UL89 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101269

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging terminase subunit 1 [Human herpesvirus 5], codon optimized for human cell expression, YP_081537



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CNY 10,170.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101269 represents NCBI reference of YP_081537 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGGGGCGACAGCGCCGCCAAGATCCAGGAGAGATATGCAGAGCTGCAGAAACGGAAAAGCCACC
CAACCAGCTGTATCAGCACCGCATTTACCAATGTGGCCGCACTGTGCCGGAAACGGTACCAGATGATGCA
CCCTGAGCTGGGCCTGGCTCATTCCTGTAACGAAGCCTTTCTCCCCCTCATGGCCTTTTGCGGACGGCAC
CGGGATTACAACAGCCCTGAGGAGTCCCAGCGGGAGCTCCTGTTTCACGAGAGACTCAAAAGCGCACTCG
ACGAACTGACTTTCCGGCCCTGCTCCGAGGAGCAGCGGGCTTCCTACCAAAAGCTCGACGCCCTGACCGA
GCTGTATAGAGACCCCCAGTTCCAACAGATCAACAACTTCATGACCGACTTCAAGAAATGGCTGGACGGC
GGCTTCTCCACCGCTGTGGAGGGCGACGCCAAGGCCATTCGGCTGGAACCCTTCCAAAAAAACCTGCTGA
TCCACGTGATTTTCTTTATTGCCGTGACCAAAATCCCTGTGCTGGCCAACCGGGTCCTCCAGTATCTGAT
CCACGCCTTCCAGATCGATTTCCTGAGCCAGACCTCCATCGACATCTTCAAGCAGAAGGCCACCGTGTTC
CTCGTGCCCCGGCGGCACGGGAAAACCTGGTTCATCATCCCCATCATCAGCTTCCTGCTGAAGCACATGA
TCGGCATCAGCATCGGCTACGTGGCCCACCAGAAGCACGTGAGCCAGTTCGTGCTCAAGGAGGTCGAGTT
CCGGTGCCGGCATACCTTCGCCCGGGACTACGTGGTCGAGAACAAGGACAACGTCATCAGCATCGACCAC
CGGGGCGCCAAAAGCACTGCCCTGTTCGCTAGCTGCTACAACACCAACAGCATTCGGGGGCAGAACTTCC
ACCTGCTGCTCGTGGACGAGGCCCACTTCATCAAGAAGGAGGCCTTCAACACCATCCTGGGCTTCCTGGC
CCAGAACACAACCAAGATTATCTTCATCAGCAGCACTAACACCACCTCCGACGCCACCTGCTTCCTGACC
CGGCTCAACAACGCCCCCTTCGACATGCTGAACGTGGTGAGCTACGTGTGCGAGGAGCACCTGCATAGCT
TCACCGAAAAGGGGGACGCCACCGCCTGCCCCTGCTACCGGCTGCACAAACCAACCTTTATCAGCCTCAA
CAGCCAGGTGCGGAAGACCGCTAACATGTTCATGCCCGGCGCCTTCATGGACGAAATCATCGGCGGCACC
AATAAAATCAGCCAGAACACCGTGCTGATCACAGACCAGAGCCGGGAGGAGTTTGACATCCTGCGGTATT
CCACCCTGAACACCAACGCCTATGACTACTTCGGCAAGACCCTCTACGTCTACCTGGACCCCGCATTCAC
CACCAACCGGAAGGCAAGCGGCACCGGAGTGGCCGCCGTCGGCGCCTACCGGCACCAGTTCCTGATCTAC
GGGCTGGAGCACTTCTTCCTGCGGGACCTCTCCGAGTCCAGCGAGGTCGCCATTGCCGAGTGCGCCGCCC
ACATGATCATCAGCGTGCTCAGCCTGCATCCCTATCTCGACGAGCTGCGGATCGCCGTCGAAGGGAACAC
CAACCAGGCCGCCGCCGTGCGGATCGCCTGCCTGATCCGGCAGAGCGTGCAGAGCAGCACCCTCATCCGG
GTGCTCTTCTACCACACCCCCGACCAGAACCACATCGAGCAGCCATTCTACCTGATGGGCCGGGACAAGG
CCCTGGCCGTGGAGCAGTTCATCAGCCGGTTCAACAGCGGGTACATCAAAGCCAGCCAGGAGCTGGTGAG
CTACACCATCAAGCTCAGCCACGACCCCATCGAGTACCTGCTGGAGCAGATCCAGAACCTCCACCGGGTC
ACCCTGGCCGAGGGCACCACCGCCCGGTACAGCGCCAAGCGGCAGAACCGGATCAGCGACGACCTCATCA
TCGCAGTGATCATGGCCACCTACCTGTGCGACGACATCCACGCCATCCGGTTCCGGGTGAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101269 representing YP_081537
Red=Cloning sites Green=Tags

MLRGDSAAKIQERYAELQKRKSHPTSCISTAFTNVAALCRKRYQMMHPELGLAHSCNEAFLPLMAFCGRH
RDYNSPEESQRELLFHERLKSALDELTFRPCSEEQRASYQKLDALTELYRDPQFQQINNFMTDFKKWLDG
GFSTAVEGDAKAIRLEPFQKNLLIHVIFFIAVTKIPVLANRVLQYLIHAFQIDFLSQTSIDIFKQKATVF
LVPRRHGKTWFIIPIISFLLKHMIGISIGYVAHQKHVSQFVLKEVEFRCRHTFARDYVVENKDNVISIDH
RGAKSTALFASCYNTNSIRGQNFHLLLVDEAHFIKKEAFNTILGFLAQNTTKIIFISSTNTTSDATCFLT
RLNNAPFDMLNVVSYVCEEHLHSFTEKGDATACPCYRLHKPTFISLNSQVRKTANMFMPGAFMDEIIGGT
NKISQNTVLITDQSREEFDILRYSTLNTNAYDYFGKTLYVYLDPAFTTNRKASGTGVAAVGAYRHQFLIY
GLEHFFLRDLSESSEVAIAECAAHMIISVLSLHPYLDELRIAVEGNTNQAAAVRIACLIRQSVQSSTLIR
VLFYHTPDQNHIEQPFYLMGRDKALAVEQFISRFNSGYIKASQELVSYTIKLSHDPIEYLLEQIQNLHRV
TLAEGTTARYSAKRQNRISDDLIIAVIMATYLCDDIHAIRFRVS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2022 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081537
RefSeq ORF 2022 bp
Locus ID 3077553
MW 77.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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