UL54 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101244

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 5], codon optimized for human cell expression, YP_081513



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CNY 18,810.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101244 represents NCBI reference of YP_081513 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTTCAACCCATATCTGTCTGGCGGAGTGACCGGAGGCGCCGTAGCCGGTGGACGGCGACAGCGAA
GCCAACCTGGGAGTGCTCAGGGAAGTGGTAAAAGGCCACCACAGAAGCAGTTCCTGCAGATCGTCCCTCG
AGGAGTGATGTTTGATGGCCAGACCGGCCTTATCAAGCACAAAACAGGGCGACTTCCGCTCATGTTCTAT
CGCGAGATAAAGCACCTCCTCAGTCATGATATGGTCTGGCCGTGCCCATGGAGGGAGACCCTGGTGGGCC
GGGTCGTGGGTCCGATCAGATTCCATACTTATGATCAGACAGACGCGGTGCTTTTCTTCGATTCACCTGA
GAACGTGAGCCCTCGCTATCGCCAGCATTTGGTGCCCAGTGGCAATGTTCTGCGGTTTTTCGGCGCCACC
GAGCATGGATACAGTATTTGCGTGAATGTGTTCGGTCAGCGGAGTTACTTTTATTGTGAATACAGTGACA
CAGACAGGCTGAGAGAAGTGATTGCTAGTGTGGGAGAGCTGGTGCCCGAGCCCAGAACACCTTATGCGGT
CTCAGTCACACCGGCGACTAAAACCTCCATTTATGGGTACGGCACGCGCCCTGTCCCAGACCTGCAGTGT
GTAAGTATAAGTAACTGGACCATGGCAAGGAAAATAGGGGAATATCTTCTGGAGCAAGGTTTCCCTGTGT
ATGAAGTGCGCGTGGACCCACTTACGAGGCTGGTGATTGATCGGAGAATTACTACCTTTGGATGGTGCAG
TGTCAACAGGTACGACTGGAGACAACAGGGCCGCGCAAGTACATGCGACATAGAGGTCGACTGTGACGTA
TCTGACCTTGTTGCCGTTCCCGACGACAGCAGTTGGCCTAGATATAGGTGCCTCAGTTTTGACATCGAAT
GCATGTCTGGTGAAGGGGGGTTCCCTTGCGCAGAGAAAAGCGACGATATCGTCATCCAAATCTCTTGCGT
GTGCTACGAAACCGGCGGAAACACAGCAGTGGATCAAGGCATCCCGAATGGAAACGACGGACGGGGCTGT
ACCTCTGAAGGAGTCATTTTCGGGCATTCTGGCCTCCATCTGTTCACTATCGGAACTTGCGGACAGGTTG
GACCAGATGTTGACGTTTACGAGTTTCCCTCCGAGTACGAGTTGCTGCTGGGCTTTATGCTCTTCTTTCA
ACGGTACGCCCCCGCGTTCGTTACGGGCTACAATATCAACAGCTTTGACTTGAAGTACATTCTGACGAGG
CTGGAATATCTCTATAAAGTGGATTCACAGCGCTTCTGTAAACTGCCAACTGCTCAGGGCGGCAGGTTTT
TTCTGCACTCTCCTGCCGTGGGCTTTAAGAGACAATATGCTGCAGCCTTTCCTAGCGCCTCACACAACAA
CCCAGCCAGTACGGCAGCAACCAAGGTCTACATTGCCGGCAGCGTAGTGATTGACATGTATCCAGTATGC
ATGGCCAAGACTAACTCACCCAACTACAAGCTTAATACCATGGCTGAGCTCTACCTGAGGCAGCGGAAAG
ACGACCTGTCATACAAAGATATCCCAAGGTGCTTCGTTGCAAATGCTGAGGGACGGGCTCAAGTTGGCCG
ATATTGCCTCCAGGATGCTGTATTGGTGAGAGATCTCTTTAACACAATCAATTTCCACTATGAAGCTGGG
GCCATTGCTCGACTGGCAAAAATCCCACTCAGACGAGTCATCTTTGATGGCCAACAGATTCGCATCTACA
CAAGCCTGCTGGATGAGTGTGCTTGTAGAGACTTCATCTTGCCAAATCACTACAGCAAGGGTACGACCGT
TCCCGAGACTAACTCTGTGGCCGTGAGTCCTAATGCTGCGATTATTTCCACCGCAGCAGTGCCTGGTGAC
GCTGGCTCTGTGGCCGCCATGTTCCAGATGAGTCCTCCTCTGCAGAGTGCCCCCAGCTCCCAGGACGGCG
TCTCCCCAGGGTCTGGCTCTAACAGTTCATCATCTGTAGGAGTCTTCAGCGTCGGAAGTGGATCCAGCGG
GGGGGTCGGGGTTTCAAATGACAATCACGGGGCTGGCGGGACAGCAGCTGTGTCATATCAGGGCGCCACC
GTTTTTGAGCCAGAGGTAGGTTATTACAATGACCCAGTCGCTGTGTTCGACTTCGCCTCCCTTTATCCTT
CAATCATCATGGCACACAACTTGTGCTATAGCACCCTCCTTGTGCCTGGGGGCGAATATCCCGTCGATCC
GGCTGATGTGTACAGCGTGACCCTGGAGAATGGGGTCACTCATCGCTTCGTCAGAGCCAGTGTTCGGGTG
TCCGTCCTTTCAGAGCTCCTGAATAAATGGGTGTCTCAGCGGCGAGCCGTTCGAGAGTGTATGCGGGAGT
GTCAGGATCCTGTGCGCCGCATGCTTCTGGATAAGGAGCAGATGGCCCTTAAAGTGACCTGCAATGCTTT
TTACGGTTTTACGGGGGTGGTGAACGGGATGATGCCCTGCCTCCCCATTGCTGCTAGTATAACAAGAATT
GGAAGAGATATGCTGGAAAGAACAGCTAGGTTCATCAAGGACAATTTTAGTGAACCCTGTTTCCTTCATA
ACTTCTTTAACCAAGAAGACTACGTGGTGGGCACCAGGGAAGGCGATTCTGAGGAGTCCTCTACCCTCCC
TGAGGGCCTGGAAACCTCAAGTGGGGGCCTGAATGAAAGGAGGGTCGAGGCACGGGTTATCTACGGGGAC
ACCGACTCTGTGTTTGTCAGATTTCGAGGACTGACCCCTCAAGCCCTTGTTGCTAGGGGGCCATCCCTCG
CACACTACGTAACCGCTTGCCTGTTCGTGGAGCCCGTGAAGTTGGAGTTCGAGAAGGTATTCGTCTCACT
CATGATGATCTGCAAAAAACGATATATTGGCAAAGTGGAAGGTGCCAGCGGTTTGTCAATGAAGGGTGTC
GACCTGGTTAGGAAAACCGCTTGTGAATTCGTGAAAGGCGTCACCCGGGACGTGCTTAGCCTTTTGTTTG
AAGACAGGGAGGTCTCAGAAGCTGCTGTCAGGCTTTCAAGATTGTCCCTCGACGAAGTGAAAAAGTATGG
GGTGCCACGGGGCTTTTGGAGAATCCTGCGACGGTTGGTGCAGGCAAGGGACGATCTTTACCTGCATCGG
GTGAGGGTTGAGGACCTGGTGCTTAGTAGCGTACTGAGCAAGGACATCTCTCTTTATCGACAATCAAACT
TGCCACACATCGCGGTGATCAAGCGGCTGGCAGCGAGGAGCGAAGAATTGCCCAGCGTCGGCGATAGAGT
GTTCTATGTTCTTACGGCTCCAGGCGTGCGGGCAGCACCACAAGGGTCATCCGATAATGGGGATTCAGTG
ACCACCGGCGTCGTTTCAAGATCCGACGCCATAGACGGGACCGATGATGATGCCGATGGCGGGGGCGTTG
AAGAATCCAACCGGCGCGGTGGAGAGCCTGCCAAGAAACGAGCTAGGAAGCCCCCATCCGCCGTGTGCAA
CTACGAGGTTGCCGAAGACCCTAGCTATGTGAGAGAACATGGCGTGCCCATACACGCCGATAAGTATTTC
GAGCAGGTGCTGAAGGCCGTGACTAACGTTCTCAGTCCTGTATTCCCTGGGGGGGAGACCGCCAGGAAGG
ACAAATTCCTCCACATGGTGCTCCCCAGACGGCTTCATCTCGAGCCTGCATTCCTGCCTTACAGCGTGAA
GGCCCACGAATGCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101244 representing YP_081513
Red=Cloning sites Green=Tags

MFFNPYLSGGVTGGAVAGGRRQRSQPGSAQGSGKRPPQKQFLQIVPRGVMFDGQTGLIKHKTGRLPLMFY
REIKHLLSHDMVWPCPWRETLVGRVVGPIRFHTYDQTDAVLFFDSPENVSPRYRQHLVPSGNVLRFFGAT
EHGYSICVNVFGQRSYFYCEYSDTDRLREVIASVGELVPEPRTPYAVSVTPATKTSIYGYGTRPVPDLQC
VSISNWTMARKIGEYLLEQGFPVYEVRVDPLTRLVIDRRITTFGWCSVNRYDWRQQGRASTCDIEVDCDV
SDLVAVPDDSSWPRYRCLSFDIECMSGEGGFPCAEKSDDIVIQISCVCYETGGNTAVDQGIPNGNDGRGC
TSEGVIFGHSGLHLFTIGTCGQVGPDVDVYEFPSEYELLLGFMLFFQRYAPAFVTGYNINSFDLKYILTR
LEYLYKVDSQRFCKLPTAQGGRFFLHSPAVGFKRQYAAAFPSASHNNPASTAATKVYIAGSVVIDMYPVC
MAKTNSPNYKLNTMAELYLRQRKDDLSYKDIPRCFVANAEGRAQVGRYCLQDAVLVRDLFNTINFHYEAG
AIARLAKIPLRRVIFDGQQIRIYTSLLDECACRDFILPNHYSKGTTVPETNSVAVSPNAAIISTAAVPGD
AGSVAAMFQMSPPLQSAPSSQDGVSPGSGSNSSSSVGVFSVGSGSSGGVGVSNDNHGAGGTAAVSYQGAT
VFEPEVGYYNDPVAVFDFASLYPSIIMAHNLCYSTLLVPGGEYPVDPADVYSVTLENGVTHRFVRASVRV
SVLSELLNKWVSQRRAVRECMRECQDPVRRMLLDKEQMALKVTCNAFYGFTGVVNGMMPCLPIAASITRI
GRDMLERTARFIKDNFSEPCFLHNFFNQEDYVVGTREGDSEESSTLPEGLETSSGGLNERRVEARVIYGD
TDSVFVRFRGLTPQALVARGPSLAHYVTACLFVEPVKLEFEKVFVSLMMICKKRYIGKVEGASGLSMKGV
DLVRKTACEFVKGVTRDVLSLLFEDREVSEAAVRLSRLSLDEVKKYGVPRGFWRILRRLVQARDDLYLHR
VRVEDLVLSSVLSKDISLYRQSNLPHIAVIKRLAARSEELPSVGDRVFYVLTAPGVRAAPQGSSDNGDSV
TTGVVSRSDAIDGTDDDADGGGVEESNRRGGEPAKKRARKPPSAVCNYEVAEDPSYVREHGVPIHADKYF
EQVLKAVTNVLSPVFPGGETARKDKFLHMVLPRRLHLEPAFLPYSVKAHECC

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 3726 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081513
RefSeq ORF 3726 bp
Locus ID 3077501
MW 137.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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