U54 (NC_001664) Virus Tagged ORF Clone
CAT#: VC101404
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp65 [Human herpesvirus 6], codon optimized for human cell expression, NP_042947
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CNY 4,024.00
Cited in 1 publication. |
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>VC101404 representing NP_042947.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGCCCGCTACACTCCAATGGTCCTCCTATGTGCTTCAGTTGAGAGTGACCACCACCGCGATACTT AAACCCGGCGAACTCCGCTTGTTTAAATGCGGATTGGGCATCACGCCACCTTCCAGTGCCGTTGTTTGC ATTTGTCGAGATGAATCTTCCTTCACTTCCAGTCCGTTCACGTATATTGACCCGAAGGACTATGGCAAT ATCCCATTCGCAGTGCAGAATATCAGCGACCTCGATCTCGACTTGTCTCGGCTTCCCATTGTTCTCAAC ATCTTCGCCTTGCCCCATGCCAATGTTAATATCTCCAATTTGCCAGTGCAGCGAATTGAGGCCCATGAC AGACATATCATCCCACATGGGCAGTGTGACGCCAAATTCGTGATCTATGGACCTCTGACTCGCATTAAA ATACAGGTGGCAGATATCCGATGGATCGAGCAGACACCCGAAGAGCCAACGAGGTACAGTTTTAAAGCA GAGATCTGGGTGAACCTCCAAAACACGCCTCTTGACCAGATCTTTAAGACCGGGAACATAGAGTTCATC AGTCATAAGAATGTATACATATCAAGAATCCTCCTTTGCGGCAACCTCCTTATCCTGAAGGCCAATTAC GAAAACGACTATCTCCTGGACGATAACTTTTACCCAGAACAGCTGGCCATCCAAATTAGCCTGACCCCA CAGACCGCCGACATAACGTTGAGTCAGAATCAGGAAACATTGCTTAAATGCAACACCCATAGCATCACC GTGTGTGCTACTAAGAACATCATACCGAATACTGTTACACCTGTTCACTGCTCCTTCAACACAATCTTC AATTCCAACAGCAACTTCACAGGCCTGTTTATACCAAAGCTCCTGCTGGGCATCAGCACGACGACAGGA ATTTGGGATGAGACACGGCCGCTCTATATAACCATGAAAGCTTTCAAGAAAAATAGGCGCATCAATTAT TCACAGCCGATCGGTGTTGTTTACTTTTTTCCTAAACAGATCTTGCCACCAGGAAACAACGTTGAGTTC AATTGGACAGAAGCCTCCAAAATTTACGTTAATACAGAGTCACCCAACGGGCCTGTCCGCAACACAGTG ACATTCACAAACCAGGCCGTGCTTCGCACCCCCAGCCTGAGCACGGTTGCCAACCTGACTCCTGACATA AACATGGGAATATTCCTCTCAAGCCTCAGGGTGGCCTTTGACACTGCGCACATGGTTCCTCTGCACTTT AGCCTGAAGCCAGGAGAGTCTACTAGGATGGAATTCATGCCCCCTGGGACCCCTCAGAATCTGACCATT CTCGAGGGCGATGTTGGTATCCACTTCATACCCTGTCACAACCACTCACATCGGTCCTCTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >VC101404 representing NP_042947
Blue=ORF Red=Cloning site Green=Tag(s) MQPATLQWSSYVLQLRVTTTAILKPGELRLFKCGLGITPPSSAVVCICRDESSFTSSPFTYIDPKDYGN IPFAVQNISDLDLDLSRLPIVLNIFALPHANVNISNLPVQRIEAHDRHIIPHGQCDAKFVIYGPLTRIK IQVADIRWIEQTPEEPTRYSFKAEIWVNLQNTPLDQIFKTGNIEFISHKNVYISRILLCGNLLILKANY ENDYLLDDNFYPEQLAIQISLTPQTADITLSQNQETLLKCNTHSITVCATKNIIPNTVTPVHCSFNTIF NSNSNFTGLFIPKLLLGISTTTGIWDETRPLYITMKAFKKNRRINYSQPIGVVYFFPKQILPPGNNVEF NWTEASKIYVNTESPNGPVRNTVTFTNQAVLRTPSLSTVANLTPDINMGIFLSSLRVAFDTAHMVPLHF SLKPGESTRMEFMPPGTPQNLTILEGDVGIHFIPCHNHSHRSSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NC_001664 |
ORF Size | 1374 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NP_042947 |
RefSeq ORF | 1374 bp |
Locus ID | 1487936 |
MW | 51.5 kDa |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRaß+ CD8aß+ T cells that can be targeted for cancer immunotherapy
,Komdeur, FL;Wouters, MC;Workel, HH;Tijans, AM;Terwindt, AL;Brunekreeft, KL;Plat, A;Klip, HG;Eggink, FA;Leffers, N;Helfrich, W;Samplonius, DF;Bremer, E;Wisman, GB;Daemen, T;Duiker, EW;Hollema, H;Nijman, HW;de Bruyn, M;,
Oncotarget
,PubMed ID 27650547
[U54]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
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