UL70 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101249

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 5], codon optimized for human cell expression, YP_081518



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CNY 14,250.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101249 represents NCBI reference of YP_081518 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGGTTCTGTTCGCAACAGAATATGACTCAGCCCATATCGTTGCCAATGTGCTTAGTCAAACAC
CAACCGACCATTGCGTCTTCCCCCTGCTGGTAAAACATCAGGTGAGCAGACGAGTTTACTTTTGTCTGCA
GACACAGAAGTGTTCTGATAGCCGGCGAGTGGCTCCTGTGTTTGCAGTGAATAATGAAACCCTTCAGCTG
TCCCGATACCTGGCCGCGCGCCAACCTATCCCACTGTCCGCTCTGATCGCATCCCTTGACGAGGCGGAAA
CCCGCCCATTGTACAGACATCTGTTCCGGACTCCCGTGCTCTCACCTGAGCACGGTGGGGAGGTGAGGGA
ATTCAAGCACCTGGTCTACTTTCACCACGCCGCAGTGTTGAGACACCTGAATCAGGTCTTCCTGTGCCCT
ACCTCACCTAGCTGGTTTATATCTGTCTTTGGGCACACTGAGGGTCAGGTTCTGCTTACCATGGCCTACT
ATCTTTTCGAGGGCCAGTATAGCACGATTTCAACTGTGGAAGAGTACGTACGCTCCTTCTGTACAAGAGA
CCTCGGGACAATCATCCCAACTCACGCATCCATGGGCGAGTTTGCAAGACTCCTTCTGGGCAGCCCGTTT
AGGCAGAGGGTTAGCGCCTTTGTGGCCTACGCCGTCGCACGAAATAGGCGGGACTACACGGAGTTGGAGC
AGGTTGATACCCAAATCAACGCATTCCGCGAGAGAGCCCGCCTTCCCGACACAGTGTGCGTTCACTACGT
GTATCTGGCATACAGAACCGCGCTCGCCAGGGCACGACTGCTGGAGTACCGGCGCGTGGTTGCTTACGAC
GCCGATGCAGCACCTGAGGCCCAGTGCACCAGGGAACCAGGGTTCCTTGGGAGAAGGTTGTCAACCGAGC
TGCTGGATGTCATGCAAAAGTATTTTTCTCTGGACAATTTCTTGCACGATTATGTAGAGACTCATCTGCT
GCGCCTGGACGAGTCCCCTCATAGCGCTACCAGCCCACACGGTCTGGGGCTTGCCGGATACGGGGGCCGA
ATTGACGGCACCCACCTTGCTGGATTTTTTGGTACATCTACCCAGCTCGCCCGCCAGCTCGAAAGGATCA
ACACCCTGTCCGAATCCGTGTTCTCACCCCTCGAGCGGAGTCTGTCAGGGCTGCTTAGGTTGTGTGCATC
TCTGAGGACCGCCCAGACATACACAACTGGCACCCTGACGAGATACTCCCAGAGAAGATACCTGCTGCCC
GAACCTGCCCTCGCTCCACTCCTGGAACGCCCCCTGCCCGTGTACCGCGTACACCTGCCAAACGATCAAC
ATGTGTTCTGCGCCGTGGCCTCAGAGACCTGGCACCGAAGCCTGTTTCCAAGAGACCTCCTGCGCCATGT
TCCAGATAGCAGATTCAGCGATGAGGCCCTGACCGAGACAGTCTGGTTGCATGATGATGACGTTGCTTCA
ACTAGTCCAGAGACTCAGTTTTACTACACCAGACACGAGGTGTTCAACGAACGATTGCCGGTATTTAATT
TTGTCGCCGATTTTGACCTTAGACTTAGAGACGGTGTGTCTGGGCTGGCAAGACATACTGTGTTCGAGCT
GTGTAGAGGCCTGCGCAGAGTCTGGATGACTGTCTGGGCATCCTTGTTCGGCTACACACATCCTGATAGG
CATCCCGTGTATTTCTTTAAATCCGCCTGCCCCCCGAATAGCGTACCCGTGGACGCAGCCGGGGCTCCTT
TCGACGATGATGATTACCTCGATTACAGGGACGAGCGCGATACCGAAGAGGACGAAGATGGCAAGGAGGA
TAAGAACAATGTCCCCGGAAATGGCGTCTTCCAAAAAACGACTAGCAGCGTGGACACAAGCCCCCCCTAC
TGCCGCTGTAAAGGAAAGCTGGGACTTCGCATTATCACCCCGTTCCCTGCATGCACCATCGCCGTCCACC
CCAGTGTACTGCGAGCTGTCGCTCAAGTGCTCAATCACGCCGTGTGCCTTGACGCTGAACTGCACACCTT
GCTTGACCCCATTTCTCATCCGGAATCTAGCCTCGACACGGGAATCTATCATCATGGGCGGTCTGTGCGG
TTGCCTTACATGTATAAGATGGACCAGGACGATGGGTATTTCATGCACAGAAGACTCCTTCCTCTGTTCA
TCGTTCCAGACGCATATCGAGAACATCCTCTTGGTTTTGTAAGGGCTCAGTTGGATCTGCGAAACTTGCT
TCACCATCATCCCCCCCACGATCTGCCAGCACTGCCACTCTCTCCACCCCCCCGCGTCATTCTGTCCGTG
AGGGACAAAATCTGCCCGAGCACAGAAGCCAATTTCATTGAGACTCGCAGCCTCAATGTGACAAGATACC
GAAGACGGGGACTTACTGAAGTGCTGGCCTACCACCTGTACGGCGGGGATGGAGCGACAGCTGCCGCTAT
TAGTGATACCGATCTCCAAAGACTGGTGGTGACACGGGTGTGGCCGCCACTGCTCGAACATCTCACCCAA
CATTACGAACCTCATGTGTCCGAACAGTTCACCGCTCCTCACGTGCTGCTTTTCCAACCCCATGGGGCCT
GCTGTGTTGCTGTTAAGAGGCGAGACGGCGCAAGAACCAGAGACTTCAGATGCTTGAACTATACGCATAG
GAATCCCCAAGAGACTGTACAGGTATTTATTGACTTGAGAACAGAGCATTCCTACGCCTTGTGGGCCAGC
TTGTGGTCACGCTGCTTTACTAAAAAGTGCCATAGCAACGCAAAAAATGTGCACATTTCAATCAAGATCC
GCCCTCCAGATGCTCCCATGCCTCCCGCAACTGCCGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101249 representing YP_081518
Red=Cloning sites Green=Tags

MTLVLFATEYDSAHIVANVLSQTPTDHCVFPLLVKHQVSRRVYFCLQTQKCSDSRRVAPVFAVNNETLQL
SRYLAARQPIPLSALIASLDEAETRPLYRHLFRTPVLSPEHGGEVREFKHLVYFHHAAVLRHLNQVFLCP
TSPSWFISVFGHTEGQVLLTMAYYLFEGQYSTISTVEEYVRSFCTRDLGTIIPTHASMGEFARLLLGSPF
RQRVSAFVAYAVARNRRDYTELEQVDTQINAFRERARLPDTVCVHYVYLAYRTALARARLLEYRRVVAYD
ADAAPEAQCTREPGFLGRRLSTELLDVMQKYFSLDNFLHDYVETHLLRLDESPHSATSPHGLGLAGYGGR
IDGTHLAGFFGTSTQLARQLERINTLSESVFSPLERSLSGLLRLCASLRTAQTYTTGTLTRYSQRRYLLP
EPALAPLLERPLPVYRVHLPNDQHVFCAVASETWHRSLFPRDLLRHVPDSRFSDEALTETVWLHDDDVAS
TSPETQFYYTRHEVFNERLPVFNFVADFDLRLRDGVSGLARHTVFELCRGLRRVWMTVWASLFGYTHPDR
HPVYFFKSACPPNSVPVDAAGAPFDDDDYLDYRDERDTEEDEDGKEDKNNVPGNGVFQKTTSSVDTSPPY
CRCKGKLGLRIITPFPACTIAVHPSVLRAVAQVLNHAVCLDAELHTLLDPISHPESSLDTGIYHHGRSVR
LPYMYKMDQDDGYFMHRRLLPLFIVPDAYREHPLGFVRAQLDLRNLLHHHPPHDLPALPLSPPPRVILSV
RDKICPSTEANFIETRSLNVTRYRRRGLTEVLAYHLYGGDGATAAAISDTDLQRLVVTRVWPPLLEHLTQ
HYEPHVSEQFTAPHVLLFQPHGACCVAVKRRDGARTRDFRCLNYTHRNPQETVQVFIDLRTEHSYALWAS
LWSRCFTKKCHSNAKNVHISIKIRPPDAPMPPATAV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2838 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081518
RefSeq ORF 2838 bp
Locus ID 3077484
MW 107.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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