UL36 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101226

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein vICA [Human herpesvirus 5], codon optimized for human cell expression, YP_081495



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CNY 5,800.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101226 represents NCBI reference of YP_081495 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACCTTAGGGACACTCTGATGGCCTATGGATGTATTGCTATTCGAGCTGGGGATTTCAACGGCC
TCAATGATTTCCTGGAACAAGAGTGCGGCACCCGACTTCACGTGGCCTGGCCGGAGCGGTGTTTCATCCA
GCTCCGGTCCCGCTCCGCCCTCGGTCCATTTGTGGGCAAGATGGGCACTGTGTGCTCCCAGGGGGCTTAT
GTTTGTTGTCAAGAATACCTGCATCCGTTCGGATTTGTGGAGGGACCAGGGTTCATGCGATATCAGCTGA
TCGTGCTCATTGGACAGCGAGGTGGCATATACTGTTATGACGATCTTAGAGATTGCATATACGAACTCGC
CCCTACAATGAAAGATTTTTTGCGGCACGGATTCCGGCACTGTGACCACTTTCACACCATGCGAGACTAC
CAGAGGCCCATGGTACAGTACGACGATTACTGGAACGCGGTCATGTTGTACCGGGGGGACGTTGAAAGTC
TGTCCGCAGAAGTGACCAAGAGGGGATACGCCTCTTACTCAATCGATGACCCCTTCGATGAATGTCCCGA
CACCCATTTCGCCTTTTGGACGCACAACACTGAGGTCATGAAGTTCAAGGAGACAAGCTTTTCCGTGGTC
CGCGCAGGTGGCTCCATCCAGACCATGGAGTTGATGATTCGGACTGTCCCACGGATCACGTGTTATCACC
AGCTCCTGGGGGCACTGGGTCATGAGGTCCCTGAACGGAAGGAATTCCTGGTGCGACAATACGTCCTGGT
TGATACATTCGGCGTCGTCTATGGGTACGATCCCGCCATGGACGCAGTGTATAGACTGGCCGAGGATGTT
GTGATGTTTACATGTGTGATGGGAAAAAAGGGCCACCGCAACCATCGATTTTCTGGAAGAAGAGAGGCCA
TTGTCCGCCTCGAAAAGACGCCTACATGCCAGCACCCCAAGAAGACTCCTGATCCAATGATAATGTTTGA
CGAGGATGATGACGATGAGCTGAGCCTCCCCCGAAACGTTATGACCCATGAGGAGGCCGAGTCCCGGCTG
TATGATGCCATTACGGAGAACTTGATGCATTGCGTGAAGTTGGTCACTACCGATAGTCCGCTGGCAACAC
ATCTTTGGCCACAGGAATTGCAAGCTCTGTGTGACTCCCCTGCTCTCTCTCTGTGTACCGATGACGTTGA
GGGCGTCAGACAAAAACTTCGGGCTCGGACAGGAAGTCTGCATCACTTCGAGCTCAGCTATCGGTTTCAC
GACGAGGATCCTGAAACCTACATGGGATTTTTGTGGGATATTCCAAGCTGCGACAGATGCGTGCGCAGGC
GACGGTTTAAAGTCTGCGATGTCGGTCGCCGGCATATCATCCCGGGCGCGGCGAATGGCATGCCTCCCCT
CACACCACCCCACGCTTATATGAATAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101226 representing YP_081495
Red=Cloning sites Green=Tags

MDDLRDTLMAYGCIAIRAGDFNGLNDFLEQECGTRLHVAWPERCFIQLRSRSALGPFVGKMGTVCSQGAY
VCCQEYLHPFGFVEGPGFMRYQLIVLIGQRGGIYCYDDLRDCIYELAPTMKDFLRHGFRHCDHFHTMRDY
QRPMVQYDDYWNAVMLYRGDVESLSAEVTKRGYASYSIDDPFDECPDTHFAFWTHNTEVMKFKETSFSVV
RAGGSIQTMELMIRTVPRITCYHQLLGALGHEVPERKEFLVRQYVLVDTFGVVYGYDPAMDAVYRLAEDV
VMFTCVMGKKGHRNHRFSGRREAIVRLEKTPTCQHPKKTPDPMIMFDEDDDDELSLPRNVMTHEEAESRL
YDAITENLMHCVKLVTTDSPLATHLWPQELQALCDSPALSLCTDDVEGVRQKLRARTGSLHHFELSYRFH
DEDPETYMGFLWDIPSCDRCVRRRRFKVCDVGRRHIIPGAANGMPPLTPPHAYMNN

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081495
RefSeq ORF 1428 bp
Locus ID 3077442
MW 54.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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