UL47 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101236

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 5], codon optimized for human cell expression, YP_081505



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CNY 14,820.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101236 represents NCBI reference of YP_081505 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCTCGCAGGACGGTCGATTTTAAGAAGCTGATCGAGCAATTGCGCGCGCGCGCTACCGACAAGG
CCGAGGCTCTGAATACCGTTAGCCAACTCGAGATCGGGGCAGTGGATGCCCAGGACGTGACGGCTAGTGC
TGTCCGGGCTTTCGTTGGTGCGCTTCCAAGCAGCGGTTACCACTTCGGGTTCGTCAGACAGAACGTGGTT
TTTTACCTGCTTTCACACGCCACGGTGCAGACCGCAAGGGATCCCTTGTATGCCGCAGAACAGCTGCACG
AACAATTGGATAGGTTTTTGCGGCACCAGCATGACGGCGGGGGAGATGAAGATAGGCTGCCCTTTTATCA
TAACGGGGCGACACTTACTGCTTTCCAAAAACTTCTGCAGACCTTGAGGGAAATTCAGACCGTGATTGCC
GAGCAGTCAGGAGGAACAGCTGCGGCCGCCGACCTGATTGCTTCAAACAATGCTTCTACAGAACGCCGGG
GGAAGAAGGGTGGCTCTTCCAGCGGCGGTCAGCAGCCCCTGGTCCGGAGGGTGATTACTCAACTGGAGAC
TGCCGCTACCGAGGCTAGGCCATACGTTAATTGTCGCGCGGTAGCTGAGCTTCTCGACCTCACTTACCAG
CGGTTGATATATTGGGCGTGCACACTTATGCCATACGTGCTCTTTAGGCGGGATACTGACACCGAACTGG
ACACTGTGCTGCTGATGCACTTTTTCTACACTCACTATCGCTCAGTCAACGGTGACCTGGCAGTGGAGTT
TCAGAACTACGTAAAAAATTCCGTGAGACACATGAGCTCATTTGTTTCTAGCGACATCGACGGGGATCAG
AAGCCGGGAGCAGAGCACATGCGGGACGTCTCCTATAAACTCTTTGTGGGCAATCTGCAGGCTCGCGACG
CCAGCGGTCTGATGTTCCCGATCATCAGCACGAGGATCAGCACGGTTAACCTGTATCTCTCCCCTGAACG
GATGTTCTTCCACCCCGGCCTGATTTCTAGGCTCCTGTCCGAAGAAGTGAGCCCTCGCGCGAACTTGGAC
GCTTACGCCAGGGTCTGCGACCGCGTGCTCGAGGATCATCTGCATACCCCACGGCGGGTTCAGAGGCTTT
TGGACTTGACACAGATGGTGATGCGACTGGTCGAGCTCGGTTTCAATCACGACACTTGCGCTGCATATGC
TCAGATGGCACTGATTCAACCTGCTTCTCAAAAAAGTAGTCTGTTTGTGAGTGAAATCCGAGAAAAGCTT
ATTCAGATCATTTATAATTTTTACACCTTCTTTATGTGTCTTTATGTGTATTCCCCTACCTTTCTGTTCG
ATCACCGGAGACGACTCATTCTCGAACAGCATCGCTCTACCCTGATCGGCAGCAAAGAGGAGCTTCAGCA
CGTCTGGAGTAATGTTACTTTGAACGTGAACACCCACTTCGCGGTGCAGTATACAGAAGAAGACTTTGAA
GCTCACACCAAAGGAGCTACCGAGGCAGAAAGGGAGTACCTTTACCGAGACCTCCACTCAAAATGGGGCG
TGCATCTTTTCACTCTTCGACCTTCTCGCGGGGCCGCTGGGGCAGCAAGTCCACTGCCTCCACTTGACGG
CGTCACGAGGTCAGACATTCTGCGGGAATGTGCTCTGGTCAACCTGAACGAGGGCCGAGTCAACTATGCC
TCCTTGCTGGCATTCTCACACCACCCAGAATTTCCTTCCATTTTCGCTCAGCTCGTGGTGGTCACCGAGT
TCAGTGAAATTTTTGGAATACCGCAGGGACTTTTCCAGGCAGTCGGTAGCCCGAGACTGTTTGCTCTCAT
TCAGCTTTGCCGGGTCCTCTTGCCAGAGCAGGTGACTCTCTACCAGAATCTGGTGAGTATTTATAACTTG
ACCACGTTCGTGAAACATATCGACGCTGCCGTTTTCAAGACTGTCAGGGACTGTGTGTTCGACATCGCAA
CTACCCTCGAACACCTCTCAGGCGTCCCCGTTACCCCGAACGTCGACCTGTTGGCCGAGCTGATGGCCCG
GTCTGTGGCGCACAACCTTTACACCACAGTCAATCCCCTTATCGAAGATGTGATGCGATCTTCCGCGGGG
TCTCTGAGGAACTACCTGCGCCACACTCGCCTCTGCTTTGGTCTGGCTCGCGGGAGGGCCAGGCTTAGCG
AGGACGGCGTCACCGTGTACGTGGAGGTGCAGGGCCAGTACGGGCTGCGCGTACCCACCACGCGCTTTGT
TGAGCAGCTGAGAGAGCTGGTGCGGCGGGACAGGTTGCTGGCTGAGAACCTTAGGGGACTCAACGAACGG
CTGCTCTCCGTAAGGGTCAGGGTGCGCCAAATCTCAAGTGATACTGAGGAGGTTTCAAGGCACGCAAAGG
GTCACCGGACTGTCGCCCAGATGAGCAAAGCGCTTAAAAAGACCGCTTCTAAAATCAAAATGTTGGAGAC
TCGGGTTACTCTGGCCCTCGAACAAGCCCAGCGGTCAAACGGAGCGGTCGTGACCGCGGTACAGCGCGCA
CTGGCAGTGTTCGACGTGCTCAGCAGAGAAAACCTGGAGAGGCGCGGCGCACAGCTTTGTCTTACAGAGG
CAACATCTCTCCTCCACAGGCATCGCGCACTCGCCCCTATGACCTGGCCAGCTGGTACAGGCGTAGCCGC
AGCTGCCGAGGCAGACGGAGCCTTGCGAGAGTTCCTGGAGGCACCTTGGGAAAGCGCGCCACAGCCCCCC
CGCCTGAGAATGACGCCAGACACCGACCATGAGGAATCCACCGCCGGGGCAACCTCCGTCCCCGAAGTAT
TGGGCGCCCGCTATGAACCAGCCCATTTGGCAGCTTCCGACCTTCTCAATTGGTATATTGTACCCGTCTC
ACAGGCCCAACAAGATATTTTGTCTAGCATAGATCCTCCCGCTGGTAGCACATCCGTTTCCCTCCCACCT
GCCTCACCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101236 representing YP_081505
Red=Cloning sites Green=Tags

MMARRTVDFKKLIEQLRARATDKAEALNTVSQLEIGAVDAQDVTASAVRAFVGALPSSGYHFGFVRQNVV
FYLLSHATVQTARDPLYAAEQLHEQLDRFLRHQHDGGGDEDRLPFYHNGATLTAFQKLLQTLREIQTVIA
EQSGGTAAAADLIASNNASTERRGKKGGSSSGGQQPLVRRVITQLETAATEARPYVNCRAVAELLDLTYQ
RLIYWACTLMPYVLFRRDTDTELDTVLLMHFFYTHYRSVNGDLAVEFQNYVKNSVRHMSSFVSSDIDGDQ
KPGAEHMRDVSYKLFVGNLQARDASGLMFPIISTRISTVNLYLSPERMFFHPGLISRLLSEEVSPRANLD
AYARVCDRVLEDHLHTPRRVQRLLDLTQMVMRLVELGFNHDTCAAYAQMALIQPASQKSSLFVSEIREKL
IQIIYNFYTFFMCLYVYSPTFLFDHRRRLILEQHRSTLIGSKEELQHVWSNVTLNVNTHFAVQYTEEDFE
AHTKGATEAEREYLYRDLHSKWGVHLFTLRPSRGAAGAASPLPPLDGVTRSDILRECALVNLNEGRVNYA
SLLAFSHHPEFPSIFAQLVVVTEFSEIFGIPQGLFQAVGSPRLFALIQLCRVLLPEQVTLYQNLVSIYNL
TTFVKHIDAAVFKTVRDCVFDIATTLEHLSGVPVTPNVDLLAELMARSVAHNLYTTVNPLIEDVMRSSAG
SLRNYLRHTRLCFGLARGRARLSEDGVTVYVEVQGQYGLRVPTTRFVEQLRELVRRDRLLAENLRGLNER
LLSVRVRVRQISSDTEEVSRHAKGHRTVAQMSKALKKTASKIKMLETRVTLALEQAQRSNGAVVTAVQRA
LAVFDVLSRENLERRGAQLCLTEATSLLHRHRALAPMTWPAGTGVAAAAEADGALREFLEAPWESAPQPP
RLRMTPDTDHEESTAGATSVPEVLGARYEPAHLAASDLLNWYIVPVSQAQQDILSSIDPPAGSTSVSLPP
ASP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_006273
ORF Size 2949 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_006273.2, YP_081505
RefSeq ORF 2949 bp
Locus ID 3077521
MW 110.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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