U54 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101404

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp65 [Human herpesvirus 6], codon optimized for human cell expression, NP_042947



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CNY 4,024.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>VC101404 representing NP_042947.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAGCCCGCTACACTCCAATGGTCCTCCTATGTGCTTCAGTTGAGAGTGACCACCACCGCGATACTT
AAACCCGGCGAACTCCGCTTGTTTAAATGCGGATTGGGCATCACGCCACCTTCCAGTGCCGTTGTTTGC
ATTTGTCGAGATGAATCTTCCTTCACTTCCAGTCCGTTCACGTATATTGACCCGAAGGACTATGGCAAT
ATCCCATTCGCAGTGCAGAATATCAGCGACCTCGATCTCGACTTGTCTCGGCTTCCCATTGTTCTCAAC
ATCTTCGCCTTGCCCCATGCCAATGTTAATATCTCCAATTTGCCAGTGCAGCGAATTGAGGCCCATGAC
AGACATATCATCCCACATGGGCAGTGTGACGCCAAATTCGTGATCTATGGACCTCTGACTCGCATTAAA
ATACAGGTGGCAGATATCCGATGGATCGAGCAGACACCCGAAGAGCCAACGAGGTACAGTTTTAAAGCA
GAGATCTGGGTGAACCTCCAAAACACGCCTCTTGACCAGATCTTTAAGACCGGGAACATAGAGTTCATC
AGTCATAAGAATGTATACATATCAAGAATCCTCCTTTGCGGCAACCTCCTTATCCTGAAGGCCAATTAC
GAAAACGACTATCTCCTGGACGATAACTTTTACCCAGAACAGCTGGCCATCCAAATTAGCCTGACCCCA
CAGACCGCCGACATAACGTTGAGTCAGAATCAGGAAACATTGCTTAAATGCAACACCCATAGCATCACC
GTGTGTGCTACTAAGAACATCATACCGAATACTGTTACACCTGTTCACTGCTCCTTCAACACAATCTTC
AATTCCAACAGCAACTTCACAGGCCTGTTTATACCAAAGCTCCTGCTGGGCATCAGCACGACGACAGGA
ATTTGGGATGAGACACGGCCGCTCTATATAACCATGAAAGCTTTCAAGAAAAATAGGCGCATCAATTAT
TCACAGCCGATCGGTGTTGTTTACTTTTTTCCTAAACAGATCTTGCCACCAGGAAACAACGTTGAGTTC
AATTGGACAGAAGCCTCCAAAATTTACGTTAATACAGAGTCACCCAACGGGCCTGTCCGCAACACAGTG
ACATTCACAAACCAGGCCGTGCTTCGCACCCCCAGCCTGAGCACGGTTGCCAACCTGACTCCTGACATA
AACATGGGAATATTCCTCTCAAGCCTCAGGGTGGCCTTTGACACTGCGCACATGGTTCCTCTGCACTTT
AGCCTGAAGCCAGGAGAGTCTACTAGGATGGAATTCATGCCCCCTGGGACCCCTCAGAATCTGACCATT
CTCGAGGGCGATGTTGGTATCCACTTCATACCCTGTCACAACCACTCACATCGGTCCTCTCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC101404 representing NP_042947
Blue=ORF Red=Cloning site Green=Tag(s)

MQPATLQWSSYVLQLRVTTTAILKPGELRLFKCGLGITPPSSAVVCICRDESSFTSSPFTYIDPKDYGN
IPFAVQNISDLDLDLSRLPIVLNIFALPHANVNISNLPVQRIEAHDRHIIPHGQCDAKFVIYGPLTRIK
IQVADIRWIEQTPEEPTRYSFKAEIWVNLQNTPLDQIFKTGNIEFISHKNVYISRILLCGNLLILKANY
ENDYLLDDNFYPEQLAIQISLTPQTADITLSQNQETLLKCNTHSITVCATKNIIPNTVTPVHCSFNTIF
NSNSNFTGLFIPKLLLGISTTTGIWDETRPLYITMKAFKKNRRINYSQPIGVVYFFPKQILPPGNNVEF
NWTEASKIYVNTESPNGPVRNTVTFTNQAVLRTPSLSTVANLTPDINMGIFLSSLRVAFDTAHMVPLHF
SLKPGESTRMEFMPPGTPQNLTILEGDVGIHFIPCHNHSHRSSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NC_001664
ORF Size 1374 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NP_042947
RefSeq ORF 1374 bp
Locus ID 1487936
MW 51.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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