U77 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101425

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase helicase subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042970



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CNY 12,540.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101425 represents NCBI reference of NP_042970 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATTTCCAGCCTGTTCGGAGGACGGTATGACAATAAGTTTCTCCTCAATATGAGTTCCGCGCCTA
AGATCGAGCTGATTGTTGATAAGGTTGCATCATTGTCCGAACGGAGGCTGGAGGGGAGACTGCCTGAGGA
CTGGTTTAGACACATTATGGACCCAGAAACCGAGTTTAACTCCGAGTTTGCTGATGCCCTGTGCATCGGC
ATAGACGAATTCGCCCAGCCCCTGCCCTTCCTCCCCTTCAAAGCCCTGCTGGTGACAGGCACAGCCGGTG
CAGGTAAGACAAATTCTATTCAAACCCTGGCCGCAAACCTCGACTGTATCGTCACTGCAACAACTAGTAT
AGCAGCCCAGAATCTGAGCGTAGTGCTGAATCGGTCCAAGTCTGCCCAGGTCAAAACTATTTTCAAAACC
TTTGGCTTCAATTCTAGTCACGTTTCCATGTCAGAGAGACAGTCTTACATTGCTAATGATGAGCGATCCA
TCCAAATCCAACAGAAGCAGGATCTGTCCATCTACTGGAACGTAATAAGCGACATTGCAGACCGAGCTCT
GGGCGCTGTGGCCTGTAAGACGAAAGAGCTCCCAGACCTTTGCGAGTCCTCCGTCATCGTTATAGATGAA
GCTGGCGTAATTCTCAGGCATATACTTCACACCGTGGTGTTTTTTTACTGGTTCTACAATGCCCTCTACA
AGACACCCCTGTACGAAGACGGGATAGTGCCGTGCATTGTCTGTGTCGGTTCTCCAACCCAGAGCAACGC
CTTGGTGACCTCATTTAATCCTCTTACCCAAAACAAGGATGTCAAGCGGGGAATCGACGTCCTGAGCGCA
CTCATCTGCGACGATGTCCTGTCCAAGTACTGTGAGGTAGACAACAACTGGATTATTTTCGTTAATAATA
AGCGCTGCGCTGACCACGCCTTCGGAGATTTCCTGAAGCACATCGAGTTTGGACTGCCGCTGAAACCAGA
GCTTATTGAATATGTTGATCAATTCGTGAAACCAGCATCCTACATCAGGAACCCAATGAATGAGATTGAA
ACTACCAGGCTCTTTCTCAGTCATAATGAAGTGAAAAACTACTTCAGGTCCCTTCATGAACAGGTGGAGG
TGACTAATCGAAACAATCTGTTCGTTTTCCCCGTGTATTTTCTCATAAAGAACAAGACTTTTGAGGATTA
TAAAAGCGAGATAGGGAATTTCAGCCTGGAAATTGAGCCATGGTTTAAATCTAATATCCATAGACTGAAC
ACATACTCCCAGTTCGCTGACCAGGATTTGAGCAAAACAGTCCAGTTGGAGGAAATAGTCCTTGAGGATG
GCTCTGTCGAGGAAACACTGATCACTTGCCACTTGAAACATATACGCAACAGCTCCATTGGAGTGACTAG
CAAGATCAAAGCCTCAACTGTCGGCTTCTCTGGAACTTACGAAAAGTTTGTGGAACTGCTCCAGTCAGAT
CTCTTTATAGAGAAGACCTCTTGTGACCAAACTATCCACGCTTACTCATTCCTGTCAGGCCTGATGTTTG
GGGGCATGTATAGCTTCTGCTGTTCAAAATTTACAACACCGGAGGTACTGATGGAGATTAAGAATATCAA
GATGCCATCAATTGAATTCCTCGAATCCGAGATGTCTAGGATGTCACCTGATGTGCAGACAGTGGAGACT
GACGAAAGGTACGATTTTGGACTGGTCAATGATGGCCTGAGTGACGTCGACCTGCTTGAAATCGATCCAT
GCGGGGACCCGTTTTTTACCCGGTATTCAAAGCTGCCCCTGACAAACTCACTGTCCTTTGAAGAGATTAG
CCTGCTCTACACCACCTTCAAAGACATTTTCATTAGTCGCTTTGCAATACTGCAAAAGCACACAAAAGGC
AAATTCGGAAAGACGTTGCTGGTGACATACAATAGAAATAACGTGAGTCGGAAGCAGTGTGGCGAGATCT
ATAGCCATCTTAAAAGCTTTTATGGTATGCTGACTTACGCCATTCCCGCTAACAACTACACACTCGAAGG
GTATACCAATGACAACGTAGTCCACCTGGGCACAGATAAGCAGCTGCCACAAATCCTGTACAAGAAAGGG
CTGCCTCGCCTGGTGATCAAAGACGAGATGGGGTTCATCTCTGTGCTGGACAACAATGTGTCCAAGTTTA
TAGACGTCGTGAATGGGCAGTCATTTCACTTGTGCACCACCGTGGATTATGCTACCGTCAGCAAGGTGTC
AATGACGATAACCAAGTCCCAGGGTCTGAGCATCCAAAAGGTCGCCATAGACTTTGGCAGCGACCCCAAG
AATTTGAAGCTGTCATCAATCTACGTGGGTATGTCCAGGGTGACAGACCCGAATAATCTCATCATGAATG
TAAATCCTCTGCGACTCAATTATGAGAATGATAATTTCATAGCCCCACACATTGTGAAAGCCCTTAAAAA
TGAGAACACCATGCTGATCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101425 representing NP_042970
Red=Cloning sites Green=Tags

MSISSLFGGRYDNKFLLNMSSAPKIELIVDKVASLSERRLEGRLPEDWFRHIMDPETEFNSEFADALCIG
IDEFAQPLPFLPFKALLVTGTAGAGKTNSIQTLAANLDCIVTATTSIAAQNLSVVLNRSKSAQVKTIFKT
FGFNSSHVSMSERQSYIANDERSIQIQQKQDLSIYWNVISDIADRALGAVACKTKELPDLCESSVIVIDE
AGVILRHILHTVVFFYWFYNALYKTPLYEDGIVPCIVCVGSPTQSNALVTSFNPLTQNKDVKRGIDVLSA
LICDDVLSKYCEVDNNWIIFVNNKRCADHAFGDFLKHIEFGLPLKPELIEYVDQFVKPASYIRNPMNEIE
TTRLFLSHNEVKNYFRSLHEQVEVTNRNNLFVFPVYFLIKNKTFEDYKSEIGNFSLEIEPWFKSNIHRLN
TYSQFADQDLSKTVQLEEIVLEDGSVEETLITCHLKHIRNSSIGVTSKIKASTVGFSGTYEKFVELLQSD
LFIEKTSCDQTIHAYSFLSGLMFGGMYSFCCSKFTTPEVLMEIKNIKMPSIEFLESEMSRMSPDVQTVET
DERYDFGLVNDGLSDVDLLEIDPCGDPFFTRYSKLPLTNSLSFEEISLLYTTFKDIFISRFAILQKHTKG
KFGKTLLVTYNRNNVSRKQCGEIYSHLKSFYGMLTYAIPANNYTLEGYTNDNVVHLGTDKQLPQILYKKG
LPRLVIKDEMGFISVLDNNVSKFIDVVNGQSFHLCTTVDYATVSKVSMTITKSQGLSIQKVAIDFGSDPK
NLKLSSIYVGMSRVTDPNNLIMNVNPLRLNYENDNFIAPHIVKALKNENTMLIF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001664
ORF Size 2472 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001664.2, NP_042970
RefSeq ORF 2472 bp
Locus ID 1487959
MW 93.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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