U26 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101374

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope protein UL43 [Human herpesvirus 6], codon optimized for human cell expression, NP_042919



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101374 represents NCBI reference of NP_042919 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCAGGCTCACCGACAGCTTCATCTTGGGATTGGCTAAGGGAGCCGTGATCCCAGGGCTCTACACCT
TCAGAATGACGGAAGGACGGTCCCCCTTGGGGCAGATTGGCGTGTTGATCACCGTCGCAATCTCCTTCCT
GTTGACTTTCAAGCGGTTTGACCCTCGGTTTTATAAACCGATCGGTGACTTCAAGATCGTCTTTTTGTCC
CTGATGGCTCCTAAACTGCCAAGCCTGCTGAGTGCGGTTGTGATGATTTGTTTGATTTTTAGCGAAATGA
GACTGCGGATGATCCTCAGCAGGTGCGTGATGATTATGCCCTCTTACTCCCCAGCCGTCTTCACCGGTAT
CATGGTCAGCTTGTTCTTCAAGAGCCAGATGTTTGACGATTATTCCGTGTTGATTACCGCAGCCTCTCTC
CTTCCAATTACGGTACGGTACGGTTGGATGATCCGCTCAAGTGGCTTCCTCCTGGGGCTGCAGAAATATA
GGCCCATCCTGAAGAGTACCAGTTTCCGCGAGGTTGATCTTAAGTGTCTGGTGAAGTTCACAGTTGAATT
CTTGCTGCTCTTCACAATGCTCTGGATAGGAAAAATGTTCCTGAGTATGCCGAAATCCAATCACCTGTTT
TTCCTGACCGTGGTTAACAACGTCTTTTTTAAACTCAATGTCTTCAAGGCAGCAGCATGCGCTGTGGTCG
CAATCCTGAGCGGCCTGATGATGAATGTGTGCCTGTACCGCATTATCTTTGAAGCCTTCGTTGGTTTGGG
ATTCTCCAGCATCATGTTGACCCTGTCAAGTGACCTGAAAGATCGCTCCTTCTACGCTGGCGATCTCCTG
AATGGATTCTTCTGCCTTGTGGTTTGTTGTATGTATTTCGGGGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101374 representing NP_042919
Red=Cloning sites Green=Tags

MRRLTDSFILGLAKGAVIPGLYTFRMTEGRSPLGQIGVLITVAISFLLTFKRFDPRFYKPIGDFKIVFLS
LMAPKLPSLLSAVVMICLIFSEMRLRMILSRCVMIMPSYSPAVFTGIMVSLFFKSQMFDDYSVLITAASL
LPITVRYGWMIRSSGFLLGLQKYRPILKSTSFREVDLKCLVKFTVEFLLLFTMLWIGKMFLSMPKSNHLF
FLTVVNNVFFKLNVFKAAACAVVAILSGLMMNVCLYRIIFEAFVGLGFSSIMLTLSSDLKDRSFYAGDLL
NGFFCLVVCCMYFGV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 885 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042919
RefSeq ORF 885 bp
Locus ID 1487902
MW 33.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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