UL104 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101282

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for capsid portal protein [Human herpesvirus 5], codon optimized for human cell expression, YP_081550



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CNY 10,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101282 represents NCBI reference of YP_081550 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAAACCACTGGAACGAAAAGTCCAGCGGCGCTAAGCGGAGCCGGGAGAGAGACCTCACCCTGT
CCACCATCCGGTCCATCCTGGCTGCCGATGAGCGGCTGAGAATCAAGGCTTCCAGCTACCTCGGCGTCGG
CCGGGGCGTGGACGATGAAGCAGTCATCGACATTTTTCCCACCGGCCAAACCATGAGCTTCCTCCGGCTC
CTGCACGGGTTTCTGGGCACCTGCCGGGGCCAAAGCATGCACCAAGTCCTGAGAGACCCCTGCGTGCTGC
GGAAGCAGCTGCTCTACGGGGTGTGCAAGACCCTCTTCGACACCATCACCGTGCGGAGAGTGGCAGAGGA
GTGGAAGCTGCACGCCGCCCTGTTTCCCTACCGGGCACTGGACGAGGAGGACCTCGAGCAATACCTCCTG
GTGTGGTCCGCATCCCTGCGGCAGAGCGTGCAGACTGGAGTCCTGGGAGCCCTGCGGGATATTCTCTATC
AGTACGCCGACAACGATGATTACGGCCTGTACGTCGATTGGTGCGTGACCGTCGGACTGGTGCCCCTCCT
CGACGTGAAGACCAAGCCTTCCGAGGCTGCTGAACGGGCACAGTTTGTCCGGGCCGCCGTGCAGCGGGCA
ACCGAGACCCATCCCCTGGCACAGGACCTCCTCCAAGCCAATCTCGCCCTGCTGCTGCAGGTCGCCGAGC
GGCTGGGCGCCGTCCGGATCGCCAACGCCCCCGAGGTCCGGGTGTTCAAGAAAGTGCGGAGCGAGCGGCT
GGAGGCACAGCTCCGGGGCAAGCACATCCGGCTGTACGTGGCAGCCGAGCCCCTGGCCTACGAACGGGAT
AAGCTGCTCTTCACCACCCCCGTGGCCCACCTGCACGAGGAGATCCTGCGGTACGACGGGCTGTGCCGGC
ATCAGAAGATCTGCCAGCTGCTGAACACCTTCCCCGTCAAGGTGGTCACCGCCTCCCGGCATGAGCTCAA
CTGCAAGAAGCTCGTGGAGATGATGGAGCAGCACGATCGGGGGAGCGACGCCAAGAAGTCCATCATGAAG
TTCCTGCTCAACGTGAGCGACTCCAAGAGCCGGATCGGGATCGAGGACAGCGTCGAGAGCTTCCTCCAAG
ACCTCACCCCCAGCCTGGTCGATCAGAACAGACTGCTCCCAGCACGGGGGCCCGGCGGCCCCGGGGTGGT
CGGACCTGGCGGAGCTGTCGTCGGGGGGCCCGCAGGCCACGTCGGCCTGCTGCCCCCACCACCCGGCCCC
GCCGCCCCCGAGCGGGACATCCGGGATCTGTTCAAGAAGCAGGTGATCAAGTGCCTGGAGGAGCAGATCC
AGAGCCAGGTGGACGAGATCCAGGACCTGCGGACCCTGAACCAGACCTGGGAAAACCGGGTGCGGGAGCT
CCGGGACCTGCTCACCCGGTATGCAAGCCGGCGGGAGGACTCCATGAGCCTCGGCGCCCGGGACGCAGAG
CTGTACCACCTCCCCGTGCTCGAGGCCGTGCGGAAGGCCCGGGACGCCGCACCCTTCCGGCCCCTCGCCG
TGGAGGACAACCGGCTCGTCGCCAACAGCTTCTTCAGCCAGTTCGTGCCCGGCACCGAAAGCCTGGAGCG
GTTCCTGACCCAGCTGTGGGAAAACGAGTACTTCAGAACCTTCCGGCTGCGGAGACTGGTCACCCACCAG
GGCGCCGAGGAGGCCATCGTGTACTCCAACTACACCGTCGAGCGGGTGACCCTGCCCTACCTGTGCCACA
TCCTGGCCCTCGGCACCCTGGATCCAGTGCCCGAGGCCTACCTCCAACTGTCCTTCGGCGAGATCGTCGC
CGCCGCCTACGACGACAGCAAGTTCTGCCGGTACGTCGAGCTGATCTGCAGCCGGGAAAAGGCACGGCGG
CGGCAGATGAGCCGGGAGGCCGCCGGCGGGGTGCCCGAGAGAGGCACCGCCAGCAGCGGCGGGCCCGGAA
CCCTGGAAAGATCCGCACCCCGGCGGCTGATCACTGCCGACGAAGAACGGCGGGGGCCCGAACGGGTGGG
GCGGTTCCGGAACGGCGGGCCCGACGACCCACGGCGGGCAGGGGGGCCATACGGGTTCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101282 representing YP_081550
Red=Cloning sites Green=Tags

MERNHWNEKSSGAKRSRERDLTLSTIRSILAADERLRIKASSYLGVGRGVDDEAVIDIFPTGQTMSFLRL
LHGFLGTCRGQSMHQVLRDPCVLRKQLLYGVCKTLFDTITVRRVAEEWKLHAALFPYRALDEEDLEQYLL
VWSASLRQSVQTGVLGALRDILYQYADNDDYGLYVDWCVTVGLVPLLDVKTKPSEAAERAQFVRAAVQRA
TETHPLAQDLLQANLALLLQVAERLGAVRIANAPEVRVFKKVRSERLEAQLRGKHIRLYVAAEPLAYERD
KLLFTTPVAHLHEEILRYDGLCRHQKICQLLNTFPVKVVTASRHELNCKKLVEMMEQHDRGSDAKKSIMK
FLLNVSDSKSRIGIEDSVESFLQDLTPSLVDQNRLLPARGPGGPGVVGPGGAVVGGPAGHVGLLPPPPGP
AAPERDIRDLFKKQVIKCLEEQIQSQVDEIQDLRTLNQTWENRVRELRDLLTRYASRREDSMSLGARDAE
LYHLPVLEAVRKARDAAPFRPLAVEDNRLVANSFFSQFVPGTESLERFLTQLWENEYFRTFRLRRLVTHQ
GAEEAIVYSNYTVERVTLPYLCHILALGTLDPVPEAYLQLSFGEIVAAAYDDSKFCRYVELICSREKARR
RQMSREAAGGVPERGTASSGGPGTLERSAPRRLITADEERRGPERVGRFRNGGPDDPRRAGGPYGFH

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2091 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081550
RefSeq ORF 2091 bp
Locus ID 3077547
MW 78.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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