U73 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101512

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for origin binding protein [Human herpesvirus 6], codon optimized for human cell expression, NP_050252



  View other clones from "Virus" (98)

Need custom modification / cloning service?
Get a free quote

CNY 11,880.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101512 represents NCBI reference of NP_050252 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAACAGGTTCCAGAACGATACGCTGTTCTCAGAATGGTTCGGACAGTCACTGTCAGACGTCAGAT
TCCCTGATAACGTAACCGTTTATTCCCAGGCAGATTCCGCTGTTAGCTTCGAAAACGTGAGACAGCCTAT
CAAGCTGGTCAGAGCCGCAATGGGTTCCGGTAAGACTACTGCCCTGATTCACTTCCTGAAACAGGTGCCA
AAGGAACTTAGCGTGCTGCTCATTAGTTGTCGGAAGACATTTGCTGCCGAGATCCTGCACCGCTTCACTC
TCAACGGTCTCGAGGACTTCGAACTGTATTGTGACATCACAGAAAGGCAGATCAATAACCGCAAAGTAAT
AGTCCAGATCGAGTCTCTGCACCGGCTCACCGAGAACTACGACGTGCTCATACTCGACGAGATTATGAGC
ATTATCAAGCAGTTCTACTCTAAGACAATGACAAAGACAAAGGAAGTGGACTGTAAGTTTTTGTCACTGA
TCAAAAACTCCAGCCACGTGATCGCTATGGACGCAACCCTGACACGGCATGTGGTGGAGTTCTTCGCAGC
CTTTAAGCCAGACACACAGATCGCTCTGATTAGAAATACATTCGTGAGCGCAATGTTCAGTAATCGCGTT
GCCTACTTTTGCGATACGTTCTTTGGCAAAGAGTTCTCATTCTTCGCACGCCTGGAAGACAAGCTCCGCT
GGGATAAAAAGCTCTGTCTCTTTTGCAGCACAGTCCTCGCCGCCGAGTATATGCATGATCTGATTCGCAG
CAGATTTTCTCTGAAAAAAGTGCTGCTGCTGACCTCTAAGCAGGGCAAGTGTTCCAGTATTGAGAGTTGG
ATCCGGTATGACGTGGTGATCTATACCTCAGTCGTGACTGTGGGACTCAGTTTTGAACCCGTGTATTTTA
GCTCCCTGTTCGTGTATATCCAGCTGGCAAAGGGTGGCCCAGACATGGTGTCCATCTTTCAGTCTATTGG
CAGAGTGCGGAGGGTCATCGACGAAGACATCTATATCTATATGAATCCGGTTCTTATCAAGTCATACGAC
CCGCTGGCTCCTATCGCCATGCCCCCATGCTCAGACTGGTCCGTCGCCGAACAGTCTATTATCAGTGAGT
CCTGTATTGACTTCAGAGGAAAATGTAGTGGCGCGCACAAGTACAATTTTTGCAGCGTGCTCAAGTGCCT
TTTTCGGTATCGGCATTACATTGAGAAGACGACCATCACTAGTTTGAGTGATTCTTTGTTTCTGCTGTGT
TCCCTGTTGTGTGAAAACTCCATCAAGGTAGACATTGTCGGTAACGGATTTCCAATGCGGAAGGAAGTGT
TCCTGTCATTTCTGCAAATTTTGGTTGAAGAGTGTCATTTTATTGAGAAAAAAATCACACTTCCTGGAGA
CAACATGACTTTCCAGGAAATAATCTCTTCTAGGGAGACTATTATGAACGGGGACTTTTATGAGAACGGG
AACCAGCTGCTGCACAAGGATTACATTACCGATATGGGCAAATTTAGGGCTACCTTCCTCTCTCCAGGCG
TCGATATTTTCATTGCCTCTGATATCGTCTCCGATCTGAAGAATGAATCAAAGAGATACGTGTTCGTGAA
TGTGTGGCTCCAAAAGTGCGTCAGCGCGGGCGTCGAGTCAACCCGAATCGAGCGGGTGTTTAATGAGCGG
ATCAAAAGCTACGTGTTGCCTAAATCCTTCTTGTGTGACGAGTACTTCGTGCTCGGCGACATTTCCGGTG
TGTACGAGTGGGGCATGCTGATCGACCTTGCATTCCTGGCCGAGATGATCCGGAAGGACTTGAAGCTCAA
GAGCTGCACTGACACTACCACGGACATTAGTGAAGACGACCTGCTGCTCTGCGCCGCCAGGCGCAGCAGC
GATATCCTCCAGATAATGCAGCTTGTGTTCACCGTCCATGTGCAGTTTTTCCAAAGGTATAGCCTCCAAA
CCCTTCAATTGTTCAACAAACTGCGGGGCATGCGGATCGTGACAGGGGTGTTCAGCATAGAGAAGTTCTC
CATAAGTATTTTGCGCCTGTTCTTCAAGTGTGCCTTTAATATGACTCTGTCTGCCTCCAAACCAAGGTAC
ATCCCTGGAAAAGCATATAGGAACCTGACTAAAAATGATCTGGAAAACATGTTGGATAACTGGGAGATCA
GCCGGACAAACCTGAAGACTTGCAAGGAACTTCGGAAAGCCCTGACCGAAGCTAGCAGGGCTCGAAGAAA
GCAGACAATTTACAAACTCCAGGGTAGCGATATCTCACTGTCAGTGTCCGAGGTCGGCGTTTTCGGGCAA
CATGCCTCACCAGGTGTGTGCGTGAGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101512 representing NP_050252
Red=Cloning sites Green=Tags

MENRFQNDTLFSEWFGQSLSDVRFPDNVTVYSQADSAVSFENVRQPIKLVRAAMGSGKTTALIHFLKQVP
KELSVLLISCRKTFAAEILHRFTLNGLEDFELYCDITERQINNRKVIVQIESLHRLTENYDVLILDEIMS
IIKQFYSKTMTKTKEVDCKFLSLIKNSSHVIAMDATLTRHVVEFFAAFKPDTQIALIRNTFVSAMFSNRV
AYFCDTFFGKEFSFFARLEDKLRWDKKLCLFCSTVLAAEYMHDLIRSRFSLKKVLLLTSKQGKCSSIESW
IRYDVVIYTSVVTVGLSFEPVYFSSLFVYIQLAKGGPDMVSIFQSIGRVRRVIDEDIYIYMNPVLIKSYD
PLAPIAMPPCSDWSVAEQSIISESCIDFRGKCSGAHKYNFCSVLKCLFRYRHYIEKTTITSLSDSLFLLC
SLLCENSIKVDIVGNGFPMRKEVFLSFLQILVEECHFIEKKITLPGDNMTFQEIISSRETIMNGDFYENG
NQLLHKDYITDMGKFRATFLSPGVDIFIASDIVSDLKNESKRYVFVNVWLQKCVSAGVESTRIERVFNER
IKSYVLPKSFLCDEYFVLGDISGVYEWGMLIDLAFLAEMIRKDLKLKSCTDTTTDISEDDLLLCAARRSS
DILQIMQLVFTVHVQFFQRYSLQTLQLFNKLRGMRIVTGVFSIEKFSISILRLFFKCAFNMTLSASKPRY
IPGKAYRNLTKNDLENMLDNWEISRTNLKTCKELRKALTEASRARRKQTIYKLQGSDISLSVSEVGVFGQ
HASPGVCVSS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 2340 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050252
RefSeq ORF 2340 bp
Locus ID 1497073
MW 89.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...