U95 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101436

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for protein U95 [Human herpesvirus 6], codon optimized for human cell expression, NP_042988



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CNY 16,910.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101436 represents NCBI reference of NP_042988 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCTCACCCGGACGATCTTTTCTGGCAGCAGATATTGAATATGGATCCTACAGAGTTGTTGAGTG
ATGATGTGATCAGCAGTACATGTGATGAGAATACTGCAGCAGGGACAGATATCATGCAGCCGCTGTGCGT
GGATATGAGCGTACAGCCTACAACTAGCGGCTGCCATACCGGTGCAATGATGTCTGACAGCTACTTTTCT
AACGACACCCCGGACCAAAATTGTCAAAGTCACTCCACACTCACGGACCTGTCCCCTGAGGACATGAACA
ATCAAATTAATGTGCAACCCAATCAAATGACCTTTCAGCCTATCAGCCCCCCCATTCAGGATCAAAATTA
TGGCTACAGCAACAATATGATAAACCCAATCAAGCCAGCTTCCATAATCAAGCTGCACGGTCACTCTATT
GGTGAGATGACCGTGACCGACCCAAGCAGCCACGTGAATGCACAGCACTTTGTTCAACAGTCAAATCCGA
AATTCTTTCTGAATATGTACCCCTGCATGACTGCCAATAGCCAGGGTCCCGGCGAGTGCCAGAGCGTGCA
CAATCAGTCTAGCGGGTCAGGGAGCAACAGTTACGATATAAACACAGCGCCCAGAGATGAGTGGATATTC
ACTACTAGTGGAGGAGAATCCTGGGTGCTGAAACGGAATACCCCGAATCCACCCAACTCTAGGACAAATC
TGATCTTTAACAATGCCCAGCCCATCTTCCACACCCAGCCCCAGGTCAGCGGCTTTTCCGACTGCAGTTA
TCAGGGCGGCGGCCTGAATTCCCGCGAGTATGTAAACCTCGCCGGCTACACTCCAGTTTGCAACATGCAG
AATATAAAGAATTCCTGGGCTACACAGCTGGGGGCTTCAATTGATTGCATGACCGCTTCTCCCACCGGAA
TTGACGCTAATGTGAACTCTGCTTTCCAGCCGGTAGGCGTAGTTAGCAACAATAGAGGCGCTAATGTCTC
AATGGGCGGTATGGGCAACTATATTGAAAACAACTCACCCTGGAATCAGTACTACAAATCAGAGATGGCA
AACAACTCCATAAATGTCAAGGAAAGCAACGTATACGGTCACGTGGGGTGTGTCAACGACGCAATCAGCG
ACAAACAGGGCGGCACCGCAAATGTGCCATCCTCCCTCCTCAACCCTGACCATCAGGATTGGATGAGGAT
GACCGGAACCAATACTCACATGCTGAACAATATCAACACTAACACCGAAATGGAGAACTACGATTTTCCC
GAGAATGATGGAAACGTGCACGGGGCAACAGGTGTAGATCTTAGTACGCTGTCCAACGGACAGCAGCACA
GCGCTTTCGAGGGCAATGGCACTTTCCCAGTGGTTAATACAGGCAACCCAAGGACCACACTCAATTGCGC
CCCTCAGCTGTTTTTGCTGGGCGGTAACCCTACCATTGCACACCCAATGAATGGCAATAACGAAACTCCC
GACAAAAATGTGTGTAAGCCTACTCCATCTACTGGGGTCATTAAGAAACTGAATTTCGATGGAGTTGATA
GGGGCGGAGGTATTGGCAACTTTCCTAAGCTCGCTAGCTTCGTGGCGATGCGCGAAAAGATGAGCAACAC
AGTTAATCCCTCTTGTGGAACCTTCCTGGAGCAACTTGAAGGCCTGCGACAGCAGAATAGTTTTGAAAAA
AATATAAGCTTTGCCGACCTGCACTTCTCTGAAGAGGATGATGTATTGTCCAGCGCCAGCTCAGTATCAT
GTAACAGTAATTGCGACATGAAGATAGAGGTGTCTCAACAGGGTATTACTGTGAGTAACCTTCAACAGGG
GTTTCGCCAGCAGATGAATATGAATAGCTCCGTGGTTAAAATGGACGGCAGTTACAAGACCCAAGAGATG
TCTAACGGCTGTGCCACCGATGTTACTGATAACGCCGGGACTATTCAGCAGAATAAAAGGATCCACTCCC
AGAACGAAAACCCCGAGGACGGTCAGGTTAGGGAGGGGGGTTGCTCAGATGTCCGCAATGAGCCGCCTAG
AAAGAGTGCCCGCATCCACAACATGAAAAGCGAAGGAGTGACATGCGGCATGTGTATGACCGCTGCCGAT
AGTACCCGACAGGATGCCTCAGGGGGCTCAAGTAGCGGCACCAAAAACGAATATTATGACGACGAGTCCG
AACTGACTGGACTCTCCGACACTGACAGTGATAATGAAGTGCAGTGCTGCCAAGAGGTGACAAAGGTTGG
CCCCAAAACCTACTCCTCCGAAAACTTTAACCCAGAGTATAGGCAGGCTAAGAGGTTGCTGGCCGATATA
CCTTACAGGAGGTGGATCCCCGACCCCGTTAACATGGAGGACAACGATGGCCCCTTTATTCCTATTGTGA
CACGCCCCGCTACGGTGTGTATGAACGGCCGGCGAAGGCGGACTTTCTTTCGCCAGTGTGTGACAGCATT
CGGCCCACTCAGCAAGCTCACTTACTTTAAGGAGCTGCTTCAGTCTTACGTCAGTAAAAACAACAACTCC
TACCTGAGTATCAGTTGGCCTCCAAAGCACGGAGTATACGTCATGTCAGAGAAAAAGCTTGGATACGAAC
ACATCCCTACTCTCAAGGATAAATTCCCGCTTCCATGTGGGTGGATGATGGTGCTCGGTGTTGTGGGCGC
AGAACCCCCGGCGGCCCTGAACAAAAACATGGTGATTCTGTTGTGTGAAAATAGATGGGTCCTGTTGCAC
AACTATTCTAACTCAACTCACGAGCTCTTCTTGGCAGCCTCAGACCTCAAGCAGTTTATGGAGGAGGGGC
TGTCACGCTGTGATCCTATTTACGAAGAATCCACTGTGCCTTACGGGGTGGCTATGGAAAATTCCCTGCG
AGACTTTCTGCGCAACTCTCGGACGTTCCAGGATCTGATGGACCAAAGGGACAATATGCATGGGTGTAAT
TGGACATTTAACGGCATGCCGGGCCGACTGGGCGACAGAGTGATCCACTTGTGCAACCCTGAAAGCGTAG
ACTCTATCCCGGGAGACGAGGCCGTCGTCTGCGAAGGCAGACCACTGTACTTCTTCGCTTATGTGACCAC
TTTTAAGTCCAACCCAGCCACTAAGGCCACAGTCTTGATCGCTGCAGATAAGGATCTGAGAATTTACGGG
TATCACAAAGGTCGACCAAGAATACGATACCTCTGTAAGAACGTGAAAACGTTCTTTAAGGCTGGCGCCC
GCAAATTTTATCTGGATTTTCAAATCACACCGAAACGGCTTCTGGCCGTAAACGAAGAGGAGTATCTGAA
TACCCTGCAGAACGCGCCTTGTCTGCTGTTGAAACCCTCTGTGTTCCGGAACATCTATTCCCAGGAGGGC
AAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101436 representing NP_042988
Red=Cloning sites Green=Tags

MSAHPDDLFWQQILNMDPTELLSDDVISSTCDENTAAGTDIMQPLCVDMSVQPTTSGCHTGAMMSDSYFS
NDTPDQNCQSHSTLTDLSPEDMNNQINVQPNQMTFQPISPPIQDQNYGYSNNMINPIKPASIIKLHGHSI
GEMTVTDPSSHVNAQHFVQQSNPKFFLNMYPCMTANSQGPGECQSVHNQSSGSGSNSYDINTAPRDEWIF
TTSGGESWVLKRNTPNPPNSRTNLIFNNAQPIFHTQPQVSGFSDCSYQGGGLNSREYVNLAGYTPVCNMQ
NIKNSWATQLGASIDCMTASPTGIDANVNSAFQPVGVVSNNRGANVSMGGMGNYIENNSPWNQYYKSEMA
NNSINVKESNVYGHVGCVNDAISDKQGGTANVPSSLLNPDHQDWMRMTGTNTHMLNNINTNTEMENYDFP
ENDGNVHGATGVDLSTLSNGQQHSAFEGNGTFPVVNTGNPRTTLNCAPQLFLLGGNPTIAHPMNGNNETP
DKNVCKPTPSTGVIKKLNFDGVDRGGGIGNFPKLASFVAMREKMSNTVNPSCGTFLEQLEGLRQQNSFEK
NISFADLHFSEEDDVLSSASSVSCNSNCDMKIEVSQQGITVSNLQQGFRQQMNMNSSVVKMDGSYKTQEM
SNGCATDVTDNAGTIQQNKRIHSQNENPEDGQVREGGCSDVRNEPPRKSARIHNMKSEGVTCGMCMTAAD
STRQDASGGSSSGTKNEYYDDESELTGLSDTDSDNEVQCCQEVTKVGPKTYSSENFNPEYRQAKRLLADI
PYRRWIPDPVNMEDNDGPFIPIVTRPATVCMNGRRRRTFFRQCVTAFGPLSKLTYFKELLQSYVSKNNNS
YLSISWPPKHGVYVMSEKKLGYEHIPTLKDKFPLPCGWMMVLGVVGAEPPAALNKNMVILLCENRWVLLH
NYSNSTHELFLAASDLKQFMEEGLSRCDPIYEESTVPYGVAMENSLRDFLRNSRTFQDLMDQRDNMHGCN
WTFNGMPGRLGDRVIHLCNPESVDSIPGDEAVVCEGRPLYFFAYVTTFKSNPATKATVLIAADKDLRIYG
YHKGRPRIRYLCKNVKTFFKAGARKFYLDFQITPKRLLAVNEEEYLNTLQNAPCLLLKPSVFRNIYSQEG
K

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 3363 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042988
RefSeq ORF 3363 bp
Locus ID 1487971
MW 124.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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