BXLF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101160

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BXLF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401700



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CNY 10,640.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101160 represents NCBI reference of YP_401700 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCTTCTGTGCGTGTTTTGCCTGGTCCTGTTGTGGGAAGTGGGAGCGGCTAGCTTGAGTGAGGTGA
AGCTTCACCTTGACATTGAGGGCCATGCCTCCCATTACACTATCCCCTGGACCGAGCTCATGGCTAAGGT
GCCCGGGCTTTCTCCTGAGGCCCTGTGGCGCGAAGCAAACGTGACTGAGGATTTGGCGTCCATGCTCAAT
CGCTATAAGCTGATCTACAAAACCAGCGGAACGCTGGGAATAGCCCTCGCCGAACCGGTGGACATCCCTG
CAGTAAGTGAGGGCTCCATGCAGGTTGACGCCTCAAAAGTGCACCCAGGCGTGATCTCTGGGCTGAACTC
TCCCGCCTGCATGCTGAGCGCACCGTTGGAGAAGCAGCTCTTTTATTATATTGGAACAATGCTGCCCAAC
ACCCGGCCCCACTCATACGTCTTCTACCAGCTGAGATGCCATTTGAGCTACGTCGCTTTGAGCATCAACG
GGGACAAATTCCAGTACACTGGCGCCATGACCTCCAAGTTTCTGATGGGAACCTACAAAAGGGTGACTGA
AAAAGGAGATGAGCACGTGCTCAGCCTGGTCTTCGGCAAGACCAAAGACTTGCCAGATCTGCGAGGCCCC
TTCAGTTACCCAAGTCTTACCTCCGCACAAAGTGGCGACTATTCATTGGTTATCGTAACTACCTTCGTTC
ACTACGCTAATTTCCATAATTACTTTGTCCCTAATCTCAAAGACATGTTTTCACGGGCTGTGACGATGAC
CGCGGCAAGCTATGCACGGTATGTACTGCAGAAACTCGTCCTGCTGGAGATGAAGGGCGGCTGCCGCGAA
CCTGAATTGGACACAGAGACCTTGACAACCATGTTCGAAGTGTCTGTCGCCTTCTTTAAGGTGGGCCACG
CGGTTGGCGAGACTGGCAATGGTTGTGTGGATTTGAGATGGCTTGCAAAGTCTTTCTTCGAACTGACCGT
GCTGAAAGATATCATAGGCATTTGCTACGGTGCAACGGTCAAGGGCATGCAGTCCTACGGCCTGGAGAGA
CTGGCGGCTATGCTGATGGCAACCGTGAAGATGGAGGAGTTGGGACATCTGACAACAGAAAAACAGGAGT
ACGCCTTGAGGTTGGCTACCGTTGGATACCCCAAGGCCGGAGTCTACAGCGGACTGATTGGGGGCGCCAC
TTCAGTCCTTCTGAGTGCTTACAACCGCCATCCTCTCTTCCAGCCACTCCACACAGTGATGCGCGAAACC
CTTTTCATCGGTAGCCACGTGGTCCTGCGCGAACTTCGCCTTAACGTCACCACTCAGGGGCCTAACCTGG
CTCTCTACCAGCTTCTGTCAACGGCACTCTGCTCAGCCCTCGAAATCGGGGAGGTTCTCCGGGGGTTGGC
CCTGGGCACTGAGTCCGGGCTGTTCTCCCCTTGTTACTTGTCTCTGCGATTTGACCTGACCAGAGATAAA
TTGCTTAGTATGGCACCCCAGGAGGCCACCCTTGATCAAGCTGCAGTGTCCAACGCAGTCGACGGGTTCC
TGGGGCGGCTGAGCTTGGAAAGAGAGGACCGCGACGCTTGGCATCTTCCAGCTTATAAGTGTGTGGACAG
ACTGGACAAAGTGTTGATGATCATCCCCCTGATCAACGTGACCTTTATCATCAGTAGTGATCGCGAGGTA
CGGGGTAGCGCGCTCTATGAAGCAAGCACCACCTACCTCTCCTCATCCTTGTTTCTGTCTCCCGTCATTA
TGAATAAGTGCTCCCAAGGTGCAGTCGCAGGAGAACCCCGGCAAATTCCAAAAATCCAGAATTTTACCAG
GACTCAAAAAAGTTGTATATTTTGTGGATTCGCCTTGCTCAGCTACGATGAAAAGGAGGGATTGGAAACC
ACCACCTATATTACGAGTCAGGAAGTACAGAACTCCATTCTGTCTAGTAACTACTTCGACTTCGATAACC
TGCACGTGCATTACCTTCTTCTGACCACGAACGGCACTGTTATGGAAATTGCCGGTCTTTACGAGGAACG
AGCTCACGTCGTCTTGGCTATAATCCTGTATTTTATTGCATTTGCTCTCGGCATTTTCCTGGTCCATAAA
ATCGTCATGTTCTTTCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101160 representing YP_401700
Red=Cloning sites Green=Tags

MQLLCVFCLVLLWEVGAASLSEVKLHLDIEGHASHYTIPWTELMAKVPGLSPEALWREANVTEDLASMLN
RYKLIYKTSGTLGIALAEPVDIPAVSEGSMQVDASKVHPGVISGLNSPACMLSAPLEKQLFYYIGTMLPN
TRPHSYVFYQLRCHLSYVALSINGDKFQYTGAMTSKFLMGTYKRVTEKGDEHVLSLVFGKTKDLPDLRGP
FSYPSLTSAQSGDYSLVIVTTFVHYANFHNYFVPNLKDMFSRAVTMTAASYARYVLQKLVLLEMKGGCRE
PELDTETLTTMFEVSVAFFKVGHAVGETGNGCVDLRWLAKSFFELTVLKDIIGICYGATVKGMQSYGLER
LAAMLMATVKMEELGHLTTEKQEYALRLATVGYPKAGVYSGLIGGATSVLLSAYNRHPLFQPLHTVMRET
LFIGSHVVLRELRLNVTTQGPNLALYQLLSTALCSALEIGEVLRGLALGTESGLFSPCYLSLRFDLTRDK
LLSMAPQEATLDQAAVSNAVDGFLGRLSLEREDRDAWHLPAYKCVDRLDKVLMIIPLINVTFIISSDREV
RGSALYEASTTYLSSSLFLSPVIMNKCSQGAVAGEPRQIPKIQNFTRTQKSCIFCGFALLSYDEKEGLET
TTYITSQEVQNSILSSNYFDFDNLHVHYLLLTTNGTVMEIAGLYEERAHVVLAIILYFIAFALGIFLVHK
IVMFFL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 2118 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_007605.1, YP_401700
RefSeq ORF 2118 bp
Locus ID 3783742
UniProt ID P03211
MW 78.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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