BALF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101177

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BALF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401717



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CNY 17,010.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101177 represents NCBI reference of YP_401717 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGGCGCACAGACTAGTGAAGACAACCTTGGCTCCCAGTCACAGCCCGGACCATGCGGGTACATCT
ATTTTTACCCCCTGGCGACCTACCCTCTCAGGGAAGTCGCTACTTTGGGCACAGGCTATGCCGGCCACCG
GTGCCTGACTGTGCCCCTGCTTTGCGGAATTACCGTAGAGCCAGGTTTCAGTATCAACGTAAAGGCCTTG
CACAGACGCCCTGACCCGAATTGTGGCCTGCTGCGCGCCACCTCCTATCACCGAGATATATACGTCTTTC
ACAACGCGCACATGGTTCCACCAATATTCGAAGGACCTGGACTCGAAGCCCTGTGTGGGGAGACGAGAGA
GGTGTTTGGGTACGACGCATACAGTGCTCTCCCCCGAGAGAGTAGCAAACCCGGTGACTTCTTTCCGGAG
GGTCTGGATCCCAGTGCGTACCTGGGGGCCGTCGCCATCACCGAGGCTTTCAAAGAGCGGCTGTATAGCG
GTAATCTGGTCGCCATTCCCAGCCTCAAGCAGGAGGTCGCCGTTGGTCAGTCAGCCTCTGTGCGCGTCCC
ACTTTACGATAAGGAGGTCTTCCCCGAAGGGGTCCCTCAGCTTAGACAGTTTTATAACTCAGACTTGAGC
CGGTGCATGCACGAGGCCCTGTATACCGGCCTGGCACAGGCCCTCAGAGTACGCAGAGTTGGTAAATTGG
TGGAGCTGCTGGAAAAGCAGTCTCTGCAAGACCAGGCCAAGGTCGCCAAGGTGGCTCCCCTGAAGGAATT
TCCCGCATCAACAATCTCACATCCCGATAGCGGCGCCCTTATGATTGTAGATTCAGCAGCGTGCGAACTG
GCTGTTTCTTACGCCCCCGCCATGCTCGAGGCGAGCCACGAGACTCCCGCAAGCTTGAACTACGACTCCT
GGCCCTTGTTTGCCGACTGCGAAGGACCCGAAGCCAGAGTCGCTGCCCTGCACCGGTATAATGCCAGTCT
GGCCCCACACGTTTCTACGCAAATATTCGCCACCAACAGTGTCTTGTATGTGAGCGGAGTGAGCAAATCT
ACTGGCCAGGGCAAGGAGAGCTTGTTTAATAGCTTCTATATGACCCATGGACTCGGAACCCTCCAAGAGG
GTACTTGGGACCCCTGCCGGCGCCCATGCTTCAGCGGATGGGGTGGGCCAGATGTCACTGGAACGAACGG
CCCCGGCAACTACGCAGTGGAACACTTGGTGTACGCGGCCTCTTTTAGTCCGAACCTTCTCGCTCGATAT
GCTTATTACCTGCAGTTTTGTCAGGGCCAGAAGAGCAGCCTCACCCCCGTTCCTGAAACCGGATCTTACG
TTGCAGGGGCCGCAGCCAGCCCGATGTGTTCCCTTTGCGAAGGCCGCGCTCCAGCTGTCTGTCTGAATAC
ACTGTTCTTTCGACTGCGCGATCGATTCCCGCCCGTGATGTCTACCCAGAGACGGGACCCTTATGTGATT
AGCGGCGCCTCCGGCTCATATAATGAAACAGATTTCCTCGGCAACTTCTTGAACTTTATCGATAAGGAGG
ACGACGGCCAGAGGCCGGATGACGAGCCCCGCTATACTTACTGGCAGCTGAACCAAAACCTCCTTGAGCG
CCTCAGTAGGCTTGGTATCGACGCTGAAGGCAAGCTCGAGAAGGAGCCACACGGTCCGAGAGACTTCGTG
AAGATGTTCAAGGACGTCGACGCCGCCGTCGATGCAGAGGTCGTGCAGTTCATGAACTCCATGGCTAAAA
ACAACATAACTTATAAAGACCTGGTTAAGTCTTGCTATCATGTCATGCAGTATAGCTGCAATCCATTTGC
CCAGCCCGCTTGCCCTATTTTCACACAGCTTTTTTATAGGTCCCTCCTCACAATTCTGCAGGATATCTCT
CTGCCGATATGCATGTGTTACGAGAACGATAACCCGGGCCTGGGACAAAGTCCGCCTGAGTGGCTGAAGG
GCCACTACCAAACTCTGTGCACCAATTTCCGCAGCTTGGCCATCGATAAAGGCGTTCTCACGGCCAAAGA
GGCTAAAGTCGTCCATGGCGAACCCACTTGTGACCTTCCCGACCTGGACGCGGCTTTGCAGGGTAGGGTG
TACGGTCGGCGGCTGCCCGTTAGGATGTCTAAGGTGTTGATGTTGTGTCCTAGAAACATTAAAATCAAGA
ATAGAGTTGTGTTCACAGGAGAGAATGCCGCACTGCAAAACAGTTTCATTAAATCAACTACACGCCGGGA
AAATTACATAATCAATGGCCCTTACATGAAATTTCTCAACACATATCACAAGACCCTTTTTCCGGATACC
AAGCTGAGCTCTCTCTATCTCTGGCATAATTTCAGTCGCCGGCGGTCTGTGCCAGTACCATCAGGAGCAT
CCGCTGAAGAGTACAGCGATCTCGCACTGTTTGTTGATGGCGGTAGCAGAGCCCACGAAGAGTCAAATGT
TATTGACGTGGTGCCTGGTAACCTGGTTACGTACGCGAAACAGCGGCTTAATAACGCCATATTGAAAGCA
TGTGGCCAAACTCAGTTTTACATTTCCCTCATCCAGGGACTTGTGCCTCGGACTCAATCTGTACCTGCTC
GCGACTACCCTCATGTGTTGGGGACCAGGGCGGTGGAAAGTGCCGCGGCTTACGCCGAAGCTACTAGCTC
CCTGACCGCTACTACTGTCGTTTGCGCCGCCACAGACTGTCTCTCACAAGTATGCAAAGCTCGGCCCGTG
GTTACACTCCCTGTAACAATTAATAAATACACTGGTGTTAACGGGAACAACCAGATATTCCAGGCAGGAA
ATCTTGGCTACTTCATGGGCAGGGGCGTGGATAGGAACCTGCTGCAAGCTCCAGGTGCAGGTCTGCGAAA
GCAGGCAGGAGGGAGCTCTATGAGGAAGAAGTTCGTCTTTGCTACACCCACACTGGGGCTGACAGTGAAA
AGGCGCACACAAGCTGCCACCACCTACGAGATAGAGAATATTCGAGCCGGACTGGAAGCTATTATCTCAC
AAAAACAGGAGGAGGATTGCGTGTTTGATGTAGTATGCAATCTTGTTGACGCGATGGGGGAGGCTTGTGC
ATCCCTCACCCGGGACGATGCCGAATACCTGCTGGGAAGGTTTTCCGTGCTTGCCGATTCAGTCCTTGAG
ACTTTGGCAACCATTGCCTCAAGTGGGATCGAATGGACCGCCGAAGCCGCGCGCGACTTCTTGGAGGGAG
TGTGGGGCGGACCCGGTGCCGCCCAGGACAACTTCATCAGTGTCGCTGAGCCTGTGTCTACCGCAAGTCA
AGCTTCTGCTGGACTGCTCCTTGGCGGCGGTGGCCAGGGATCCGGGGGCAGGAGGAAGAGACGCCTGGCC
ACAGTGCTGCCCGGCCTTGAAGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101177 representing YP_401717
Red=Cloning sites Green=Tags

MQGAQTSEDNLGSQSQPGPCGYIYFYPLATYPLREVATLGTGYAGHRCLTVPLLCGITVEPGFSINVKAL
HRRPDPNCGLLRATSYHRDIYVFHNAHMVPPIFEGPGLEALCGETREVFGYDAYSALPRESSKPGDFFPE
GLDPSAYLGAVAITEAFKERLYSGNLVAIPSLKQEVAVGQSASVRVPLYDKEVFPEGVPQLRQFYNSDLS
RCMHEALYTGLAQALRVRRVGKLVELLEKQSLQDQAKVAKVAPLKEFPASTISHPDSGALMIVDSAACEL
AVSYAPAMLEASHETPASLNYDSWPLFADCEGPEARVAALHRYNASLAPHVSTQIFATNSVLYVSGVSKS
TGQGKESLFNSFYMTHGLGTLQEGTWDPCRRPCFSGWGGPDVTGTNGPGNYAVEHLVYAASFSPNLLARY
AYYLQFCQGQKSSLTPVPETGSYVAGAAASPMCSLCEGRAPAVCLNTLFFRLRDRFPPVMSTQRRDPYVI
SGASGSYNETDFLGNFLNFIDKEDDGQRPDDEPRYTYWQLNQNLLERLSRLGIDAEGKLEKEPHGPRDFV
KMFKDVDAAVDAEVVQFMNSMAKNNITYKDLVKSCYHVMQYSCNPFAQPACPIFTQLFYRSLLTILQDIS
LPICMCYENDNPGLGQSPPEWLKGHYQTLCTNFRSLAIDKGVLTAKEAKVVHGEPTCDLPDLDAALQGRV
YGRRLPVRMSKVLMLCPRNIKIKNRVVFTGENAALQNSFIKSTTRRENYIINGPYMKFLNTYHKTLFPDT
KLSSLYLWHNFSRRRSVPVPSGASAEEYSDLALFVDGGSRAHEESNVIDVVPGNLVTYAKQRLNNAILKA
CGQTQFYISLIQGLVPRTQSVPARDYPHVLGTRAVESAAAYAEATSSLTATTVVCAATDCLSQVCKARPV
VTLPVTINKYTGVNGNNQIFQAGNLGYFMGRGVDRNLLQAPGAGLRKQAGGSSMRKKFVFATPTLGLTVK
RRTQAATTYEIENIRAGLEAIISQKQEEDCVFDVVCNLVDAMGEACASLTRDDAEYLLGRFSVLADSVLE
TLATIASSGIEWTAEAARDFLEGVWGGPGAAQDNFISVAEPVSTASQASAGLLLGGGGQGSGGRRKRRLA
TVLPGLEV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 3384 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_007605.1, YP_401717
RefSeq ORF 3384 bp
Locus ID 3783678
UniProt ID P03211
MW 123.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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