U90 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101433

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042983



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CNY 14,160.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101433 represents NCBI reference of NP_042983 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCGCCAAGCCAAGTGGAAATAACATGGGGAGTAATGACGAACGCATGCAGGATTACCGACCCG
ATCCGATGATGGAAGAGTCCATCAAAGAGATACTGGAGGAAAGCTTGATGTGTGATACCAGTTTTGACGA
CTTGATTATACCTGGGCTGGAGAGCTTCGGCCTCATCATTCCTGAGTCATCCAATAACATAGAAAGTAAC
AATGTGGAGGAAGGGAGTGATGGAGAGTTGAAAACTCTGGCCGCCCAGTCTGCTGGCAATTGTATACAGT
CCATTGGTGCCTCCGTCAAAGCGGCGATGAAACAAGAGCAGTCTGACATGGAGGACAAACTTATCAAGTG
TGCCGGCTTGTTGACTCAGCAACAGAGTATGTTCATAGGTCTGGGGCTCGAACAACTGAGCCAGCTGATC
AACATCAATCTGCTCAGCTCTGCCAGTACCAAATACGTTGAAAGCTATAGCAAGATGCTGCATGGAAAAG
AGCTGGATTTCTTCAATTGGTGTGAGCCTCGCTTTATAGTCTTTGCTTGTGATAAATTCGACGGGTTGGT
CAAGAAAGTGGCCTCCGAGTCTCGAAACCTCCTCCTCGACCTGAGGGCAAACATGAACAACGATATTATC
AAGGCCGTGAAAGACATCTTCAGCAAAGCTACCGTCACCTTGGACTGTCAAAAACTTAACCAAGGAGCTA
CCATGTTGATGATGATGGCACATAATAAGGAAATGAGTAATCCCGATATCAGTTCCAAAGACTTTTGCGA
AAAAATTAATACGCTGAAGCAGACACTCCTGGAGGGAAAGAATGAGATTGTGGAGACCAACGCTAAGAAC
ATGCAGATCCTCCAGACCTTCGCCATTAAACAAATGAATCAAATATTCATGGATGGATGTGATAAGGCCT
TCTTGAAGTTGAACGTCAACTGTAAGAATTTGATCACCGCGGCCAAAAATTTGGCTAACACTATCCTCCA
GTCAATTGTGATCTGCAGTAACGAGTTTTCTTGGCAACATCTCAAACTCCTGCGCCGGGGTTTTAAGGTT
ACAATGCTGAACATGATTACCCAGGCCTGTGAATGTCTGGAATCCGATTACGACGATACCGGTCTGATAA
AACCTTTGACTCCTCTGCAGATCATGGACGGATACATCAACATGAACAAAAACAGGCAAAGTTCCATCTG
TGACGGAAATACTGACCCTAGCGATTCCATGATCCTTGACCTGGCTGACTTCGACGATCACGGACGCTAC
AGTGAAGAGAGCAGCATTGAGTCAATCCATGAAGACGATGACAATAAAATGTACCCCTGCACCCCAAGCC
CCGAGGTGCCCGGCAAATCTAAGTACGTAGGCACATTCACTGAGAACAGCCGGCAATCCGGAGATGAACA
GACCAATCCAAACTGCGTCGGAACGGCCTCCGTGACAGACCTTGGAGGGCCTGACAATCTGAACAGTATA
TCCGGCCTGCAGTCCTGTAAAAACATGCTCCTGGAGCGGCTGTTGGATACACAGTGTGATTCTGTTGTGG
AAGGGACAGAACAGGATGGATCTTATGGTAACACTCTGATTAGCGAGATGATGATGTTCGGGTATGAGAC
TGATCATAGTGCTCCCTACGAATCCGAGTCTGACAATAATGATGAAATCGACTACATCGCCAACTCAGAT
TCTGCCGCAAGAACCAATAATATCCATATGAACTCTACCGACGAAAACACTCCATTCTCAAACTCAATCT
GCTCCCCGCCCGAAGTAACGCCATCCAAGAAAAACGTCAAACCAAAGTCTATGACTCCCGGTTCCAAGCC
CAAGAAGAGGGTGGCTAAAAGAAAACATGTCTCTTCAAAAAGCCCTAAAAATAAAAAAATAAAAACAGAT
CAATTGCCAAAGGCGGCAGACGTGATTGTGATCAGCAGTGAGTCCGAAGATGAAGAGGACGGCGATAACA
TTATCGGAAACTCTATCCTGATCAAAGCTATTAAGTCCGAGAGCGACAGTGAGAGTTCTTCTGAGAGTAA
TGACTGTACAAGCGAGCACAAGCAGCTGCACTTGAGTGATTACGATGAGGTAACAAATAATGGCCACTGT
CCCAGCTACGGGTTTCCTACTCCGGTCTTCACCATACCAATCCGAAGCATGCAGGGGACAGGGGGGATTA
AATCTAAATTCATTCCCAAAAAGAATTGGATCTGGTATATGAAGAAGACACATCAAGTGGATAATTGTCC
GATCCATAATTCTGAGAACGTTGATGCTAAAGATGACAGCGATGGCACTGAAGCAAAACACTGTTTTATG
AACCACTTCGTCCCTATTAAAACTGACGATGAAGACTACGATAAGAATAATGTAAGTTACATCTACAACA
AAATACAGAACAGCAAAATTGATTCAGGGGACATTATCCCTACCAAAAAGCTCATCATTGACATGGTTAT
GGACAATTTTATGGATCTGAACGACATTATTAAACAAGGGATCACAAAACACTGTCAGGACCTTTGTAAT
AAATATAACGTTGTCACACCAACCACATGCGAGGATGACCTGAACATGACTAACTCACAAACATTCGCCA
CAACAGCAACCCAGGTTTTCGATCCTCCCGTGACAGGAAATAACTCTAGTATTCTGAATATTATTAATGA
CACAACCTCTCAAAACGACGAGAACAGGTGCACAGAAGGAACCTCAAATTCCAACGAGAAATGTACCAAC
ATCTCAGATTGTAATAGCGATGGCACCGAGGCCTTCAAACTTGACGGATACCCCAGCGACTATGATCCTT
TTGTCGAGAATGCACAGATCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101433 representing NP_042983
Red=Cloning sites Green=Tags

MEPAKPSGNNMGSNDERMQDYRPDPMMEESIKEILEESLMCDTSFDDLIIPGLESFGLIIPESSNNIESN
NVEEGSDGELKTLAAQSAGNCIQSIGASVKAAMKQEQSDMEDKLIKCAGLLTQQQSMFIGLGLEQLSQLI
NINLLSSASTKYVESYSKMLHGKELDFFNWCEPRFIVFACDKFDGLVKKVASESRNLLLDLRANMNNDII
KAVKDIFSKATVTLDCQKLNQGATMLMMMAHNKEMSNPDISSKDFCEKINTLKQTLLEGKNEIVETNAKN
MQILQTFAIKQMNQIFMDGCDKAFLKLNVNCKNLITAAKNLANTILQSIVICSNEFSWQHLKLLRRGFKV
TMLNMITQACECLESDYDDTGLIKPLTPLQIMDGYINMNKNRQSSICDGNTDPSDSMILDLADFDDHGRY
SEESSIESIHEDDDNKMYPCTPSPEVPGKSKYVGTFTENSRQSGDEQTNPNCVGTASVTDLGGPDNLNSI
SGLQSCKNMLLERLLDTQCDSVVEGTEQDGSYGNTLISEMMMFGYETDHSAPYESESDNNDEIDYIANSD
SAARTNNIHMNSTDENTPFSNSICSPPEVTPSKKNVKPKSMTPGSKPKKRVAKRKHVSSKSPKNKKIKTD
QLPKAADVIVISSESEDEEDGDNIIGNSILIKAIKSESDSESSSESNDCTSEHKQLHLSDYDEVTNNGHC
PSYGFPTPVFTIPIRSMQGTGGIKSKFIPKKNWIWYMKKTHQVDNCPIHNSENVDAKDDSDGTEAKHCFM
NHFVPIKTDDEDYDKNNVSYIYNKIQNSKIDSGDIIPTKKLIIDMVMDNFMDLNDIIKQGITKHCQDLCN
KYNVVTPTTCEDDLNMTNSQTFATTATQVFDPPVTGNNSSILNIINDTTSQNDENRCTEGTSNSNEKCTN
ISDCNSDGTEAFKLDGYPSDYDPFVENAQIY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 2823 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042983
RefSeq ORF 2823 bp
Locus ID 1487968
MW 104.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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