UL52 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101242

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging protein UL32 [Human herpesvirus 5], codon optimized for human cell expression, YP_081511



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CNY 10,070.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101242 represents NCBI reference of YP_081511 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCCAGTACACACGTTTCCTCCAATGGCCCCACGACGCCACCTCACGGGCCACATACCACATTCT
TGCCACCCACGAGTCCGGCACCCAGCACATCATCCGTAGCTGCCGCAACACTCTGTTCCCCTCAGAGGCA
GGCTGTGTCCCGCTACTCCGGCTGGTCCACAGAATACACTCAATGGCACTCTGATCTGACCACTGAACTG
CTTTGGCACGCACACCCTCGCCAGGTCCCGATGGATGAAGCGCTTGCTGCCGCGGCTGCAGCCTCCTACC
AGGTGAACCCCCAACATCCAGCAAACAGGTATAGACATTACGAGTTCCAGACCCTTTCCCTTGGCACATC
CGAGGTGGACGAGCTGTTGAATTGCTGTGCTGAAGAGACTACTTGCGGGGGCACCCAATCTACTGTGCTC
ACTAATGCGACCAATACGACGAGCTGCGGCGGTGCGGTGGCTGGGAGTAGTAACGCAGGGCCTGCGGGCG
CAAGTGCAGCCTGCGATCTTGACGCCGAACTTGCCGGCCTCGAAACGTCCGCTGCCGACTTTGAGCAACT
GCGGCGCTTGTGCGCCCCACTGGCTATTGACACACGCTGCAATCTGTGTGCAATTATCTCAATATGTCTG
AAACAGGACTGCGACCAGTCCTGGCTGCTCGAGTATTCTCTCCTTTGTTTCAAGTGCTCTTATGCGCCCC
GCGCCGCACTCAGTACTCTGATTATTATGTCCGAGTTCACCCATCTGCTCCAGCAGCACTTTTCTGACCT
TAGGATCGATGACCTGTTTCGGCACCACGTGCTGACAGTGTTCGATTTCCACCTGCATTTTTTTATAAAC
CGCTGTTTTGAGAAGCAGGTGGGAGATGCTGTCGATAATGAGAATGTCACCCTGAACCACCTGGCTGTCG
TAAGAGCAATGGTCATGGGTGAGGACACGGTGCCGTACAATAAGCCGCGGCGCCATCCCCAGCAGAAGCA
GAAAAACAATCCTTATCACGTCGAGGTACCGCAAGAGCTGATTGACAACTTTCTGGAGCATTCAAGCCCC
AGTAGAGACAGGTTTGTACAGCTCCTGTTCTATATGTGGGCCGGGACCGGGGTCATGAGCACCACCCCGC
TCACTGAGCTGACCCACACAAAGTTCGCTCGGCTGGATGCACTGTCTACCGCATCTGAGAGGGAAGATGC
TCGCATGATGATGGAGGAGGAGGAAGACGAAGAAGGAGGTGAAAAAGGGGGTGATGACCCAGGCAGACAC
AATGGCGGAGGTACGAGCGGCGGCTTTTCAGAGAGTACCCTGAAAAAGAATGTGGGTCCTATTTATCTTT
GTCCCGTGCCTGCATTCTTCACCAAGAACCAAACCTCTACCGTGTGTCTGCTGTGCGAACTGATGGCATG
TTCATATTATGACAATGTGGTATTGAGGGAATTGTACCGCCGCGTGGTGTCATACTGCCAGAATAACGTT
AAAATGGTTGACAGGATTCAACTGGTTCTGGCAGATCTTCTCAGGGAGTGTACCTCCCCCCTTGGTGCCG
CCCACGAAGACGTGGCAAGGTGTGGCCTGGAGGCACCTACATCACCTGGGGGTGACTCAGATTACCACGG
GTTGTCAGGTGTGGACGGCGCTCTGGCCAGACCCGATCCCGTTTTCTGTCATGTCCTCAGGCAGGCCGGA
GTAACCGGCATATACAAGCATTTCTTCTGCGACCCGCAATGCGCCGGGAATATCAGGGTTACCAATGAAG
CAGTACTTTTCGGCCATTTGCACCCGCATCATGTTCAGGAAGTGAAGCTGGCCATTTGTCATGACAACTA
CTATATTAGCCGGCTCCCACGGCGGGTATGGCTGTGTATAACATTGTTCAAGGCCTTTCAAATTACCAAG
AGAACTTATAAAGGCAAGGTGCACCTGGCTGACTTCATGCGCGATTTCACTCAGCTCTTGGAAAATTGCG
ACATAAAACTTGTGGACCCGACATACGTCATCGACAAGTATGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101242 representing YP_081511
Red=Cloning sites Green=Tags

MNPSTHVSSNGPTTPPHGPHTTFLPPTSPAPSTSSVAAATLCSPQRQAVSRYSGWSTEYTQWHSDLTTEL
LWHAHPRQVPMDEALAAAAAASYQVNPQHPANRYRHYEFQTLSLGTSEVDELLNCCAEETTCGGTQSTVL
TNATNTTSCGGAVAGSSNAGPAGASAACDLDAELAGLETSAADFEQLRRLCAPLAIDTRCNLCAIISICL
KQDCDQSWLLEYSLLCFKCSYAPRAALSTLIIMSEFTHLLQQHFSDLRIDDLFRHHVLTVFDFHLHFFIN
RCFEKQVGDAVDNENVTLNHLAVVRAMVMGEDTVPYNKPRRHPQQKQKNNPYHVEVPQELIDNFLEHSSP
SRDRFVQLLFYMWAGTGVMSTTPLTELTHTKFARLDALSTASEREDARMMMEEEEDEEGGEKGGDDPGRH
NGGGTSGGFSESTLKKNVGPIYLCPVPAFFTKNQTSTVCLLCELMACSYYDNVVLRELYRRVVSYCQNNV
KMVDRIQLVLADLLRECTSPLGAAHEDVARCGLEAPTSPGGDSDYHGLSGVDGALARPDPVFCHVLRQAG
VTGIYKHFFCDPQCAGNIRVTNEAVLFGHLHPHHVQEVKLAICHDNYYISRLPRRVWLCITLFKAFQITK
RTYKGKVHLADFMRDFTQLLENCDIKLVDPTYVIDKYV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2004 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081511
RefSeq ORF 2004 bp
Locus ID 3077432
MW 74.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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