U30 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101378

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 6], codon optimized for human cell expression, NP_042923



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CNY 14,060.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101378 represents NCBI reference of NP_042923 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCACGAGCCCTGGATCTGGAGTTCATCCTGAGTTCACTGAAAAATTCAACCTCACCTGAGTCCC
TTCTGGCAGCCACTGCTAAAATCGAACTCTTGTCCTTGGCCGCCGATAGCGTAACTCACAATAGGGTTAT
AGCCTTTATTAACCGACTGACACCTAAGAAATACCACTTTGATTTGATCAGACAATATGCTGTATTCTAT
TTCCTGAATTCCACCACCCTTACCAGCGAGAATAAGCTGCTGAGTGCCGAACTCCTGTATGAGGAGCTGA
GCCAAATTCCTTCCAGCAGCTCCTCAGGCACCGAGCTGGAGAACCTGAATAACGCTGAAGTGTTGTACGT
TTTTGATAGAATACTCAATAGCATCAAGATGCTTAAAAACGAGCTCTCAAGCCCCATAGGCAAACTTAGA
GCTCAGCCCGCTGCTAACGACCCCGGCACCGATAAGACTATTAAGTACTCCAAGATTCAGTCCATAATCC
AGAAGATCAACAGTTTTACCCACGAGAATAGCCTCCTGGCTAACTGTAGAGCCGTGGTAGAGTTGATTGA
TGACTTGTATAGAAAGCTTTATAGCTGGTTTCTGCATGTGCTGACATTTGAGGATATTCAGTTCCCAGGT
GACACCTTCCTGGATAGGCTGCTCAAGATGGATTACTGTTTTACATATTACCCGTCCTCAAATAGACATC
TGATCGACCTGTTTGAGAAGACGTTGGATAACCAGACCTTTACCAATTTGGATAAATTCTTTGATACCTC
TGGAAACTCCCCAGAGTTGCTGTATCAGAAGACATTCAGTCTCAAGATATTTTCAAAAAACCTGACCGCC
CAGGACAACGGATTGTACATCTATCCCCTGCTTAAAACTGACTTGTCAATCCTGGACTTTCTGGGCACAG
AGAATATCCTTTTTCATAGGGGACTGATTTACCACATCTTGCACCAGAAGACTATCCCACAAGAGCGGGA
GAACGACTTGAATAAGATTAACCAATTTTTTGCAACAGTCATCCAGCAGGTCATTGAAACCAAAAGTTCT
TGTCTGCCCGCCAGCCTGTCCCAGCTCCTCGACACTATCTTTCACTTTAATCGCATCGGACTTAATATGG
AGACCTGTAGGACTTACATCGAAATACTGTCCAACCACATGGCCACTCCTGATACCCAACCCATCATAAA
CACATTTACTATCAATCTGACCCACATAGTGTTCACCGCACACGTCTTCTTTATTTGCATGGAGAATTTC
AGCCCCACCTTCATCTTCTATAATAGAAAGAAATTGATCCTCGAACAGCAAAGAGCTATTCTCATCATCG
AAAGGAACGATTACAGCACACTTTGGAAACAAATCTCTGATCATATCGAGTGCCTCTTTAACGTAAGCCT
TTCAGAGAGCTTCTTCAAAGAGTACACCAAGGGTGGGAATGAGGATCAGAAACAGTTTCTCTATAAGAAT
CTGTTCGAGAAATGGGGAAACGTCTTCTTCCCATTCACTTATAGCGTGTCCACTTCAAACAACTCTACTG
CCCACCACATCACAACTCTTGAACTCCGCGATATCTGTAAAGAGGTGTATCAGTCTGATTCCCCTGATGC
CTACGAGTCACTTCTTCCCTATAGTACCCACCCGAGCTTTAAGACACTGTTCGTCAAGATTTATGTGATC
CCTATGGTTTCACACATCACCAACCTCACGTTTGACAAGTTGCAGTCCGACTGTCGGTTGCTGACTCTGA
TACATGCCTGCAAGCTGCTGCTGCCTTCTCAGCACCTCTTGCTGCACTATATGGCTTGGCTTTACGCATT
TTCAATAAATGTGGACCATATCGATCTCGGCACATTCACAGTGATCAAGTCCGTGATCTTCAAAATTGCA
GATCACATCAATGTGATGACTCATACTATTTACTCTCCAGAGACAAATCTGCTCGTGAGCATCCTGCTGA
ACGCTTATACGAACTACCTTCAGAAGTACGTGAACCCCTGGATCAAGCAGACCATCACAGCGAACTTTTC
ACTCATTCAAACCTATATTACATTCACCAAACAGTGCGCTTCCATCCTGGCAACAAAGTGCAACATTAAT
CTGGACAATCTGTTCATATACATCACAATTGGGACGGATAAGATTGTAACCACTTCATTCTGCAGTTTTA
TCGCTACGTGCAGGAACCTGGTCCGACAGCACGAGGAGTTTGAGAAGAGTCTGCAGACGATTCAGATTTC
AGAAATTACACTGACAGGCATGCTGAGAAACATCATCACAAGCGTCTCCTCCTCCAAGGAACTGTTGACC
AACGAAGCATTGCAGAAATTCATCGACACGGTGCAGAGAATAAGCCAACACGTGAACGAGACGTATCATT
CTATCTCAGTCAATCTGGAGAAATGTAAGACATCAAACGACATCCTCATTGAAAGTCTCAAAAAAATTAT
CTACATTGTTGACGTCCTGAGCTCCAATGCCATTCTTAATACAAGCTTGGCTAGTCGGTGTCTGGAGGCC
GCAAACCTGGCCGTATCTAATAACTCTTTTACAATTTTGGAAATTAAGAAAGACGCCGTGGCAGTCTTTA
AACCGTTTATTACCCAGCTTTTCGAGAGCATGAAACCCACGACCAGTCTGCACAAAAAACTCATGTCTAC
CCAGAAACTGACCACTGACCACATACCTTTTTTGGACACATTTGACGATAGATATAATCTGGTAAGGCAC
GTCGAAAGGCAGCTTAATTGGTACGCTGCACACGCCGAGGCAGCCCAGCAGGATCTGATCACCCCTCTGA
AGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101378 representing NP_042923
Red=Cloning sites Green=Tags

MAARALDLEFILSSLKNSTSPESLLAATAKIELLSLAADSVTHNRVIAFINRLTPKKYHFDLIRQYAVFY
FLNSTTLTSENKLLSAELLYEELSQIPSSSSSGTELENLNNAEVLYVFDRILNSIKMLKNELSSPIGKLR
AQPAANDPGTDKTIKYSKIQSIIQKINSFTHENSLLANCRAVVELIDDLYRKLYSWFLHVLTFEDIQFPG
DTFLDRLLKMDYCFTYYPSSNRHLIDLFEKTLDNQTFTNLDKFFDTSGNSPELLYQKTFSLKIFSKNLTA
QDNGLYIYPLLKTDLSILDFLGTENILFHRGLIYHILHQKTIPQERENDLNKINQFFATVIQQVIETKSS
CLPASLSQLLDTIFHFNRIGLNMETCRTYIEILSNHMATPDTQPIINTFTINLTHIVFTAHVFFICMENF
SPTFIFYNRKKLILEQQRAILIIERNDYSTLWKQISDHIECLFNVSLSESFFKEYTKGGNEDQKQFLYKN
LFEKWGNVFFPFTYSVSTSNNSTAHHITTLELRDICKEVYQSDSPDAYESLLPYSTHPSFKTLFVKIYVI
PMVSHITNLTFDKLQSDCRLLTLIHACKLLLPSQHLLLHYMAWLYAFSINVDHIDLGTFTVIKSVIFKIA
DHINVMTHTIYSPETNLLVSILLNAYTNYLQKYVNPWIKQTITANFSLIQTYITFTKQCASILATKCNIN
LDNLFIYITIGTDKIVTTSFCSFIATCRNLVRQHEEFEKSLQTIQISEITLTGMLRNIITSVSSSKELLT
NEALQKFIDTVQRISQHVNETYHSISVNLEKCKTSNDILIESLKKIIYIVDVLSSNAILNTSLASRCLEA
ANLAVSNNSFTILEIKKDAVAVFKPFITQLFESMKPTTSLHKKLMSTQKLTTDHIPFLDTFDDRYNLVRH
VERQLNWYAAHAEAAQQDLITPLKF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 2805 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042923
RefSeq ORF 2805 bp
Locus ID 1487908
MW 107.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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