BcLF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101157

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BcLF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401697



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CNY 20,810.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101157 represents NCBI reference of YP_401697 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGCAACGAGGGTGTGGAAAACCGGCCATTTCCCTATCTGACTGTGGATGCTGACCTGTTGAGCA
ATCTGAGACAGAGTGCTGCCGAGGGACTCTTTCACAGTTTTGATCTTTTGGTGGGCAAGGATGCGCGAGA
GGCCGGTATAAAGTTTGAAGTCCTGCTGGGCGTCTACACAAATGCAATCCAGTATGTCCGATTCCTGGAG
ACAGCACTGGCCGTTAGCTGCGTGAACACCGAGTTTAAAGACCTGAGTCGGATGACTGACGGAAAGATAC
AGTTTCGCATCAGCGTGCCCACCATTGCCCATGGCGACGGTCGAAGACCCTCAAAACAGCGGACATTTAT
AGTGGTAAAGAATTGCCATAAACATCATATCTCAACCGAGATGGAGTTGAGCATGCTGGATCTCGAGATT
CTGCATAGTATCCCCGAGACCCCTGTGGAGTATGCCGAGTACGTAGGGGCAGTCAAGACAGTGGCCTCTG
CACTGCAGTTCGGTGTGGATGCGCTTGAACGAGGTCTTATTAACACGGTGTTGTCTGTGAAACTGAGGCA
TGCCCCCCCAATGTTTATCTTGCAGACCTTGGCTGACCCCACTTTTACTGAACGCGGATTTAGCAAAACA
GTGAAGAGTGACCTGATCGCAATGTTCAAACGGCATCTGTTGGAACACTCATTCTTCCTCGACAGAGCTG
AGAATATGGGAAGCGGCTTCAGTCAGTATGTGCGCTCCAGGCTCTCCGAAATGGTGGCTGCTGTGTCCGG
GGAGTCCGTCCTGAAAGGCGTTAGCACCTACACCACTGCCAAGGGAGGTGAACCGGTGGGTGGAGTTTTC
ATCGTGACAGACAACGTCCTCAGGCAGCTCCTGACTTTTCTGGGTGAGGAGGCTGACAATCAGATCATGG
GTCCGTCATCATACGCATCCTTCGTGGTGAGGGGAGAGAACCTGGTCACAGCAGTGAGCTACGGTAGGGT
GATGAGGACCTTTGAACACTTCATGGCAAGGATAGTTGATTCCCCTGAAAAAGCCGGATCAACTAAGAGC
GATCTGCCTGCCGTCGCAGCCGGAGTTGAGGACCAACCTCGCGTTCCAATTTCTGCTGCCGTGATCAAGC
TTGGCAACCACGCCGTAGCTGTGGAGTCTCTTCAGAAGATGTATAATGACACCCAATCACCATATCCACT
GAATAGGCGGATGCAGTACTCATATTACTTTCCTGTAGGCCTGTTTATGCCCAATCCTAAATACACGACA
AGTGCGGCTATCAAGATGTTGGATAATCCAACCCAACAACTCCCCGTGGAGGCCTGGATAGTAAACAAAA
ACAATCTGCTGCTGGCATTCAACCTGCAGAATGCACTGAAAGTCCTGTGTCATCCACGACTGCACACTCC
CGCTCATACACTCAATTCACTCAATGCGGCGCCTGCTCCTAGAGACCGGAGAGAGACCTATAGTCTTCAG
CACCGCAGGCCAAACCACATGAATGTACTGGTGATCGTTGATGAGTTCTATGACAACAAATATGCTGCAC
CAGTGACAGATATCGCACTCAAGTGCGGGCTCCCTACCGAAGACTTCTTGCACCCTAGTAACTATGACCT
GCTTCGGTTGGAGCTGCATCCACTCTACGACATCTACATAGGACGGGACGCTGGGGAGAGAGCCCGCCAC
AGAGCTGTCCATCGCCTGATGGTCGGGAACCTGCCAACTCCACTGGCCCCGGCAGCCTTTCAGGAGGCAA
GGGGTCAGCAGTTTGAAACCGCAACTAGTCTCGCACATGTCGTTGATCAGGCCGTCATCGAAACCGTGCA
GGACACAGCCTACGACACCGCATACCCAGCCTTTTTCTATGTGGTGGAGGCCATGATACACGGGTTCGAG
GAAAAGTTCGTGATGAATGTGCCTCTGGTCAGCCTGTGCATAAACACTTATTGGGAGCGAAGCGGACGGC
TCGCTTTCGTAAATTCATTTAGCATGATTAAATTTATTTGCCGGCACCTTGGCAACAATGCTATCAGCAA
GGAGGCTTACTCTATGTACCGCAAGATTTATGGGGAGTTGATCGCTTTGGAGCAGGCTCTCATGAGACTT
GCAGGATCCGATGTCGTGGGGGACGAGTCTGTAGGCCAATATGTATGTGCACTCCTGGATCCAAATCTGC
TGCCCCCCGTAGCCTATACTGATATCTTCACTCACCTGCTCACGGTGAGTGACAGGGCGCCGCAAATTAT
CATCGGGAATGAAGTCTATGCCGACACACTCGCTGCCCCGCAGTTCATTGAGCGCGTGGGCAACATGGAC
GAGATGGCCGCCCAATTTGTCGCCCTGTACGGATACAGAGTGAACGGGGATCACGACCACGATTTCCGCC
TGCACCTCGGTCCATACGTCGACGAGGGCCATGCAGATGTCCTGGAAAAAATTTTCTACTACGTGTTCCT
CCCAACCTGTACCAATGCCCACATGTGTGGTCTGGGGGTGGATTTTCAGCATGTGGCTCAGACACTGGCT
TACAACGGCCCCGCCTTCTCTCACCATTTCACCCGGGATGAAGATATTCTTGACAACCTTGAAAATGGAA
CTCTGCGCGATCTTCTCGAGATCAGCGATCTCAGACCTACAGTTGGCATGATAAGAGATCTCTCCGCAAG
CTTCATGACCTGCCCTACCTTCACGAGAGCCGTTAGAGTGAGCGTAGACAACGATGTCACACAGCAGCTT
GCCCCTAACCCCGCCGACAAACGGACCGAGCAAACTGTGCTGGTCAACGGGCTCGTTGCCTTTGCATTTA
GTGAACGAACCAGGGCGGTAACTCAGTGTCTGTTCCACGCTATACCTTTCCATATGTTCTATGGAGACCC
CAGGGTAGCAGCCACGATGCACCAAGATGTCGCCACTTTCGTGATGAGGAACCCTCAACAGAGGGCAGTG
GAAGCCTTCAACCGCCCAGAGCAACTTTTCGCCGAGTATCGGGAATGGCACCGCAGTCCGATGGGGAAGT
ACGCGGCTGAGTGTCTGCCTTCCCTGGTGTCCATCAGCGGCATGACAGCCATGCACATAAAAATGTCCCC
GATGGCCTATATCGCGCAGGCAAAATTGAAGATTCACCCAGGGGTTGCCATGACTGTCGTAAGAACAGAC
GAAATTTTGTCCGAGAATATTCTGTTCAGCTCCAGAGCCTCTACCTCTATGTTTATTGGAACACCCAATG
TCTCCCGGAGGGAAGCGCGGGTCGATGCTGTTACCTTCGAGGTGCACCATGAGATGGCCAGCATCGATAC
AGGACTGAGCTATTCTAGTACAATGACACCTGCTAGGGTGGCTGCCATAACAACTGATATGGGAATTCAT
ACCCAGGACTTTTTCAGCGTGTTCCCAGCAGAAGCATTCGGCAACCAGCAGGTGAATGACTATATCAAAG
CCAAGGTTGGTGCCCAAAGGAACGGGACGCTCCTCAGAGATCCCCGGACCTACCTCGCCGGCATGACTAA
CGTTAATGGAGCTCCGGGCCTTTGTCATGGGCAGCAAGCCACCTGCGAGATTATTGTGACCCCGGTGACT
GCAGACGTGGCCTACTTTCAGAAATCCAATAGTCCTCGCGGCCGCGCGGCTTGCGTGGTTAGTTGCGAGA
ATTACAACCAAGAGGTCGCTGAGGGTCTGATTTATGACCATAGCCGCCCAGACGCCGCGTATGAGTATAG
AAGCACAGTGAATCCTTGGGCTTCACAGCTGGGTAGTCTTGGAGACATTATGTACAACTCTAGCTACCGC
CAGACTGCTGTCCCAGGACTGTACAGCCCTTGTCGCGCCTTCTTCAACAAGGAAGAGCTGCTTAGAAATA
ACAGAGGACTGTATAATATGGTCAATGAGTACAGTCAGAGATTGGGGGGTCACCCTGCCACTAGCAATAC
AGAAGTGCAGTTCGTGGTGATCGCCGGAACGGATGTTTTTTTGGAACAACCATGCAGTTTCCTGCAGGAG
GCCTTTCCCGCTCTGTCCGCCTCTTCCAGAGCGTTGATCGACGAATTCATGTCCGTTAAGCAGACCCACG
CACCCATCCATTATGGCCATTATATTATCGAGGAGGTAGCTCCAGTTCGGCGAATACTCAAATTTGGCAA
TAAAGTGGTGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101157 representing YP_401697
Red=Cloning sites Green=Tags

MASNEGVENRPFPYLTVDADLLSNLRQSAAEGLFHSFDLLVGKDAREAGIKFEVLLGVYTNAIQYVRFLE
TALAVSCVNTEFKDLSRMTDGKIQFRISVPTIAHGDGRRPSKQRTFIVVKNCHKHHISTEMELSMLDLEI
LHSIPETPVEYAEYVGAVKTVASALQFGVDALERGLINTVLSVKLRHAPPMFILQTLADPTFTERGFSKT
VKSDLIAMFKRHLLEHSFFLDRAENMGSGFSQYVRSRLSEMVAAVSGESVLKGVSTYTTAKGGEPVGGVF
IVTDNVLRQLLTFLGEEADNQIMGPSSYASFVVRGENLVTAVSYGRVMRTFEHFMARIVDSPEKAGSTKS
DLPAVAAGVEDQPRVPISAAVIKLGNHAVAVESLQKMYNDTQSPYPLNRRMQYSYYFPVGLFMPNPKYTT
SAAIKMLDNPTQQLPVEAWIVNKNNLLLAFNLQNALKVLCHPRLHTPAHTLNSLNAAPAPRDRRETYSLQ
HRRPNHMNVLVIVDEFYDNKYAAPVTDIALKCGLPTEDFLHPSNYDLLRLELHPLYDIYIGRDAGERARH
RAVHRLMVGNLPTPLAPAAFQEARGQQFETATSLAHVVDQAVIETVQDTAYDTAYPAFFYVVEAMIHGFE
EKFVMNVPLVSLCINTYWERSGRLAFVNSFSMIKFICRHLGNNAISKEAYSMYRKIYGELIALEQALMRL
AGSDVVGDESVGQYVCALLDPNLLPPVAYTDIFTHLLTVSDRAPQIIIGNEVYADTLAAPQFIERVGNMD
EMAAQFVALYGYRVNGDHDHDFRLHLGPYVDEGHADVLEKIFYYVFLPTCTNAHMCGLGVDFQHVAQTLA
YNGPAFSHHFTRDEDILDNLENGTLRDLLEISDLRPTVGMIRDLSASFMTCPTFTRAVRVSVDNDVTQQL
APNPADKRTEQTVLVNGLVAFAFSERTRAVTQCLFHAIPFHMFYGDPRVAATMHQDVATFVMRNPQQRAV
EAFNRPEQLFAEYREWHRSPMGKYAAECLPSLVSISGMTAMHIKMSPMAYIAQAKLKIHPGVAMTVVRTD
EILSENILFSSRASTSMFIGTPNVSRREARVDAVTFEVHHEMASIDTGLSYSSTMTPARVAAITTDMGIH
TQDFFSVFPAEAFGNQQVNDYIKAKVGAQRNGTLLRDPRTYLAGMTNVNGAPGLCHGQQATCEIIVTPVT
ADVAYFQKSNSPRGRAACVVSCENYNQEVAEGLIYDHSRPDAAYEYRSTVNPWASQLGSLGDIMYNSSYR
QTAVPGLYSPCRAFFNKEELLRNNRGLYNMVNEYSQRLGGHPATSNTEVQFVVIAGTDVFLEQPCSFLQE
AFPALSASSRALIDEFMSVKQTHAPIHYGHYIIEEVAPVRRILKFGNKVVF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 4143 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_007605.1, YP_401697
RefSeq ORF 4143 bp
Locus ID 3783688
UniProt ID P03211
MW 153.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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