BBLF2/BBLF3 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101144

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BBLF2/BBLF3 [Human herpesvirus 4], codon optimized for human cell expression, YP_401684



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CNY 10,640.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101144 represents NCBI reference of YP_401684 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAACGCCAGCTGAGTCAGTGCGCGCCCGCGTATCATCTGTCACTTTTTATAATGTTACTCAAA
CAGCCGGCAGGTGGTGGGCCATCTGGGTGGTGGGGATCGTTCCGATTAAGCGGGAGGATGTTGAGACCCT
CATCGTGGTCCAGGCCTGCCAACCTCCTCTGGGTGGCTCCCTTGAGCCCCCCGTGGTTAATGCACCTAGC
ACAACTGAACTGAACTTTTTGAGATGGGAACGAGAACTGAGACGGTCAGGCGGCCTCATAGCAATGCTGG
CTGACGCTGCAGAGAAAGATCTTTTTGACTTGAGTTTTCGGACACGGGACCGGCGGCTGCTCTCCGCTGC
TAGAGTTGAGGATGAACAGGGCTTGATTTTCCAGCCTCTCTTTCCGGCCCAAGTAGTGTGCCAATCTTGC
AGTGGCGATGACGGGCGGGATCAACAGCCTCCCCCTGTAGACGGGTTTGGATCAGAGATGGAGGGGGAGC
AAACGTGCCCGCACGCCCAGAGACACAGTGAGAGTCCTGGCCAGCTTGACGTGTATATTCGCACCCCCCG
GGGGGACGTGTTTACCTACTCAACCGAAACACCAGATGACCCTTCACCTGTGCCCTTCCGGGACATACTG
CGCCCAGTAACCTATGAAGTTGACCTGGTCTCTTCAGATGGTGCCACCGGGCGGGGCGGGGATGCCAGAC
GGCATAGAGTCTCTTTGAAGATTCTGGAGCCAGCTGGCGGTTTCGAATCCTGGTTGGTCAACAGCTGGAG
CATGGCTGGTGGGGGTCTCTACGCTTTCCTTAGAAGTATTTATGCTTCTTGTTACGCCAACCATAGAGGT
ACCAAGCCAATATTCTACCTCTTGGACCCGGAGCTGTGTCCTGGGGGATCTGACTTTCAGCCCTATGTGC
CAGGCTTCCCTTTCCTCCCAATCCACTATGTCGGGCGCGCGAGGCCGGCCTTCTGGCACCGCGCCCCACA
CTCAGAGGGACTGCTGCTTTTGGATCTGAATCTTGGAGTGAGCGGTACACCCCTTGCGGACGCGCTTCTT
GGGCTCGATGCACGCTCCGGGCAACGCAGAGGATCTTTGCTTTTGCAGCAGATCTGGCCACCTACTCGCA
AGGAGATAAATCCCCGGCATGTTTGCACACGCGAGGGGGGGGAGGGCGGTGGAGAAGATGAAACTACCGT
GGTCGGGCGGGCTGAAGCCACCGCCATCCTCGAAGCCGACGCCACATGGTGGCTGTATGAATTGGCTCGC
TGTCACCTCAGCGCTCGGGGAGCCCCTGTAGGCACCCCTGATGGGGGGGGACAGGCACGAGATGCTCAGA
CTTGGCTGAGGGCTCTCCATAGGTATGGTACTTCCGACACACGAAGGGCTCTGGGTGGCCTCTACACAGC
TGTGACCCGGGTGTTGCTGCATGCGGCAGCCGATCTGGGCCTGACCTGGGCCTACGCGGACGAGTTTATC
CTTGGCTTCGTGGCCCCTACAAGCGCACATCCTAGTGAAGAACCTCTCGCGCAGGCTTTCCTCCAAGGAG
TTAAGGACAGCGAGGATGCATCCCGGCTGGATCGAGATGTAATGGGAGGAGAGGCTACCGTCGCTCGCCG
ACACATCAGGGTCAAGGCGCGACGCGGGCCAGGTTGTCTCCTGATGGCTATCTTCCAGGGCGATTTGTAT
GTTGGGGGATGTCGCGAGCATTCTGGCCCCTTCCTCGTGTGGCACGAGGCATTCAGCTGGACACTCGACC
AACTGGCGGCTAGACCCGAGGCCGACAAAGCGCCACCATCCCACGATCATCTGCTCACCTTGGTAAGAGA
TCTCACCAGGCGCCTCGCTCCCGGAAGACGCCGCAACAGATTTTGGGCACTCCCCCGAGCCTGGCTGCAG
AGGCTGAGACGGGCTGGTCTGCGACTCTCTGGGAGCCACGTTTGCCTTCTTGACAAGGATGGCGCTCGCC
CTGCCCCCTGTCAAACCGCGACCGAGCACGGGCTGAGTCCCACGGCATACTTCCGCGAAATCATGGCCTT
TCTTCTGGACGTTATTAGCGCACTCCATCCGGGGTATACGATCCCCATGGAAATAACCCGCGAAACTGAC
CTGCTGATGACAGTGTTGTCCCTCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101144 representing YP_401684
Red=Cloning sites Green=Tags

MMETPAESVRARVSSVTFYNVTQTAGRWWAIWVVGIVPIKREDVETLIVVQACQPPLGGSLEPPVVNAPS
TTELNFLRWERELRRSGGLIAMLADAAEKDLFDLSFRTRDRRLLSAARVEDEQGLIFQPLFPAQVVCQSC
SGDDGRDQQPPPVDGFGSEMEGEQTCPHAQRHSESPGQLDVYIRTPRGDVFTYSTETPDDPSPVPFRDIL
RPVTYEVDLVSSDGATGRGGDARRHRVSLKILEPAGGFESWLVNSWSMAGGGLYAFLRSIYASCYANHRG
TKPIFYLLDPELCPGGSDFQPYVPGFPFLPIHYVGRARPAFWHRAPHSEGLLLLDLNLGVSGTPLADALL
GLDARSGQRRGSLLLQQIWPPTRKEINPRHVCTREGGEGGGEDETTVVGRAEATAILEADATWWLYELAR
CHLSARGAPVGTPDGGGQARDAQTWLRALHRYGTSDTRRALGGLYTAVTRVLLHAAADLGLTWAYADEFI
LGFVAPTSAHPSEEPLAQAFLQGVKDSEDASRLDRDVMGGEATVARRHIRVKARRGPGCLLMAIFQGDLY
VGGCREHSGPFLVWHEAFSWTLDQLAARPEADKAPPSHDHLLTLVRDLTRRLAPGRRRNRFWALPRAWLQ
RLRRAGLRLSGSHVCLLDKDGARPAPCQTATEHGLSPTAYFREIMAFLLDVISALHPGYTIPMEITRETD
LLMTVLSLF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 2127 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_007605.1, YP_401684
RefSeq ORF 2127 bp
Locus ID 3783684
UniProt ID P03211
MW 78.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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