UL97 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101276

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument serine/threonine protein kinase [Human herpesvirus 5], codon optimized for human cell expression, YP_081544



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CNY 10,640.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101276 represents NCBI reference of YP_081544 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGCGCCCTGAGATCCAGAGCACGGAGCGCCTCCCTCGGGACCACCACCCAAGGGTGGGATCCTC
CTCCACTCAGACGGCCAAGCCGGGCAAGACGGAGACAGTGGATGAGAGAGGCAGCTCAGGCAGCTGCTCA
AGCAGCAGTCCAAGCAGCACAGGCCGCAGCCGCACAGGTCGCCCAGGCCCACGTGGACGAGGACGAAGTG
GTGGACCTCATGACCGATGAGGCCGGCGGCGGCGTGACCACCCTGACCACCCTGTCCAGCGTGTCCACCA
CCACTGTGCTCGGCCACGCCACATTTTCCGCCTGTGTGCGGAGCGACGTCATGCGGGATGGCGAGAAGGA
GGACGCCGCTAGCGACAAGGAGAACCAGCGGCGGCCCGTGGTGCCCTCCACCAGCAGCCGGGGGTCCGCC
GCCTCCGGGGACGGCTATCACGGGCTCCGGTGCCGGGAAACCAGCGCCATGTGGAGCTTCGAGTACGACC
GGGACGGCGACGTCACCAGCGTCCGGCGGGCCCTCTTCACCGGCGGCAGCGATCCCAGCGACAGCGTGAG
CGGGGTGCGGGGGGGGCGGAAACGGCCCCTGCGGCCCCCCCTCGTGTCCCTGGCCCGGACACCACTGTGC
CGGCGGCGGGTCGGCGGCGTCGACGCAGTGCTGGAGGAGAACGACGTGGAACTCCGGGCCGAGAGCCAGG
ACAGCGCCGTGGCCTCCGGGCCCGGGCGGGTGCCCCAGCCCCTCTCCGGGAGCAGCGGCGAGGAGTCCGC
AACCGCCGTCGAGGCCGACAGCACCAGCCACGACGATGTGCATTGCACCTGCTCCAACGACCAGATCATC
ACCACTTCCATCCGGGGCCTGACCTGCGACCCTCGGATGTTCCTGCGGCTGACACACCCCGAGCTGTGCG
AGCTCAGCATCTCCTACCTGCTGGTGTACGTGCCCAAGGAGGACGACTTCTGCCACAAGATCTGCTACGC
CGTGGATATGAGCGACGAGTCCTACCGGCTGGGCCAGGGCTCCTTCGGCGAGGTGTGGCCCCTGGACCGG
TACCGGGTGGTGAAGGTCGCTCGGAAGCACAGCGAGACCGTCCTCACCGTCTGGATGTCCGGACTGATCC
GGACCCGGGCCGCTGGCGAGCAGCAGCAGCCCCCCAGCCTCGTCGGGACAGGCGTGCACCGGGGCCTCCT
GACCGCCACCGGCTGTTGCCTGCTCCACAACGTGACCGTCCACCGGCGGTTCCACACCGACATGTTCCAC
CACGACCAGTGGAAGCTGGCCTGCATCGACAGCTACCGGCGGGCCTTCTGCACCCTCGCCGACGCCATCA
AGTTCCTCAACCACCAATGCCGGGTCTGCCACTTCGACATCACCCCCATGAACGTGCTGATCGACGTCAA
CCCTCACAACCCCTCCGAGATCGTGCGGGCCGCCCTGTGCGATTACTCCCTCAGCGAGCCCTACCCCGAC
TACAACGAGCGGTGCGTGGCCGTGTTCCAGGAGACCGGCACCGCCAGACGGATCCCCAACTGTTCCCATC
GGCTGCGGGAGTGCTACCACCCCGCCTTTCGGCCCATGCCCCTCCAGAAGCTGCTCATCTGCGACCCCCA
CGCTCGGTTCCCCGTCGCCGGCCTGCGGCGGTACTGCATGTCCGAGCTGAGCGCCCTGGGCAACGTGCTG
GGCTTCTGCCTCATGCGGCTGCTGGACCGGCGGGGGCTCGATGAGGTGCGGATGGGCACCGAAGCCCTGC
TCTTCAAGCACGCCGGCGCCGCATGCCGGGCCCTGGAGAATGGCAAGCTCACCCACTGCAGCGACGCTTG
CCTGCTGATCCTCGCCGCCCAAATGTCCTACGGCGCCTGCCTCCTCGGCGAGCACGGAGCCGCCCTCGTG
AGCCACACCCTGCGGTTCGTGGAGGCCAAAATGAGCAGCTGTCGGGTGCGGGCCTTTCGGCGGTTCTACC
ATGAGTGCTCCCAGACCATGCTGCATGAGTACGTGAGAAAGAACGTGGAGCGGCTGCTGGCCACCTCCGA
TGGGCTCTACCTCTACAACGCCTTCCGGCGGACCACCAGCATCATCTGTGAGGAAGACCTGGACGGCGAC
TGTCGGCAACTCTTTCCAGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101276 representing YP_081544
Red=Cloning sites Green=Tags

MSSALRSRARSASLGTTTQGWDPPPLRRPSRARRRQWMREAAQAAAQAAVQAAQAAAAQVAQAHVDEDEV
VDLMTDEAGGGVTTLTTLSSVSTTTVLGHATFSACVRSDVMRDGEKEDAASDKENQRRPVVPSTSSRGSA
ASGDGYHGLRCRETSAMWSFEYDRDGDVTSVRRALFTGGSDPSDSVSGVRGGRKRPLRPPLVSLARTPLC
RRRVGGVDAVLEENDVELRAESQDSAVASGPGRVPQPLSGSSGEESATAVEADSTSHDDVHCTCSNDQII
TTSIRGLTCDPRMFLRLTHPELCELSISYLLVYVPKEDDFCHKICYAVDMSDESYRLGQGSFGEVWPLDR
YRVVKVARKHSETVLTVWMSGLIRTRAAGEQQQPPSLVGTGVHRGLLTATGCCLLHNVTVHRRFHTDMFH
HDQWKLACIDSYRRAFCTLADAIKFLNHQCRVCHFDITPMNVLIDVNPHNPSEIVRAALCDYSLSEPYPD
YNERCVAVFQETGTARRIPNCSHRLRECYHPAFRPMPLQKLLICDPHARFPVAGLRRYCMSELSALGNVL
GFCLMRLLDRRGLDEVRMGTEALLFKHAGAACRALENGKLTHCSDACLLILAAQMSYGACLLGEHGAALV
SHTLRFVEAKMSSCRVRAFRRFYHECSQTMLHEYVRKNVERLLATSDGLYLYNAFRRTTSIICEEDLDGD
CRQLFPE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2121 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081544
RefSeq ORF 2121 bp
Locus ID 3077517
MW 78.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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