UL87 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101267

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for protein UL87 [Human herpesvirus 5], codon optimized for human cell expression, YP_081535



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CNY 14,160.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101267 represents NCBI reference of YP_081535 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCGCCGCTCCCCGGCGGCTCGGATGCGACGCCCTGATTGTCGTCGGGGGGAGCGCCATGCCAC
GGAGAGTCCTCCATGTGCCCGTGCATGTCCGGGCATGCAACCTGACACAGGAACTCTCCACCGGCGAGGA
CGCACGGTTTTGCAGACCCAGACCTGTCAACGTCGAACGGGTCAGAGCAGTCTTCGCAGCCCTCTATCGG
GCATGCCCCATCCACGTGAGAACTGAGCCAGAGCGGGTGAAGCTCGTCCTGGGACGGCTCCTCCTGGGAC
CTGTCGCAGTGCCATGCTTCTGCGATGGCGAAGTGGAAGGCCACGGCGAGCACCTGGTGCCTACCACACA
ATTCTGCCGGGGGCCCCTCCTGTACGTGCACCGGCGGTGTTGCTGCGGCAGCGTCACCGCCGGCCGGGCC
CTGAGCTATCACGTGCTGGAGAACCACGTCGCCACCCACGTGCTGCGGGGCCTGCTGAGCCTGACCGAGT
GGAACCGGGAGCTGCCCAGCCTGTTTTGCGACTGCCCCGGGGGCGGCGGCGCCTCCGGAACCGAGGAACG
GTACGCCATGGCCTGCCTGCCCCGGGACCTGAGCCTCCATCTCGACGACTACCCATACCTGATGGTCGAA
ATCGGGCGGGTGCTCAGCGTCTCCGAGGTCGACGATTACGTGACCGCCGTCAGCGGGTATCTGGGGGAGG
CCGCCGCCCCCCGGATCCAGGTGCATTACAAGCTCCTGTTCGGCCTGAACGTGAGACCCCAAGCCCCCTG
CGCCCTGGACGCCACCCGGGACTTCTTTCTGCTGGAGCTGCAGAAGCTCTGGCTCGGCGTCGAATACCAC
CACGAGGTGACCAGCGAATTCTTCGGCAGAGTGCTCGCCCAGCTGCACCGGGACCGGGCCCGGGTGATGA
TGGCCCTGCGGCTGCCCGAGCAGACCGTGTGCCACCTGAGCACCTTCGTGCTGTCCCGGTTCAAGCGGCA
GGTGCTCTACTTCAAGCTGCAGGTGAGCTACGGGAAGTGCCGGACCGGCCACGCCGACCGGAGCGGCGGC
GGCGGCAACGGCGGAAACCAGGGCCATCACAACCTGCTGTGCTACCGGCGGCTGTCCGTGACCTTCGCCG
ACACCGACACTGTCTGGCGGAACCTGTTCTACGTGTACTACGAGCTCGCCCGGGACCTGGGCAGCCACGG
CACCGAGAACCGGCCCGTCAACCGGGGCTATGGGGTCAGCTGCGCCCCTCGGACCAGCCGGCTGAGCCCC
AGCGAGAGCACAGTCGTGAGCGCCAACGGCCACGCCCTGAGCTCCACAGCCCTGCCCACCACCAGCGCCG
GGCACAAGCTCTCCCTGCCCCGGGACCCCGCCGCCGACCGGGTCCGGCGGTACGTGTGCATCATCTCCCG
GCTGATGTACGCCCGGTACGGCGAGCGGTGGCGGAAGCACCGGCAGCGGAGATCCGAAACCGGCGAGGAG
GAGGAGGAGGAAACTCTGGAGAGCGGCGAGACAGATGCAACCCCCCCCTTTGACTTCACCGGCCAACAGC
TGAGACGGGCCTACCAGGAGCACAGACGGCGGAAGCACCTGGCAGTGCAACGGTACGCCCCTTGCCGGCG
GAAACTGATCGGCGGGATGGAGTTTGCCGAGGTCACCGGGGTGAGCCTGGACCGGATCGCTGTGAATGCC
TTCAACACCAACCGGGTCATCAACATGAAGGCAGCCCTGTCCAGCATCGCTGCCAGCGGCCTGGGGGTGC
GGGCTCCCCGGCTCCCCAAGAACATGACCCACAGCTTCGTGATGTATAAGCACACTTTCAAGGAGCCCGC
CTGCACCGTCTCCACCTTCGTCTCCAACGACGCCGTCTACATCAATAGCCTCAACGTGAACATCCGGGGC
TCCTACCCCGAGTTTCTGTACAGCCTGGGCGTGTACCGGCTGCACGTGAACATCGACCATTTCTTCCTGC
CCGCCGTGGTGTGTAACAGCAATTCCAGCCTGGACGTGCACGGCCTCGAGGACCAAGCCGTCATCCGGAG
CGAGCGGAGCAAGGTCTACTGGACCACCAACTTCCCTTGCATGATCTCCCACACCAACAATGTGAACGTG
GGCTGGTTCAAGGCCGCCACCGCCATCGTCCCCCGGGTGTCCGGCGCCGATCTGGAGGCAATCCTGCTCA
AAGAGCTCAGCTGCATCAAGAACATGCGGGACGTCTGCATCGACTACGGCCTGCACCGGGTCTTTACCCA
ACTCGAGCTGCGGAATTCCTATCAGATCCCCTTTCTGGCCAAGCAGCTCGTGCTCTTCCTGAGAGCCTGT
CTGCTGAAGCTGCACGGGCGGGAAAAGCGGCTCCAGCTCGACCGGCTCGTGTTCGAGGCCGCCCAGCGGG
GGCTGTTCGACTACTCCAAGAACCTGACCGCCCACACCAAAATCAAACACACCTGCGCTCTCATCGGCTC
CCGGCTGGCCAACAATGTCCCCAAGATCCTGGCACGGAACAAGAAGGTCAAGCTCGACCATCTCGGGCGG
AACGCCAACGTGCTGACTGTGTGCCGGCACGTGGAGGCCCACAAGATCCCCCGGACCCGGCTGAAGGTCC
TGGTCGAAGTCCTCGGGGTGCTGCAGAGCATCAGCGGCACACCCCACACTCGGGAGGTGATCCACCAAAC
CCTCTTCCGGCTGTGCAGCGCAGCTGCCGCCACCAGCGGACTGTGTAGCAGCCCCCCCCCCCTCTGCGTG
AGCAGCAGCAGCAGCGTGCCCTCCGTGCCCACTAGCGTCAGCGTGGATGGGAGCAGCGAGCCAACTTCCC
CTCGGGTGCGGTTTGCCTCCCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101267 representing YP_081535
Red=Cloning sites Green=Tags

MAGAAPRRLGCDALIVVGGSAMPRRVLHVPVHVRACNLTQELSTGEDARFCRPRPVNVERVRAVFAALYR
ACPIHVRTEPERVKLVLGRLLLGPVAVPCFCDGEVEGHGEHLVPTTQFCRGPLLYVHRRCCCGSVTAGRA
LSYHVLENHVATHVLRGLLSLTEWNRELPSLFCDCPGGGGASGTEERYAMACLPRDLSLHLDDYPYLMVE
IGRVLSVSEVDDYVTAVSGYLGEAAAPRIQVHYKLLFGLNVRPQAPCALDATRDFFLLELQKLWLGVEYH
HEVTSEFFGRVLAQLHRDRARVMMALRLPEQTVCHLSTFVLSRFKRQVLYFKLQVSYGKCRTGHADRSGG
GGNGGNQGHHNLLCYRRLSVTFADTDTVWRNLFYVYYELARDLGSHGTENRPVNRGYGVSCAPRTSRLSP
SESTVVSANGHALSSTALPTTSAGHKLSLPRDPAADRVRRYVCIISRLMYARYGERWRKHRQRRSETGEE
EEEETLESGETDATPPFDFTGQQLRRAYQEHRRRKHLAVQRYAPCRRKLIGGMEFAEVTGVSLDRIAVNA
FNTNRVINMKAALSSIAASGLGVRAPRLPKNMTHSFVMYKHTFKEPACTVSTFVSNDAVYINSLNVNIRG
SYPEFLYSLGVYRLHVNIDHFFLPAVVCNSNSSLDVHGLEDQAVIRSERSKVYWTTNFPCMISHTNNVNV
GWFKAATAIVPRVSGADLEAILLKELSCIKNMRDVCIDYGLHRVFTQLELRNSYQIPFLAKQLVLFLRAC
LLKLHGREKRLQLDRLVFEAAQRGLFDYSKNLTAHTKIKHTCALIGSRLANNVPKILARNKKVKLDHLGR
NANVLTVCRHVEAHKIPRTRLKVLVEVLGVLQSISGTPHTREVIHQTLFRLCSAAAATSGLCSSPPPLCV
SSSSSVPSVPTSVSVDGSSEPTSPRVRFASR

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2823 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081535
RefSeq ORF 2823 bp
Locus ID 3077465
MW 104.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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