BBLF4 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101141

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BBLF4 [Human herpesvirus 4], codon optimized for human cell expression, YP_401681



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CNY 12,260.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101141 represents NCBI reference of YP_401681 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGAACCTCGCGCCCCCGAAGCCCTGAGCTCCACATTTATGCTCAATATGACTAGCGATGCTT
CAGTCAGGAGGATTGTTCGGAGGATCGGGACCTTGGCTCGCAGAAGGGTGCAACAGCTGCCGGACATGGA
GACGTTCTCCCCTGAATTCGACCCCGAACTTAGCGAACCCCCCTTCCTGCCTTTTAGCGCCTACGTCATT
ACCGGAACCGCTGGCGCTGGAAAGAGCACGTCTGTTTCATGTTTGCACCACACGATGGACTGTCTCGTAA
CCGGCGCTACCACAGTAGCTGCCCAGAACCTCTCACAGACCCTGAGGGCATACTGTCCCACAGTCTACTC
CGCATTTGGGTTTAAAAGCCGGCATATCAACATGACTCAAAGGGTATCTTCTCACGGCCGGAGCACAGAT
GCCGCGCTTGAAGAGCTCCAACGGAGAGACCTGGCCAAATATTGGCCTGTACTGAGTGACATCGCTGCGG
AGTTCAGGCGGACCAAACCAAGGGGCCTTTATAGTGGGGTATCTGGACCCGCTTTCGAAGTTCTCAGGGA
CATGCATCAAGGCCAGCTTTGGACAACAAATGTAATTGTGGTAGATGAGGCTGGTACCCTCTCCGTACAC
ATACTGACAGCAGTGGTATTTTGTTACTGGTTTTTCAACGCCTGGCTGAGAACACCCCTCTACCGACGAG
GAAGGATCCCCTGTATCGTTTGCGTGGGCAGCCCTACACAAACTGACGCCTTCCAGAGCAGTTTTAGCCA
CGAGACGCAGGTTAACAAGATACGAGAATGTGATAATATTCTGACATTCCTGGTGGGCAACCCCCGGGCA
GCAACCTACGTGGATGTGGCTAGGAATTGGGCTCTGTTCATCAATAACAAACGGTGCACTGATGTTCAGT
TTGGACACCTGATGAAAACTCTGGAATATGGGCTGGAGCTCAGCCCCGACATTTTGGCGTACGTGGACCG
ATTTGTGGTCCCACGCGCAGCTATCATGGATCCAGCACAGTACGTAGGTTGGACCAGACTGTTTTTGTCC
CATGCCGAAGTTAAAACATTCCTCACCACTCTGCATGCTACACTTAAGACCGCCGGTCAGGGACGCGCTG
CTAGGGGGACAGGTGGCGACGGGGGAGGCGTTACAATGTTTACCTGCCCTGTTGAGTGCGAAGTCTTCCT
GGATCCTCTGGCTCAATATAAAACCTTGGTTGGCCTCCCCGGTTTGACTGCACATACCTGGTTGCAGAAA
AATTATGCCCGGCTGGGAAATTACAGCCAATTCGCCGATCAAGATATGGTCCCAGTGGGCACCGAGCAGG
ATGAAGAACGGGTGAAAGTCACATACAACGTAACCTACGTTAAACATAGTAGCGTGTCAGTGAACTGTAA
AACCAAAAAGTCCATCTGCGGCTATACCGGTACATTTGGGGATTTCATGGATACTTTGGAAGCCGACAGT
TTCGTTGAGGCACATGGTCATGAACAGCCCGAGTATGTCTACAGCTTTCTCGCCCGGCTGATTTACGGCG
GCATTTACGCTTTCTCACACGGGGGTCATAGTCTGTGCGAGAATGGCGAGTACGTGGCAGAGCTGGGAGC
CGTTCCACTGCCCGGGCGGACCTGGGACCCTGAAGTGACCGCTGGAATGGAACTGGGAGAGTTGCCTCTG
GAGGTGGCATGGGACGGGGAGAGGTCTCCAGCGGCGGTGTTCTATGCTCGGGTGCTGGCTCCACCTGCCG
CGAATTCCGCACCGCTCTGCAGTCTGCTTAACATCTACAACGATCTGAGGGCTTACTTTCGGCAGTGTCT
CGATGTTGCAGTCCGGTACGGCGGGCGGGAGTTTAGGGACCTCCCCTTCTGTACCTTCACTAACAACATG
CTCATCCGCGATAACATCGAATTCACTTCCGATGAACCACTGCTGCACGGGCTTCTGGACTATGCCAGTA
CTACTGAGAATTACACCCTCCTGGGTTACACCCACCTGAACGTATTCTTTGGGATCAGGGGGAAACAGCA
GCCACAGGATGCCGGGTCCAGTCGGATGCCCCGGCTGATGGTGAAGGATGAAGCCGGGTTTGTGTGCTGC
CTGGAGCACAATACGAACAAGTTGTATGAGACCATCGAGGACAAAAGCCTCAACCTGTGCTCTATTAGGG
ACTACGGCATATCTTCCAAATTGGCCATGACCATCGCCAAGGCACAGGGCCTGAGCCTGAATAAGGTGGC
AATCTGTTTTGGCTCTCACAGAAACATTAAACCAGGCCACGTATATGTCGCCCTGTCCAGGGCGCGGCAC
TCCAACTGTGTGGTAATGGACCGGAATCCTTTGTCCGAAATGATCACCGGAGAAGGAAACCCCGCCAGTG
GATACATCGTGGACGCCTTGAAGAATTCTAGGGCTCTGCTCGTTTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101141 representing YP_401681
Red=Cloning sites Green=Tags

MAEEPRAPEALSSTFMLNMTSDASVRRIVRRIGTLARRRVQQLPDMETFSPEFDPELSEPPFLPFSAYVI
TGTAGAGKSTSVSCLHHTMDCLVTGATTVAAQNLSQTLRAYCPTVYSAFGFKSRHINMTQRVSSHGRSTD
AALEELQRRDLAKYWPVLSDIAAEFRRTKPRGLYSGVSGPAFEVLRDMHQGQLWTTNVIVVDEAGTLSVH
ILTAVVFCYWFFNAWLRTPLYRRGRIPCIVCVGSPTQTDAFQSSFSHETQVNKIRECDNILTFLVGNPRA
ATYVDVARNWALFINNKRCTDVQFGHLMKTLEYGLELSPDILAYVDRFVVPRAAIMDPAQYVGWTRLFLS
HAEVKTFLTTLHATLKTAGQGRAARGTGGDGGGVTMFTCPVECEVFLDPLAQYKTLVGLPGLTAHTWLQK
NYARLGNYSQFADQDMVPVGTEQDEERVKVTYNVTYVKHSSVSVNCKTKKSICGYTGTFGDFMDTLEADS
FVEAHGHEQPEYVYSFLARLIYGGIYAFSHGGHSLCENGEYVAELGAVPLPGRTWDPEVTAGMELGELPL
EVAWDGERSPAAVFYARVLAPPAANSAPLCSLLNIYNDLRAYFRQCLDVAVRYGGREFRDLPFCTFTNNM
LIRDNIEFTSDEPLLHGLLDYASTTENYTLLGYTHLNVFFGIRGKQQPQDAGSSRMPRLMVKDEAGFVCC
LEHNTNKLYETIEDKSLNLCSIRDYGISSKLAMTIAKAQGLSLNKVAICFGSHRNIKPGHVYVALSRARH
SNCVVMDRNPLSEMITGEGNPASGYIVDALKNSRALLVY

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 2427 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_007605.1, YP_401681
RefSeq ORF 2427 bp
Locus ID 3783685
UniProt ID P03211
MW 89.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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