U47 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101395

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein O [Human herpesvirus 6], codon optimized for human cell expression, NP_042940



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CNY 7,410.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101395 represents NCBI reference of NP_042940 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCACATCAGCCGCCTCGGACTCTTTCTGGGACTGTTCGCCATCGTGATGCATAGCGTAAACTTGA
TCAAATATACTAGCGATCCCCTTGAAGCCTTTAAGACAGTTAATCGCCATAACTGGTCAGATGAGCAGAG
GGAACACTTCTACGATCTGCGAAACCTCTACACTTCCTTCTGCCAAACTAACCTTTCCCTCGATTGTTTT
ACTCAGATCTTGACGAACGTGTTCTCTTGGGACATTCGCGACTCTCAGTGTAAAAGTGCTGTCTCTCTCT
CCCCACTCCAAAATTTGCCCCGCACAGAAATAAAAATCGTGCTCTCATCTACTACAGCAAACAAATCTAT
CATCGCTTCTAGTTTCAGCCTTTTTTACCTGCTCTTCGCCACCCTGTCCACCTATACAGCAGATCCTCCG
TGCGTTGAACTGCTGCCCTTCAAGATTTTGGGCGCTCAGCTTTTCGATATCAAGCTTACTGAGGAGTCTC
TGCGCATGGCCATGAGCAAATTCTCTAACTCCAACTTGACGCGGAGTCTGACCTCCTTTACTTCTAAAAA
TTTCTTCAATTATACCAGCTTTGTTTATTTTCTGCTTTACAATACTACATCTTGCGTCCCGAGTAATGAT
CAATACTTCAAGCAGTCACCAAAGCCTATTAACGTGACGACGAGTTTTGGCAGGGCCATCGTGAACTTCG
ATAGCATTCTGACCACGACGCCCAGCAGCACATCAGCGTCTTTGACGTCACCCCATATCCCATCTACAAA
CATACCCACCCCCGCCCCTCCTCCAGTGACAAAAAACAGCACTAAACTTCATACCGACACTATTAAGGTC
ACCCCCAACACACCTACGATAACGACCCAGACAACCGAAAGCATCAAAAAAATTGTGAAGCGCAGCGATT
TCCCCCGCCCGATGTATACCCCAACAGACATTCCTACCCTTACAATCAGGCTGAACGCTACTATTAAGAC
TGAACAGAATACAGAGAATCCGAAGAGCCCCCCTAAGCCAACTAATTTCGAAAATACAACTATAAGAATC
CCCAAGACCCTCGAGAGCGCTACAGCCACTACCAATGCTACACAGAAAATCGAGTCAACAACTTTCACCA
CTATTGGTATAAAGGAGATCAATGGTAATACATATAGCAGCCCAAAGAATAGCATTTATCTTAAATCCAA
ATCACAGCAGAGCACCACTAAGTTTACCGACGCGGAACATACGACACCGATCTTGAAGTTTACAACTTGG
CAGAACACAGTGCGCACTTACATGTCTCACAACACCGAAGTTCAGAATATGACCGATAAGTTCCAGCGAA
CAACCCTTAAGAGCTCCAATGAACTGCCTACAATCCAGACACTGTCTGTGACACCCAAGCAAAAACTGCC
CTCCAACGTCACTGCAAAGACCGAAGTACACATCACTAACAACGCCCTGCCAAGCTCCAACAGCAGTTAC
AGCATTACCGAGGTCACTAAGGAAGTGAAGCACACTAGGATGTCTGCCAGCACCCATGAACAAATTAATC
ATACTGAGATCGCTCAGATCACCCCAATCCTCAACGCCCATACCTCCGAAAAGTCTACGACCCCCCAAAG
GTCCTTCACAGCCGAGACATTCCTTACAACATCATCAAAGCCAAATATCATTACATGGTCTAATCTTCTT
ACCACTACACCCAAAGAACCCCTGACGAATACCAGTCTCAGGTGGACTGATCATATAACAACTCAGTTGA
CTACTTCAAACCGCACCCAAAGCGCCAAACTTACGAAGGCAAATATCTCATCTCAGACGACCAATATATA
TCCTCAAACCATCACTGGCCGAAGTACAGAAGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101395 representing NP_042940
Red=Cloning sites Green=Tags

MLHISRLGLFLGLFAIVMHSVNLIKYTSDPLEAFKTVNRHNWSDEQREHFYDLRNLYTSFCQTNLSLDCF
TQILTNVFSWDIRDSQCKSAVSLSPLQNLPRTEIKIVLSSTTANKSIIASSFSLFYLLFATLSTYTADPP
CVELLPFKILGAQLFDIKLTEESLRMAMSKFSNSNLTRSLTSFTSKNFFNYTSFVYFLLYNTTSCVPSND
QYFKQSPKPINVTTSFGRAIVNFDSILTTTPSSTSASLTSPHIPSTNIPTPAPPPVTKNSTKLHTDTIKV
TPNTPTITTQTTESIKKIVKRSDFPRPMYTPTDIPTLTIRLNATIKTEQNTENPKSPPKPTNFENTTIRI
PKTLESATATTNATQKIESTTFTTIGIKEINGNTYSSPKNSIYLKSKSQQSTTKFTDAEHTTPILKFTTW
QNTVRTYMSHNTEVQNMTDKFQRTTLKSSNELPTIQTLSVTPKQKLPSNVTAKTEVHITNNALPSSNSSY
SITEVTKEVKHTRMSASTHEQINHTEIAQITPILNAHTSEKSTTPQRSFTAETFLTTSSKPNIITWSNLL
TTTPKEPLTNTSLRWTDHITTQLTTSNRTQSAKLTKANISSQTTNIYPQTITGRSTEV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042940
RefSeq ORF 1854 bp
Locus ID 1487926
MW 69.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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