U27 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101375

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase processivity subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042920



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CNY 4,370.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

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    • 5 x 200 ul

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101375 represents NCBI reference of NP_042920 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAGGGTCAAGAGATCACCACCGCGACCACAGAGATCACCGGGAGCATAGGGAGACACGCGAGC
CCCCCACGCTGGCCTTTCACATGAAAAGCTGGAAGACTATTAACAAAAGCCTGAAGGCGTTTGCAAAGCT
TCTCAAAGAAAATACCACAGTAACGTTCACGCCGCAACCCTCCATCATTATTCAATCTGCCAAGAACCAT
CTTGTGCAAAAGCTTACGATTCAGGCCGAGTGTCTTTTCCTCAGTGACACGGATCGCTTCCTGACCAAAA
CCATCAATAACCACATTCCACTGTTTGAAAGTTTTATGAACATTATCTCAAATCCAGAGGTGACCAAAAT
GTATATCCAGCATGACAGTGATTTGTACACACGGGTTCTCGTGACTGCGTCCGATACATGCACCCAGGCA
TCTGTCCCCTGCGTACATGGTCAGGAGGTCGTGCGGGACACAGGCCGCAGTCCGTTGCGGATCGACCTGG
ATCACAGCACTGTGTCTGACGTGTTGAAATGGCTGAGTCCCGTGACCAAGACGAAACGCTCCGGAAAGAG
CGATGCGCTGATGGCACACATCATTGTGCAAGTCAACCCACCCACTATTAAATTTGTGACCGAGATGAAC
GAACTTGAATTCTCCAATTCAAATAAAGTCATCTTCTACGATGTGAAGAATATGAGATTCAACCTTAGCG
CAAAGAACCTTCAGCAAGCTCTCTCTATGTGTGCCGTAATAAAGACCAGCTGTAGCCTGAGGACGGTAGC
AGCCAAGGATTGTAAGTTGATTCTGACCTCTAAGTCAACTCTGTTGACAGTCGAGGCCTTCCTGACACAA
GAGCAACTTAAGGAGGAGAGTAGATTTGAACGGATGGGAAAACAAGATGACGGGAAGGGAGATCGCTCCC
ACAAAAATGACGATGGATCTGCCCTGGCATCCAAACAGGAGATGCAGTATAAAATCACTAATTATATGGT
GCCGGCAAAAAATGGAACTGCCGGCTCCAGTCTGTTTAATGAGAAGGAGGATAGTGAATCCGACGACTCA
ATGCATTTTGATTACAGCAGTAACCCCAACCCCAAGCGCCAGCGGTGCGTAGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101375 representing NP_042920
Red=Cloning sites Green=Tags

MERGSRDHHRDHRDHREHRETREPPTLAFHMKSWKTINKSLKAFAKLLKENTTVTFTPQPSIIIQSAKNH
LVQKLTIQAECLFLSDTDRFLTKTINNHIPLFESFMNIISNPEVTKMYIQHDSDLYTRVLVTASDTCTQA
SVPCVHGQEVVRDTGRSPLRIDLDHSTVSDVLKWLSPVTKTKRSGKSDALMAHIIVQVNPPTIKFVTEMN
ELEFSNSNKVIFYDVKNMRFNLSAKNLQQALSMCAVIKTSCSLRTVAAKDCKLILTSKSTLLTVEAFLTQ
EQLKEESRFERMGKQDDGKGDRSHKNDDGSALASKQEMQYKITNYMVPAKNGTAGSSLFNEKEDSESDDS
MHFDYSSNPNPKRQRCVV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042920
RefSeq ORF 1104 bp
Locus ID 1487904
MW 41.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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