BNRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101095

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BNRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401633



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CNY 19,950.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101095 represents NCBI reference of YP_401633 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGCGCGGGAGGGAGACCCAGATGCCGGTGGCCCGATACGGCGGCCCGTTCATTATGGTGCGGC
TGTTCGGGCAAGATGGTGAGGCAAACATTCAAGAGGAGAGACTGTACGAGCTGTTGTCTGACCCCCGCTC
CGCCCTGGGATTGGACCCCGGGCCTCTTATAGCCGAGAATCTCTTGTTGGTAGCTCTGCGCGGCACCAAC
AACGACCCAAGGCCTCAGAGGCAGGAGCGGGCCAGGGAACTGGCACTTGTGGGAATATTGCTGGGTAATG
GAGAACAGGGTGAACACCTTGGCACAGAAAGCGCCCTGGAGGCTAGCGGCAACAACTATGTGTACGCTTA
TGGGCCTGATTGGATGGCTCGGCCATCAACATGGTCCGCCGAAATACAGCAATTCCTTAGACTTCTTGGG
GCGACCTACGTGCTGAGGGTGGAGATGGGTCGGCAGTTCGGCTTCGAGGTTCACCGGTCCAGACCTAGCT
TTAGACAATTCCAGGCCATCAACCATCTGGTGCTCTTCGACAATGCCCTGAGAAAGTACGACAGCGGACA
GGTCGCTGCTGGGTTTCAACGCGCTCTTCTCGTGGCCGGGCCTGAGACCGCTGACACCCGACCGGACCTC
AGAAAATTGAATGAATGGGTGTTTGGCGGGAGAGCCGCCGGGGGTAGGCAGCTGGCCGACGAACTTAAGA
TCGTAAGCGCTCTTCGAGATACATACAGCGGCCACCTTGTACTGCAGCCAACAGAAACGCTGGACACTTG
GAAAGTCCTGTCTCGGGATACGCGGACTGCACATAGCTTGGAGCACGGCTTCATCCACGCCGCTGGGACT
ATACAGGCAAACTGTCCACAACTGTTCATGAGACGCCAACATCCAGGCCTGTTTCCTTTCGTGAACGCCA
TCGCTAGTAGCCTGGGATGGTACTACCAGACTGCAACAGGTCCTGGCGCAGATGCAAGGGCCGCTGCCCG
GAGGCAACAAGCCTTCCAGACCCGGGCTGCAGCAGAGTGCCACGCAAAGAGCGGGGTCCCTGTCGTGGCA
GGTTTCTATAGGACAATCAACGCTACTTTGAAGGGAGGCGAGGGCCTTCAGCCCACGATGTTTAATGGTG
AGCTGGGGGCCATTAAGCATCAGGCACTCGATACGGTGAGGTACGACTACGGACACTACCTGATTATGCT
CGGCCCATTCCAGCCATGGTCAGGGCTCACTGCCCCTCCCTGTCCTTACGCAGAATCATCTTGGGCTCAG
GCCGCTGTCCAGACAGCGCTGGAACTGTTTTCCGCCCTGTACCCCGCTCCCTGTATCTCTGGATACGCCC
GGCCTCCAGGCCCTTCTGCCGTGATCGAGCATCTCGGAAGTCTCGTCCCCAAGGGCGGGCTCCTGCTGTT
CCTTAGCCATCTCCCCGATGACGTGAAGGACGGACTGGGCGAGATGGGACCTGCCAGGGCTACCGGTCCG
GGCATGCAGCAGTTTGTCTCATCCTATTTTCTCAACCCTGCATGTAGCAATGTCTTTATTACAGTGCGGC
AGCGCGGGGAGAAGATCAACGGACGCACTGTGCTGCAGGCCCTGGGGCGAGCCTGCGACATGGCTGGATG
CCAGCACTATGTACTGGGATCTACTGTTCCCCTCGGGGGCCTGAACTTCGTGAACGACCTGGCCAGCCCT
GTGAGCACAGCCGAGATGATGGATGACTTCTCTCCATTCTTTACCGTCGAGTTTCCTCCAATACAAGAAG
AGGGCGCATCATCACCTGTTCCACTGGATGTGGATGAGTCAATGGACATAAGTCCAAGTTATGAACTGCC
TTGGCTGAGCCTGGAATCTTGCCTGACATCAATTCTGTCTCACCCAACCGTGGGATCTAAGGAACATTTG
GTTCGACACACCGATCGCGTGTCAGGTGGCCGGGTAGCTCAGCAGCCCGGCGTAGGGCCACTGGACTTGC
CCCTTGCAGACTACGCATTTGTGGCTCATAGTCAGGTGTGGACCCGCCCTGGAGGTGCACCACCACTTCC
ATATCGGACATGGGACCGAATGACTGAAAAGCTGCTCGTCAGCGCTAAGCCCGGAGGCGAAAATGTGAAA
GTGAGCGGCACTGTGATCACTCTGGGAGAGCAAGGGTATAAAGTTAGCCTGGATCTCCGCGAGGGAACGA
GACTCGCCATGGCCGAAGCCCTTTTGAACGCTGCATGTGCTCCAATATTGGATCCGGAAGACGTACTTCT
CACACTGCATCTGCATCTCGACCCCAGGCGCGCCGATAACAGCGCCGTGATGGAGGCGATGACCGCTGCC
AGCGATTATGCAAGGGGTCTTGGTGTTAAGCTGACGTTTGGATCAGCCTCCTGTCCTGAGACCGGGAGCT
CTGCCTCTAACTTCATGACCGTGGTGGCCTCCGTGTCAGCTCCCGGAGAATTCTCTGGCCCACTGATTAC
ACCTGTGTTGCAGAAGACCGGGTCCCTTCTGATTGCTGTGCGGTGTGGTGATGGGAAAATCCAGGGTGGG
TCACTTTTCGAGCAGCTTTTCTCTGACGTCGCCACCACTCCCCGAGCCCCCGAGGCCCTGAGCCTGAAAA
ACCTGTTCAGGGCCGTGCAGCAGCTCGTTAAGAGTGGGATCGTACTCAGCGGGCACGACATTTCAGACGG
AGGACTGGTCACCTGCCTGGTAGAGATGGCTCTGGCAGGCCAGCGCGGCGTAACCATCACCATGCCCGTC
GCCTCAGATTACCTGCCCGAGATGTTCGCCGAACACCCCGGCCTGGTGTTCGAAGTGGAAGAAAGATCCG
TGGGAGAGGTACTGCAGACTCTGCGGTCCATGAACATGTACCCCGCTGTGCTGGGGCGCGTGGGAGAACA
GGGACCCGACCAGATGTTCGAAGTCCAGCACGGCCCGGAGACAGTCCTGCGACAGAGCCTGCGGCTGCTG
CTCGGAACTTGGAGCTCATTTGCATCCGAGCAGTATGAATGCCTGAGACCTGACCGAATCAACCGGAGCA
TGCATGTAAGTGACTATGGCTACAACGAGGCCTTGGCTGTTTCACCGCTTACTGGCAAGAACCTCTCTCC
GCGGAGGTTGGTGACAGAGCCCGACCCAAGGTGTCAGGTGGCGGTACTGTGCGCCCCTGGAACTAGGGGC
CATGAAAGTCTGCTGGCAGCTTTTACTAACGCAGGATGCCTTTGTAGACGGGTGTTCTTCCGCGAAGTCA
GAGATAATACCTTTCTGGATAAGTACGTGGGACTGGCCATAGGTGGAGTGCACGGGGCTAGAGACTCAGC
TCTCGCGGGGCGAGCTACAGTGGCACTGATAAATCGCTTCCCTGCCCTCAGAGACGCTATACTGAAGTTC
CTGAATAGACCAGATACTTTTAGTGTGGCACTCGGTGAACTGGGCGTGCAGGTTCTGGCCGGCCTCGGCG
CAGTCGGGTCAACGGATAACCCACCTGCTCCAGGAGTGGAGGTTAACGTCCAGAGGTCACCCCTTATCCT
CGCTCCCAACGCTAGCGGAATGTTCGAGTCCCGGTGGCTGAACATTAGTATTCCAGCCACAACTAGCAGC
GTGATGTTGAGGGGACTGAGAGGATGCGTCCTGCCATGTTGGGTGCAGGGCTCCTGCCTCGGTCTCCAGT
TCACAAATCTGGGCATGCCGTACGTGCTGCAGAACGCCCACCAAATAGCATGTCACTTTCACTCCAACGG
CACTGACGCATGGCGGTTCGCCATGAACTACCCACGAAACCCTACAGAGCAAGGAAATATTGCTGGCCTC
TGCTCACGGGATGGTCGCCATCTTGCCCTCTTGTGTGACCCCAGCCTCTGCACGGACTTTTGGCAGTGGG
AACACATCCCGCCCGCGTTCGGACATCCAACCGGGTGTTCACCGTGGACGCTTATGTTTCAAGCTGCTCA
CCTGTGGTCCCTCCGCCATGGTCGGCCATCAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101095 representing YP_401633
Red=Cloning sites Green=Tags

MEERGRETQMPVARYGGPFIMVRLFGQDGEANIQEERLYELLSDPRSALGLDPGPLIAENLLLVALRGTN
NDPRPQRQERARELALVGILLGNGEQGEHLGTESALEASGNNYVYAYGPDWMARPSTWSAEIQQFLRLLG
ATYVLRVEMGRQFGFEVHRSRPSFRQFQAINHLVLFDNALRKYDSGQVAAGFQRALLVAGPETADTRPDL
RKLNEWVFGGRAAGGRQLADELKIVSALRDTYSGHLVLQPTETLDTWKVLSRDTRTAHSLEHGFIHAAGT
IQANCPQLFMRRQHPGLFPFVNAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVA
GFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQ
AAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGP
GMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGLNFVNDLASP
VSTAEMMDDFSPFFTVEFPPIQEEGASSPVPLDVDESMDISPSYELPWLSLESCLTSILSHPTVGSKEHL
VRHTDRVSGGRVAQQPGVGPLDLPLADYAFVAHSQVWTRPGGAPPLPYRTWDRMTEKLLVSAKPGGENVK
VSGTVITLGEQGYKVSLDLREGTRLAMAEALLNAACAPILDPEDVLLTLHLHLDPRRADNSAVMEAMTAA
SDYARGLGVKLTFGSASCPETGSSASNFMTVVASVSAPGEFSGPLITPVLQKTGSLLIAVRCGDGKIQGG
SLFEQLFSDVATTPRAPEALSLKNLFRAVQQLVKSGIVLSGHDISDGGLVTCLVEMALAGQRGVTITMPV
ASDYLPEMFAEHPGLVFEVEERSVGEVLQTLRSMNMYPAVLGRVGEQGPDQMFEVQHGPETVLRQSLRLL
LGTWSSFASEQYECLRPDRINRSMHVSDYGYNEALAVSPLTGKNLSPRRLVTEPDPRCQVAVLCAPGTRG
HESLLAAFTNAGCLCRRVFFREVRDNTFLDKYVGLAIGGVHGARDSALAGRATVALINRFPALRDAILKF
LNRPDTFSVALGELGVQVLAGLGAVGSTDNPPAPGVEVNVQRSPLILAPNASGMFESRWLNISIPATTSS
VMLRGLRGCVLPCWVQGSCLGLQFTNLGMPYVLQNAHQIACHFHSNGTDAWRFAMNYPRNPTEQGNIAGL
CSRDGRHLALLCDPSLCTDFWQWEHIPPAFGHPTGCSPWTLMFQAAHLWSLRHGRPSE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 3954 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_007605.1, YP_401633
RefSeq ORF 3954 bp
Locus ID 3783722
UniProt ID P03211
MW 142.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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