U41 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101389

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042934



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CNY 17,100.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101389 represents NCBI reference of NP_042934 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGACGAGAACGAAACTGTGGTTAGCGCGCCAGTCAGCACTGCCGCTTGGATATACGTTTTCCCGA
AAGATAAGGAGCTCCTGGACGTCCTGAGTGTGCTGAGCCTTATGGAACGCAACAGCCCCATCGTCATCTC
TCCACTCCTCATGAATCTCACAGTGGAAAACGACTTTTCCACAACAGTTAAAACCCCCATTACCAACTTC
GGAGGCACAATCCTGACTAAGATTACTAGCTTCATGCCAGTCTGTTTTTTTTTCCATGGGACCGAGCAAC
TCGTTGGGATGGCAGAGGATCATGGGGATCTTATCAGGCTGTGTGAGCAGACCCGCCAGAAGTTCCACCT
CCAGTCCTTCGAGGTCCCGACAGCTAGAAAGGTCATCGATATCAAAGCACTTTGCTCTGCAGTAGGGAAG
GACGCCGATTCAGTAATCTGTCATGTCGCTTGTGGAAATGGGTTTAAAGAACTGCTGTTCGCCGGCCTGC
TCATACCTTGTGTAGAAGAGCAGATCCAAGTGCAAGTGGGCGAATACAGCTGCGTGAAAATCCCTCTGTA
CTCAGCTACCCTCTTCGAAACCGAGGAGACCATTAGTCTCAGTAGCTGCACCGAGTTTATCCAGGAGAGG
GGATTTTTTTTGCCGGCCCTTTCAGAAACTCTCTTCTATTACGTGTTTACAAGCTGGGGCACGACTCTGA
GGTTTAGCAACACGAAGGAACTTATTGACGCGGGCCTGAAGCAGTTTACTCAGGATGGAGAGCAAACAGT
CAAGCTCGCACCCCACAAAACTTATTTGGGCATTAGTGGGCAGAAGATATCAGCCGTGGAGAAGGATTTC
CTTATGCTCGTGGACAGCGTCGTCACAGAACTCTCTTTTTCTCACGTAGCTGAGTACCTCGACAGCGTCT
ACGACCCCTCCCAGATTATGAACTTTAACGATTGGCCCATTATTCGGAATAGTGAAACACACGCTGAAAG
AATGGCCCAGCTCACTAATCTGAAGCTGCACCTCTCATCCCATTTGGCTGTTCTGATATTCGCCCCAAAC
AGTATACTGTATTGCTCCAAGCTGGCTTTCATCCCAAACGTGAAGCAGGCGTTCAATAGCGTAATGACCC
AAGAGCTGCTGCTTCGGTCACTGTCTTTCTGCAATGCCCTGAGCAGTCTGTCCGACGATGTTTATAACGA
TAACCGGAAAATCATTAAATGTGACTCTACTAGTGGCAAGGATGACAAATTCAGCGCTAATCATCTTGCC
TACGCCTGTGCAACCAGTCCGCAACTCCTGTCCTTCGTTGTTTGGAATTTGAACCGGATGTCTGTCTACA
ACGCCGGTAACGCACACACGGAGATCTACAACCACCTCGTCAACTGCTCCGCAAACCTCTGTGAATTCTG
CGATGGAAAGTGTTGTCAGAGCTGTATTGGAACCGCCATGGTAAGAGTGGGCACCAGGCTGCCCGCAATC
CCCAAGAACGTGAAAAAGGAGCCGCTTGTGATGAGCATGTTTTCTAGGTATTATGCCGAGGTGGACATCC
TGGGCTCCTTCGGGCGAAAACCTGTATCTGAGTTGAAAGAGATCGGAAAGGACCAGCAGAATACCCTGTC
CCTGGATAGAGGCAAATTCGTCTCACAGATCTTCGACTACTGTAAGAAAAACTCACTGATCGATCCTGTT
ACCGGCGAAGACACTTTCAATGTCCGCTCAAAAAAGGACTTCGTGTCAATCATCCATGGCCTGACTCAGT
GTATCGAAGAATGTGTGTCCAGGTGCATAGTTGAGATGCGCCGGACACAGACGCCAAGAGAACAAATCGA
GAACTGTCTGCAGTCATTCAATGTAGATACTACGCCGTACGCCACCGCCTTTTCCCCCTTCCTCACATTC
TCCTACTATAAGGTGATCCTGACCGTGCTTCAGAATCTGGCCCTTATAGTCGCATCTGGCCATGTGGTCG
ATAGACCTTGTACAGGAAACAGCATTAGTAAGTGGCTGGTGCAGCAGTACCAATCCCTGTACGGCACCTT
TCACAGCAGCTATCTGAAAAAAGGGTTTCTCAATACACGCACTGTGAAGGTCGCTAGCAATGTGGACATG
GAGCAGATTCTCGACTGCGATCTGTATAAATCCGGTAAGTATGTTAAAACTACAATCCAGGCCAAGTTGT
GCAGGCTGTCCATGCAATGCTTGAGAGACTTTAGAATTAAAAACAGGCCCTTTAATAAATCTTCAAAGAC
AGCTCATAATAATCCTTACTTCAAAAAGAACGTGAAGCACAAGAAGAACCCACTCAGTGGTTGCATCTCT
TTCCTCCTGTTTAAATACCATGACAAACTGTTCCCCAATGTGAAAATATCTTGCTTGGAGCTTTGGCAAA
GATTTCTGCTGAACAACGTTCCAAAAACTCTCGATATTGGCAACCCAGAGGAGGTAAAGACTTTTATCAA
GTTCGCATTCAGCATAACGAATACCTACGATGAGATTGACATTATCGATATCCAGCCCGAATGCTTGAGC
ACCTTCATCGATTGTTATTTCCACAATAAATTCCTGTCCGCACTGGGTTTTCACGACTACTTGACTAGTC
TTCACGGTCTGACTAGCAAACTGGTTACCCAAAACCCCGTGCTGTTCCCAGTGGTCCTTGATAAGCAGCC
GAAATTCTCTTCCATACAGGAGTATCTCGTATATGTCAAGAAGTTGGTGCTTGATGGCGTTCCAAATCCC
GTAATCGCTTCTCTCTCCAAAGAACCCAATTTTGGTACAATCTTTACTAGCCGGTCACTGGTCACTTTCG
GTCTGACCCTGGAAAAGTTCGTTAGCCTGGCAAACAGAGAATATTTCCAATTTGGCCAGTTGGGGTGGAT
CGGGGGTTCTGGGGTCGATAGGAATCTGAACCCAACAAGCAGCGCATTGCAAGATTTTCGGTTCATGAGA
CAGAAAACTATTATCGCTACGAAATTTAGTGAGGTAATCGTTAAAAAAGTACGGCGGGAAGCCATTATGT
TCGACACCGAGGTGGTTAAAGGAAAAGTGCTCTCCATCGTGGAAAATCTGACTAACGACATTGACCCAGA
ATTGCTGATCATTGCTGAGGTTATGAGAGACAGAGAGGACAAGCCCACCATGGACGACATGCTGTTCTTC
GTGGATGGACGAGAGGCCCTGGCCGCCTCTATCATGCTGAAGTTGAACCACCTCGTCGATATGAACGTGA
AGGACTTTTCCATTACTAACCTCCAAAGCGTCTTCGAAACAGTTTCTTCTAACGACGCGCCTGTTTACGA
CTTCTCCGAGATCCTGGCCGAAGAAGATGACCAGGGCAATGGAGTGCTTAAGTGTGATGAAACCGAGACT
GAGACAGATGAACCTATGACCAAGAAAAATCGGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101389 representing NP_042934
Red=Cloning sites Green=Tags

MADENETVVSAPVSTAAWIYVFPKDKELLDVLSVLSLMERNSPIVISPLLMNLTVENDFSTTVKTPITNF
GGTILTKITSFMPVCFFFHGTEQLVGMAEDHGDLIRLCEQTRQKFHLQSFEVPTARKVIDIKALCSAVGK
DADSVICHVACGNGFKELLFAGLLIPCVEEQIQVQVGEYSCVKIPLYSATLFETEETISLSSCTEFIQER
GFFLPALSETLFYYVFTSWGTTLRFSNTKELIDAGLKQFTQDGEQTVKLAPHKTYLGISGQKISAVEKDF
LMLVDSVVTELSFSHVAEYLDSVYDPSQIMNFNDWPIIRNSETHAERMAQLTNLKLHLSSHLAVLIFAPN
SILYCSKLAFIPNVKQAFNSVMTQELLLRSLSFCNALSSLSDDVYNDNRKIIKCDSTSGKDDKFSANHLA
YACATSPQLLSFVVWNLNRMSVYNAGNAHTEIYNHLVNCSANLCEFCDGKCCQSCIGTAMVRVGTRLPAI
PKNVKKEPLVMSMFSRYYAEVDILGSFGRKPVSELKEIGKDQQNTLSLDRGKFVSQIFDYCKKNSLIDPV
TGEDTFNVRSKKDFVSIIHGLTQCIEECVSRCIVEMRRTQTPREQIENCLQSFNVDTTPYATAFSPFLTF
SYYKVILTVLQNLALIVASGHVVDRPCTGNSISKWLVQQYQSLYGTFHSSYLKKGFLNTRTVKVASNVDM
EQILDCDLYKSGKYVKTTIQAKLCRLSMQCLRDFRIKNRPFNKSSKTAHNNPYFKKNVKHKKNPLSGCIS
FLLFKYHDKLFPNVKISCLELWQRFLLNNVPKTLDIGNPEEVKTFIKFAFSITNTYDEIDIIDIQPECLS
TFIDCYFHNKFLSALGFHDYLTSLHGLTSKLVTQNPVLFPVVLDKQPKFSSIQEYLVYVKKLVLDGVPNP
VIASLSKEPNFGTIFTSRSLVTFGLTLEKFVSLANREYFQFGQLGWIGGSGVDRNLNPTSSALQDFRFMR
QKTIIATKFSEVIVKKVRREAIMFDTEVVKGKVLSIVENLTNDIDPELLIIAEVMRDREDKPTMDDMLFF
VDGREALAASIMLKLNHLVDMNVKDFSITNLQSVFETVSSNDAPVYDFSEILAEEDDQGNGVLKCDETET
ETDEPMTKKNRL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 3396 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042934
RefSeq ORF 3396 bp
Locus ID 1487919
MW 127.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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