U90 (NC_000898) Virus Tagged ORF Clone

CAT#: VC101526

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for IE-A transactivator [Human herpesvirus 6], codon optimized for human cell expression, NP_050266



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CNY 16,340.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101526 represents NCBI reference of NP_050266 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCGCAAAGGATACCACCTCTACTAGTATGTTCATTCTTGGTAAACCCTCCGGGAACAACATGG
AGTCAAACGAGGAGAGAATGCAAAATTATCACCCCGATCCTGTGGTCGAGGAGAGCATAAAGGAAATCTT
GGAGGAAAGTTTGAAGTGTGATGTGTCCTTTGAGTCTCTGCTGTTTCCTGAACTCGAGGCCTTTGACCTC
TTTATCCCAGAGAGTTCCAACGACATTGCAAGTAAAAATGTGAGCTACTCCTCTAACGTCGAGGAGGGGG
CCTCTGATGAATTTAAAACCCTGGTGGCGCAGTCCGTCGGTAACTGCATTCAGTCTATTGGTGCTAGCGT
GAAAGCTGCGATGAAACAAGAACAAAGCAACATGGAGGACAACCTGATCAATTCAGCAGGTCTGCTGACT
CTGCACCGGAGCATGCTGGAGCGACTGGTTCTGGAACAGCTCGGTCAGCTCATAAATATAAATCTGCTGT
CCTCCGCATCAAGTCAATTCGTTTCATGTTACGCGAAAATGCTGAGCGGCAAAAACCTGGATTTTTTCAA
TTGGTGTGAGCCACGATTTATCGTTTTCGCGTGCGACAAATTTGACGGGCTTGTCAAAAAAATTGCATCA
GAGAGCAGGGATCTGCTTATGGACTTGAAAGCAAACATGAATAACCAGTTCATAACAGCACTCAAAAATA
TCTTTTCCAAGGCATACGTGGCCCTGGATTCTGGCAAATTGAATATGGTTGCTACATCCCTTCTGCTGAT
GGCCCATAATAAAGAGATGAGCAATCCTGAAATCTCTAACAAGGAGTTCTGTAAACAGGTGAACCTGCTG
AAGCAAGAGTTGCTTGAGAGTAGAAATGAGATAATAGAGAATCATGTGAAAAACATGAAAATGTTTCAGG
AGTTCGCGAACAAGCAAATGAACCAGATATTTATGGACAACTGTGACAAGACCTTCCTGAAGATCCATAT
CAACTGCAAAAATTTGATTACGGCTGCAAAGAACATAGGCATTGCCGTGCTTCAGTCTATAGTTCTGTGT
TCAAACGAATTCAGTTGGCAGTACCTCAAGCCACGGCGGCATCAGTTTAAGATCACCATGATGAACATGA
TCACGCACGCATGTGAGTGCATTGAAACTATTTACGACGATACCGGACTTATCAAGCCTCTGACTTCATC
TGACATTATGGAAGGATATATAGCCATTAACAAAAATAGGGAATCCTCTATCTGCGACCTGAATATCGAC
CCCTCCGAATCCATACTTCTTGAACTCGCAGATTTTGACGAGCACGGAAAGTATTCCGAGGAATCCAGCA
TCGAGAGCATACATGAGGATGATGACAACGTGGATTACTTGAAATACATGGAGGTCCAGAGCCCCACTGA
TAATAACATTCCTACTCCATCAAAAAACAATGAGTCACCAACACGCCAAAAGCTCACCAACATCCACGAG
AAAGACGTGGGGAAAATGTATCCCGACACTCCATCCCCTGATGTTCCTGGAAAGAGCAAGGAGGCCAAAA
CCTTTATCGAGTACTCTAGGCAAATCGGAAAGGAGCAGACATCCCCTAATTGCGTCTGCACGGCTTCCGT
CACAGATCTCGGAGGCCCTGACAACTTCAAAAGCATCACAGGCTTGGAGTCAGGCAAACACTTCCTGATA
AAAAAGCTTTTGGAGACGCAGCCAGATTCTGTGGTGGTGGAAACCGGCTCAGGCCAGCAGGATATCTTGG
CATATAGCCCTGACAAGAGAAGCCAGACCAAAGAGTGGATTCAGGAAAAAGGTAGTAATTCCAAATGTAC
CGAAACTCTGCCCGGCATGACATTCACTAACTCAGCAACTCCCGTCAAATCCCACGGCGCTATACAGGAT
ACATTGAACCCTGAGAGTAAACTTGACAAGGAAATGGAAGCTGTGGAGAGTTTGGTCAACCTGTGCGATG
GGTTCCACGATAACCCTCTGATTAGTGAGATGATCACCTTTGGTTATGAGACTGATCACAGCGCCCCATA
TGAATCTGAGTCCGACAATAATGACGAGACAGACTACATCGCCGATTGTGATTCAACGGTGAGGACTAAC
AATATACACATGAACAATACGAATGAGAACACCCCATTCAGTAAGTCTCTGTATAGCCCTCCAGAGGTAA
CCCCTAGCAAGGAGGACCACAAAACAGAGAAAATTGTCGCCGTTTCACAAAAATGCAAGAGCAAAAAGCG
CACTGCTAAAAGAAAAAACGTTCCTATTAAACCTAGCAAATCAAAGAAGATCAAACTCGATAGACTGCCC
GAGACAACAAACGTGATCGTGATCAGTAGCGAAAGCGAAGATGAGGAAGATGGAAACAACATAATCGACA
AAAGTATGCTGGAGAAGACTATAAAGAGTGAGCCAAATTCTGAGTCTAGCAGCGAGTCTGACGATTGTAC
CTCAGAGGACAACTACCTCCACCTTAGTGACTACGACAAGGTGATAAATAACGGGCATTGCCAGAGTAAA
GGTTTTCCTAGCCCAGTGTTTACCATTCCTATCCGGAGTATGCCCGGTACGCACGACATTCGCAACAAGT
TTGTGCCAAAGAAACATTGGTTGTGGTTTATGAGAAAGACTCACAAAGTTGACAATTGTGTCATCCATTC
TAGCGCGAAAATGAATGTTAAGAATGATAGCGACGTTACTGAGGCGAACCATTGTTTCATCAATCATTTC
GTGCCTATAAAAACCGATGACGAGGAGTATGAAAAGGAGAACGTGAGCTATACCTACTCCAAGATCCAGG
ATAGCAAAACAGACCTCGAGGATATCACTCCCACAAAGAAGCTGATCACTGAAATGGTCATGGAGAATTT
TATGGACCTGACCGATATCATAAAGCACGGCATCGCCAAACACTGCCAGGATCTGAGTTCCAAATACACC
GTGATCACTCATACCGCCTGCGAAAAGAACCTCAACGTGGCCAACAGTCAAAATCTGGTAACAGCGGAAA
CACAGATCTTTGACCCCCAGGGCACCGGCAATAACAGCCCCATCCTGAATATTATCAATGACACTACTTG
CCAGAACGATGAAAACAGGTGTACGGAAGGCACCTCCAATGATAATGAAAAGTGCACCATCCGGAGCGAC
TGTAATAGCGATAAAATGGAGGTATTCAAGCTCGACGGCTACCCTTCAGATTATGATCCTTTTGAGGAAA
ACGCCCAGATCTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101526 representing NP_050266
Red=Cloning sites Green=Tags

MESAKDTTSTSMFILGKPSGNNMESNEERMQNYHPDPVVEESIKEILEESLKCDVSFESLLFPELEAFDL
FIPESSNDIASKNVSYSSNVEEGASDEFKTLVAQSVGNCIQSIGASVKAAMKQEQSNMEDNLINSAGLLT
LHRSMLERLVLEQLGQLININLLSSASSQFVSCYAKMLSGKNLDFFNWCEPRFIVFACDKFDGLVKKIAS
ESRDLLMDLKANMNNQFITALKNIFSKAYVALDSGKLNMVATSLLLMAHNKEMSNPEISNKEFCKQVNLL
KQELLESRNEIIENHVKNMKMFQEFANKQMNQIFMDNCDKTFLKIHINCKNLITAAKNIGIAVLQSIVLC
SNEFSWQYLKPRRHQFKITMMNMITHACECIETIYDDTGLIKPLTSSDIMEGYIAINKNRESSICDLNID
PSESILLELADFDEHGKYSEESSIESIHEDDDNVDYLKYMEVQSPTDNNIPTPSKNNESPTRQKLTNIHE
KDVGKMYPDTPSPDVPGKSKEAKTFIEYSRQIGKEQTSPNCVCTASVTDLGGPDNFKSITGLESGKHFLI
KKLLETQPDSVVVETGSGQQDILAYSPDKRSQTKEWIQEKGSNSKCTETLPGMTFTNSATPVKSHGAIQD
TLNPESKLDKEMEAVESLVNLCDGFHDNPLISEMITFGYETDHSAPYESESDNNDETDYIADCDSTVRTN
NIHMNNTNENTPFSKSLYSPPEVTPSKEDHKTEKIVAVSQKCKSKKRTAKRKNVPIKPSKSKKIKLDRLP
ETTNVIVISSESEDEEDGNNIIDKSMLEKTIKSEPNSESSSESDDCTSEDNYLHLSDYDKVINNGHCQSK
GFPSPVFTIPIRSMPGTHDIRNKFVPKKHWLWFMRKTHKVDNCVIHSSAKMNVKNDSDVTEANHCFINHF
VPIKTDDEEYEKENVSYTYSKIQDSKTDLEDITPTKKLITEMVMENFMDLTDIIKHGIAKHCQDLSSKYT
VITHTACEKNLNVANSQNLVTAETQIFDPQGTGNNSPILNIINDTTCQNDENRCTEGTSNDNEKCTIRSD
CNSDKMEVFKLDGYPSDYDPFEENAQIY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_000898
ORF Size 3234 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_000898.1, NP_050266
RefSeq ORF 3234 bp
Locus ID 1497087
MW 121.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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