTGTAACGCTCTGTCTAGCCTGACCGAAGACGTCTACAATGA
CAATCGGAAGATTATCAAGTGCGATAGCACCAGCGGTAAAGATGATAAATTTTCTGCTAATCACTTGGCC
TATGCCTGTGCTACCTGCCCCCAATTGTTGAGTTATGTTGTCTGGAACCTCAACCGGATGTCCGTGTACA
ACGCGGGAAATGCGTACACGGAGATTTATAACCATCTGGTGAACTGCTCTGCAAATCTGTGTGAGTTCTG
TGATGGTAAGTGCTGCCAGAGCTGCATAGGCACTGCCATGGTTCGGGTGGGGACGAGACTGCCCGCCATT
CCCAAGAATGTGAAGAAAGAGCCCTTGGTGATGTCCATGTTTAGCCGGTACTACGCTGAGGTGGATATCC
TCGGATCCTTTGGCCGGAAGCCGGTGTCCGAACTGAAAGAAATCGGCAAAGACCAGCAGAATACACTCAG
TCTCGATCGCGGTAAGTTTGTCTCCCAGATATTCGATTACTGTAAGAAGAATTCTCTCATCGATCCAGTC
ACCGGAGAGGATACGTTTAATGTGCGGAGTAAGAAAGACTTTGTCTCCATTATTCATGGGCTGACTCAGT
GTATTGAGGAGTGTGTGAGTAGGTGCATCGTAGAGATGCGCAGGACCCAGACCCCCAGAGAACAGATCGA
GAACTGCTTGCAGAGCTTTAACGTCGACACCACCCCATACGCTACAGCTTTCTCACCCTTTCTGACATTC
GCCTACTACAAAGTGATACTGACTGTGCTGCAGAATCTGGCCCTGATCGTGGCAAGTGGGCACGTCGTGG
ATCGACCATGCACCGGGAACAGTATTAGCAAGTGGCTGGTGCAGCAATACCAGTCCTTGTACGGCACCTT
TCACTCATCCTATCTCAAGAAGGGCTTCCTTAATACCCGGACCGTGAAGGTTGCTTCAAACGTCGATATG
GAGCAAATCCTGGACTGTGACCTGTATAAATCTGGGAAGTATGTCAAGACCACCATCCAAGCGAAACTCT
GTAGGCTCTCCATGCAGTGCCTCAGGGATTTCCGGATTAAGAATAGACCTTTTAACAAATCCTCCAAGAC
GGCGCACAATAACCCTTATTTTAAAAAGAATGTAAAACATAAAAAGAACCCTCTGTCAGGTTGCATTTCC
TTTTTGCTGTTCAAATACCACGACAAGTTGTTCCCTAATGTTAAGATCTCATGTCTCGAGCTCTGGCAGC
GCTTCCTGCTGAATAACGTTCCCAAGACACTGGACATAGGCAATCCTGAGGAAGTGAAAACATTCATTAA
GTTTGCCTTTTCCATCACTAATACTTATGATGAGATTGACATCATTGATATACAGCCGGAATGCCTGTCA
ACATTTATCGATTGCTATTTTCACAATAAATTTCTCAGCGCCCTGGGATTCCATGACTATTTGACTTCCT
TGCACGGACTCACCTCTAAACTGGTTACCCAGAACCCCGTATTGTTCCCGGTGGTACTTGACAAGCAGCC
CAAATTTTCAAGTATACAAGAGTACCTGGTTTATGTGAAGAAGTTGGTTCTGGACGGTGTCCCTAATCCC
GTGATTGCTAGCTTGTCTAAGGAGCCAAATTTCGGCACTATTTTTACCAGTAGGAGTTTGGTTACCTTTG
GTCTGACATTGGAGAAGTTTGTGAGTCTCGCAAACCGCGAGTATTTTCAGTTTGGGCAACTCGGCTGGAT
TGGAGGATCCGGGGTCGACCGGAACCTGAATCCAACATCATCTGCCCTGCAAGATTTTAGATTTATGAGA
CAGAAGACTATTATCGCCACAAAATTCTCTGAGGTGATCGTGAAGAAGGTACGCAGGGAGACTATAATGT
TCGATACCGAGGTAGTGAAAGGCAAAGTGCTGTCAATTGTGGAGAACCTCACAAACGATATAGATCCCGA
GTTGCTGATCATTGCCGAGGTTATGCGGGACCGGGAGGATAAGCCCACGATGGATGACATGCTGTTCTTT
GTAGACGGGAGAGAGGCCCTCGCCGCCTCAATCATGCTGAAACTGAACCACCTGGTGGATATGAACGTGC
GGGATTTTAGCATCGCCAATCTCCAGTCCGTATTCGAAGCAGTGTCCTCTAACGATGCTCCTGTCTACGA
TTTTTCTGAAATCCTGGCTGAAGAGGATGACCAGGCCAGCGGAGTTCTTAAGTGTGACGAAACGGAGACC
GAAACTGACGAACCTATGACTAAAAAGAACAGACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101482 representing NP_050222
Red=Cloning sites Green=Tags

MADENETVVSAPVSTAAWIYVFPKEKELLDVLSVLSLMERNSPVVISPLLMNLTVENDFSTTVKTPITNF
GGTILTKITSFMPVCFFFHGTEQLVGMAEDHGDLIRLCEQTRQKFHLQSFEVPTARKVIDIKALCSAVGK
DADSVICHVACGNGFKELLFAGLLIPCVEEQIQVQVGEYSCVKIPLYSATLFETEETISLSSCTEFIQER
GFFLPALSETLFYYVFTSWGTTLRFSNTKELIDAGLKQFTQDGEQTVKLAPHKTYLGISGQKISAVEKDF
LMLVDSVVTELSFSHVAEYLDSVYDPSQIMNFNDWPIIRNSETHAERMAQLTNLKLHLSSHLAVLIFAPN
SILYCSKLAFIPNVKQAFNSVMTQELLLRSLSFCNALSSLTEDVYNDNRKIIKCDSTSGKDDKFSANHLA
YACATCPQLLSYVVWNLNRMSVYNAGNAYTEIYNHLVNCSANLCEFCDGKCCQSCIGTAMVRVGTRLPAI
PKNVKKEPLVMSMFSRYYAEVDILGSFGRKPVSELKEIGKDQQNTLSLDRGKFVSQIFDYCKKNSLIDPV
TGEDTFNVRSKKDFVSIIHGLTQCIEECVSRCIVEMRRTQTPREQIENCLQSFNVDTTPYATAFSPFLTF
AYYKVILTVLQNLALIVASGHVVDRPCTGNSISKWLVQQYQSLYGTFHSSYLKKGFLNTRTVKVASNVDM
EQILDCDLYKSGKYVKTTIQAKLCRLSMQCLRDFRIKNRPFNKSSKTAHNNPYFKKNVKHKKNPLSGCIS
FLLFKYHDKLFPNVKISCLELWQRFLLNNVPKTLDIGNPEEVKTFIKFAFSITNTYDEIDIIDIQPECLS
TFIDCYFHNKFLSALGFHDYLTSLHGLTSKLVTQNPVLFPVVLDKQPKFSSIQEYLVYVKKLVLDGVPNP
VIASLSKEPNFGTIFTSRSLVTFGLTLEKFVSLANREYFQFGQLGWIGGSGVDRNLNPTSSALQDFRFMR
QKTIIATKFSEVIVKKVRRETIMFDTEVVKGKVLSIVENLTNDIDPELLIIAEVMRDREDKPTMDDMLFF
VDGREALAASIMLKLNHLVDMNVRDFSIANLQSVFEAVSSNDAPVYDFSEILAEEDDQASGVLKCDETET
ETDEPMTKKNRL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 3396 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050222
RefSeq ORF 3396 bp
Locus ID 1497043
MW 127.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...