U39 (NC_001664) Virus Tagged ORF Clone
CAT#: VC101387
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein B [Human herpesvirus 6], codon optimized for human cell expression, NP_042932
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CNY 12,640.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC101387 represents NCBI reference of NP_042932 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAAATGGCAGTGCTGTTCCTCGCCGTGTTTCTCATGAACAGCGTCCTGATGATTTACTGCGACC CCGACCACTACATTCGGGCCGGTTATAACCACAAGTACCCTTTCCGGATCTGTTCCATCGCCAAAGGTAC AGACCTGATGAGGTTCGATCGGGATATAAGTTGTTCTCCCTACAAGTCTAACGCCAAAATGTCAGAAGGT TTCTTCATCATCTACAAAACCAATATAGAGACATACACATTCCCCGTGAGAACATATAAAAAAGAACTCA CATTTCAAAGTTCATATAGGGACGTTGGCGTTGTATATTTCCTGGACAGAACTGTTATGGGCCTCGCCAT GCCCGTCTATGAGGCAAATCTGGTGAATAGTCACGCTCAGTGCTACTCTGCAGTGGCAATGAAACGCCCT GACGGCACCGTCTTCTCCGCATTCCACGAAGATAACAACAAGAACAACACCCTCAACCTCTTTCCCCTCA ATTTCAAGAGCATAACCAATAAGAGGTTCATAACAACCAAGGAGCCATACTTTGCTCGCGGACCACTGTG GCTGTACAGTACCAGCACATCACTCAATTGTATTGTAACCGAAGCAACCGCTAAGGCCAAGTATCCCTTC AGTTACTTCGCCTTGACTACCGGAGAAATTGTTGAGGGCTCCCCCTTTTTTAATGGATCCAACGGTAAGC ACTTCGCCGAGCCCCTCGAGAAGCTCACCATCCTGGAGAACTACACTATGATCGAGGACCTGATGAACGG TATGAATGGAGCAACCACTCTGGTGCGCAAGATCGCTTTCTTGGAAAAGGCCGATACGCTGTTCAGCTGG GAAATCAAAGAGGAGAACGAGTCTGTCTGCATGCTGAAACATTGGACAACAGTAACCCATGGTCTGCGCG CTGAGACGAATGAGACCTATCATTTCATTAGCAAGGAACTGACCGCCGCATTCGTGGCACCAAAAGAATC TCTTAACCTTACAGACCCGAAACAGACGTGCATAAAGAATGAATTTGAGAAAATCATCAACGAGGTTTAT ATGAGCGATTACAACGACACCTATTCCATGAATGGATCATACCAAATATTCAAAACAACCGGCGACCTGA TTCTGATTTGGCAGCCACTTGTCCAAAAGTCACTCATGTTCCTCGAGCAGGGCAGTGAAAAAATCCGGCG GAGAAGGGACGTGGGCGACGTGAAGTCCAGACATGACATTCTTTACGTTCAGCTTCAGTACCTTTATGAT ACTTTGAAAGACTACATCAATGATGCTCTTGGTAACCTCGCAGAGAGCTGGTGCCTGGATCAGAAGCGAA CGATAACAATGTTGCATGAGCTCTCTAAAATATCTCCCTCCTCTATCGTTAGCGAGGTGTATGGCAGACC CATCAGCGCCCAGCTGCACGGTGATGTCCTGGCGATCTCTAAGTGTATCGAAGTTAACCAGTCAAGTGTC CAATTGCACAAGTCAATGAGAGTGGTGGATGCTAAGGGGGTGAGGTCCGAGACCATGTGCTACAATAGAC CTCTTGTCACCTTTTCTTTCGTTAATTCTACGCCCGAGGTAGTGCCAGGTCAACTTGGACTGGACAACGA GATCCTTCTCGGCGACCACCGGACAGAAGAATGTGAGATTCCAAGCACCAAGATCTTTCTGTCTGGGAAT CACGCCCACGTCTATACAGACTATACTCACACCAATTCCACCCCCATCGAAGATATCGAGGTCCTGGACG CATTCATTCGACTGAAAATTGATCCTTTGGAGAATGCCGACTTCAAGGTTCTGGACTTGTATAGTCCCGA CGAACTCAGCCGCGCTAACGTGTTTGACTTGGAGAACATCCTTCGAGAATATAACAGCTATAAGTCCGCC CTTTACACGATCGAGGCCAAGATTGCCACTAATACACCCTCTTACGTTAACGGAATAAATAGTTTCCTCC AGGGTCTCGGCGCCATAGGGACCGGTCTGGGAAGTGTGATCAGCGTGACCGCTGGCGCCCTGGGGGACAT TGTGGGTGGCGTGGTGTCTTTCCTTAAGAACCCTTTTGGTGGTGGGCTGATGCTGATCCTGGCGATTGTG GTAGTCGTAATTATTATCGTCGTTTTTGTGCGGCAGCGGCACGTCTTGAGCAAACCCATAGACATGATGT TCCCTTATGCGACCAACCCCGTTACTACCGTCTCCTCCGTAACCGGCACGACGGTTGTGAAGACCCCATC CGTGAAAGATGTCGATGGTGGCACCTCAGTTGCCGTGTCAGAGAAAGAAGAAGGAATGGCCGACGTGAGT GGACAGGTCTCAGATGATGAGTATAGTCAGGAGGACGCATTGAAGATGCTTAAGGCTATTAAGAGCCTGG ATGAGAGCTACCGCCGAAAACCCAGCTCTTCTGAAAGTCACGCATCTAAGCCCAGTCTTATCGATCGCAT TCGGTACCGGGGCTATAAGAGTGTGAATGTTGAGGAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101387 representing NP_042932
Red=Cloning sites Green=Tags MSKMAVLFLAVFLMNSVLMIYCDPDHYIRAGYNHKYPFRICSIAKGTDLMRFDRDISCSPYKSNAKMSEG FFIIYKTNIETYTFPVRTYKKELTFQSSYRDVGVVYFLDRTVMGLAMPVYEANLVNSHAQCYSAVAMKRP DGTVFSAFHEDNNKNNTLNLFPLNFKSITNKRFITTKEPYFARGPLWLYSTSTSLNCIVTEATAKAKYPF SYFALTTGEIVEGSPFFNGSNGKHFAEPLEKLTILENYTMIEDLMNGMNGATTLVRKIAFLEKADTLFSW EIKEENESVCMLKHWTTVTHGLRAETNETYHFISKELTAAFVAPKESLNLTDPKQTCIKNEFEKIINEVY MSDYNDTYSMNGSYQIFKTTGDLILIWQPLVQKSLMFLEQGSEKIRRRRDVGDVKSRHDILYVQLQYLYD TLKDYINDALGNLAESWCLDQKRTITMLHELSKISPSSIVSEVYGRPISAQLHGDVLAISKCIEVNQSSV QLHKSMRVVDAKGVRSETMCYNRPLVTFSFVNSTPEVVPGQLGLDNEILLGDHRTEECEIPSTKIFLSGN HAHVYTDYTHTNSTPIEDIEVLDAFIRLKIDPLENADFKVLDLYSPDELSRANVFDLENILREYNSYKSA LYTIEAKIATNTPSYVNGINSFLQGLGAIGTGLGSVISVTAGALGDIVGGVVSFLKNPFGGGLMLILAIV VVVIIIVVFVRQRHVLSKPIDMMFPYATNPVTTVSSVTGTTVVKTPSVKDVDGGTSVAVSEKEEGMADVS GQVSDDEYSQEDALKMLKAIKSLDESYRRKPSSSESHASKPSLIDRIRYRGYKSVNVEEA TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001664 |
ORF Size | 2490 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001664.2, NP_042932 |
RefSeq ORF | 2490 bp |
Locus ID | 1487917 |
MW | 93.2 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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VC101369 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein U22 [Human herpesvirus 6], codon optimized for human cell expression, NP_042915 |
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VC101370 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein U23 [Human herpesvirus 6], codon optimized for human cell expression, NP_042916 |
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VC101372 | Myc-DDK-tagged ORF clone of viral ORF for protein U24A [Human herpesvirus 6], codon optimized for human cell expression, YP_002608174 |
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VC101376 | Myc-DDK-tagged ORF clone of viral ORF for ribonucleotide reductase subunit 1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042921 |
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VC101377 | Myc-DDK-tagged ORF clone of viral ORF for capsid triplex subunit 1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042922 |
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VC101378 | Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 6], codon optimized for human cell expression, NP_042923 |
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VC101381 | Myc-DDK-tagged ORF clone of viral ORF for protein UL49 [Human herpesvirus 6], codon optimized for human cell expression, NP_042926 |
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VC101382 | Myc-DDK-tagged ORF clone of viral ORF for nuclear egress membrane protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042927 |
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VC101383 | Myc-DDK-tagged ORF clone of viral ORF for DNA packaging protein UL33 [Human herpesvirus 6], codon optimized for human cell expression, NP_042928 |
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VC101384 | Myc-DDK-tagged ORF clone of viral ORF for DNA packaging protein UL32 [Human herpesvirus 6], codon optimized for human cell expression, NP_042929 |
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VC101386 | Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042931 |
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VC101389 | Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042934 |
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VC101390 | Myc-DDK-tagged ORF clone of viral ORF for multifunctional expression regulator [Human herpesvirus 6], codon optimized for human cell expression, NP_042935 |
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VC101391 | Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042936 |
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VC101394 | Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein N [Human herpesvirus 6], codon optimized for human cell expression, NP_042939 |
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VC101395 | Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein O [Human herpesvirus 6], codon optimized for human cell expression, NP_042940 |
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VC101412 | Myc-DDK-tagged ORF clone of viral ORF for protein UL92 [Human herpesvirus 6], codon optimized for human cell expression, NP_042956 |
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VC101413 | Myc-DDK-tagged ORF clone of viral ORF for DNA packaging tegument protein UL17 [Human herpesvirus 6], codon optimized for human cell expression, NP_042957 |
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VC101414 | Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL16 [Human herpesvirus 6], codon optimized for human cell expression, NP_042958 |
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VC101417 | Myc-DDK-tagged ORF clone of viral ORF for tegument serine/threonine protein kinase [Human herpesvirus 6], codon optimized for human cell expression, NP_042962 |
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VC101418 | Myc-DDK-tagged ORF clone of viral ORF for deoxyribonuclease [Human herpesvirus 6], codon optimized for human cell expression, NP_042963 |
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VC101421 | Myc-DDK-tagged ORF clone of viral ORF for DNA replication origin-binding helicase [Human herpesvirus 6], codon optimized for human cell expression, NP_042966 |
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VC101424 | Myc-DDK-tagged ORF clone of viral ORF for capsid portal protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042969 |
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VC101430 | Myc-DDK-tagged ORF clone of viral ORF for protein UL117 [Human herpesvirus 6], codon optimized for human cell expression, NP_042977 |
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VC101431 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein UL119 [Human herpesvirus 6], codon optimized for human cell expression, NP_042978 |
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VC101433 | Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042983 |
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VC101434 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein UL124 [Human herpesvirus 6], codon optimized for human cell expression, NP_042984 |
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VC101435 | Myc-DDK-tagged ORF clone of viral ORF for protein U94/rep [Human herpesvirus 6], codon optimized for human cell expression, NP_042987 |
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VC101436 | Myc-DDK-tagged ORF clone of viral ORF for protein U95 [Human herpesvirus 6], codon optimized for human cell expression, NP_042988 |
CNY 16,910.00 |
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VC101437 | Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein Q [Human herpesvirus 6], codon optimized for human cell expression, NP_042993 |
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VC101438 | Myc-DDK-tagged ORF clone of viral ORF for protein DR1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042995 |
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VC101439 | Myc-DDK-tagged ORF clone of viral ORF for US22 family [Human herpesvirus 6], codon optimized for human cell expression, NP_043000 |
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