US22 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101339

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein US22 [Human herpesvirus 5], codon optimized for human cell expression, YP_081607



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CNY 6,940.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101339 represents NCBI reference of YP_081607 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCTGCTGACTAAGGCCGCAGCCGAAGCCTGGGGCACCTATCTGCGGCAGAGAGATGAGCGGTGCG
AAGACGCCATTCGGTGCGATTACGGCGTGTTCCAATTTAGAAACACCGTGTTTCAGAAGACACTGAGCAT
GCTGCAGGGCCTGTATCTCCGGCAATACGATCCCCCCGCCCTCAGAACCTACGTCCAACGGCATCAGGGG
ACTACCGTGGCACTGCGGAACCCCGCCAACTGGTTCCTCGTCATGCGGGAGCAGGCCGCCATCCCTCAGA
TCTACGCCAGAAGCCTCGCCGCAGATTACCTGTGTTGCGACGACACCCTGGAAGCCGTCGGCGTGCTGGC
CGTCCGGCCACCCGACAGCGACCTGACCCGGAACACAAAGCAGGCCCAGGAGCTCCCATGCGTCCTCATG
CTCAGCCACTACGGGACCGTGTACGTCTACGACTGGGAGACCGACGGGCTGTACGAAGTGGCAAGCGACA
TCAAGGCCTTCAGCAAGAACGGCCTGCTCTGGTGCGAGTACGTGTACCGGCACCCCCAGACACCCTTCGC
CACCACCGAGCCCCGGTATCACGTGCAGAAATTCCTGTGCACCGACCCCACCGATGCTGCCGCCGTGGCC
AAGACCGCCCGGGAGATGAACGGCCTGAACCTGGTGATTCGGACCCCCGGGCGGACCGAGGTCGAGCCCC
TCCTCATGCTCGGCAGCATTGAAGGCCTGCGGGCCTGCCGGCCCTTCGACCACATGCCCGCTGCCGACTT
CCGGGACCTGCTCAACTTCATCCGGCAGCGGCTGTGCTGTGAGTGGTACGTCGTGGGCCTGGTCGGCTAC
TACCTCGCCTACGGCCCCTTTGTCCCCTCCGGCATGGTCCTGCTCGATAAGTTCGGCGTGGTGTACCTGC
ACAAGATCGAGGACTCCGACCTGTATCGGATCGCCGACAACTTCCACATGTTCCTGAAGTGCGGCCTCCT
CAAACTGCGGGGCCTGTGCCGGTTCGACCGGGGGCTGCGGGGCGAATGCCGGCTGGAAGAACTGCCCGTG
TGCCACCACACCCTGAAGCGGGACGTGCTGCGGTGGCATGGCGCCCTGGGCACCATCACCCGGAGCCAAC
TCGAGTCCGCCCTGGACTGGTTCCTGCGGCCCACCCGGGGCACTGACAAGGTGCCCAACAACAGCAGCGC
ATGGGGCCGGACCGATCTGCTGCCCACCGGCGCCCTCCAGGACAACCAGAATTGGGCATTCCCCAGCCAC
AGCGTGGAGACCCTGCCCGCCCTGCAGGGGGGCCTCTGGGAGGACAACGACGAGACCACCCAGACCGTGG
ACGGGCAGCGGTGCTTCCGGATGCCCAAGTTCTTCCCCCCCCCCATGTGCCGGGCCCCCTACAACGAGTG
CGGCGTCGAGCTGCCCGGGGGCGACTCCAGCGACGAAGACGAAAGCGGGCGGCCCCGGCGGATCGCCAAC
CGGATCGGCGATACCCCCGAGACACCCTGCAACTCCGAAAACGAGGATGACACCACCGTCGAGGGCACCA
GCGGGGGACCCGAGGCTATGGACAGCCAGGCCCCCTACCCCAGCGAGGACAGCCCCACAACCGAGAAGGA
AGCTTGGCTGCAACGGGGCCGGCGGGCCAAGGCCATGCACGCTCGGGGACACACACTCAAACAAGTGCCC
ATCCCCGAGCCCGACCAAATGGGCGATGACGGCCCAGACCCCGGCCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101339 representing YP_081607
Red=Cloning sites Green=Tags

MSLLTKAAAEAWGTYLRQRDERCEDAIRCDYGVFQFRNTVFQKTLSMLQGLYLRQYDPPALRTYVQRHQG
TTVALRNPANWFLVMREQAAIPQIYARSLAADYLCCDDTLEAVGVLAVRPPDSDLTRNTKQAQELPCVLM
LSHYGTVYVYDWETDGLYEVASDIKAFSKNGLLWCEYVYRHPQTPFATTEPRYHVQKFLCTDPTDAAAVA
KTAREMNGLNLVIRTPGRTEVEPLLMLGSIEGLRACRPFDHMPAADFRDLLNFIRQRLCCEWYVVGLVGY
YLAYGPFVPSGMVLLDKFGVVYLHKIEDSDLYRIADNFHMFLKCGLLKLRGLCRFDRGLRGECRLEELPV
CHHTLKRDVLRWHGALGTITRSQLESALDWFLRPTRGTDKVPNNSSAWGRTDLLPTGALQDNQNWAFPSH
SVETLPALQGGLWEDNDETTQTVDGQRCFRMPKFFPPPMCRAPYNECGVELPGGDSSDEDESGRPRRIAN
RIGDTPETPCNSENEDDTTVEGTSGGPEAMDSQAPYPSEDSPTTEKEAWLQRGRRAKAMHARGHTLKQVP
IPEPDQMGDDGPDPGP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 1728 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081607
RefSeq ORF 1728 bp
Locus ID 3077463
MW 65.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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